We narrowed to 34,664 results for: cas

1. pSCAR_Cas9-hygro_GFP

   Plasmid

   #162075

   Purpose

   3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Inserts

   Cas9-2a-hygromycin B phosphotransferase
   EGFP
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in M. musculus

   Promoter

   EF1a and sv40

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. BB3cK_pGAP_23*_pTEF_Cas9

   Plasmid

   #104909

   Purpose

   hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-hCas9D10A

   Plasmid

   #52101

   Purpose

   The expression vector for humanized nickase Cas9 (Cas9D10A) under CAG promoter

   Depositor

   Izuho Hatada

   Insert

   hCas9D10A
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   CAG

   Available since

   April 30, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pAAV.pU1a-SpCas9

   Plasmid

   #121507

   Purpose

   Expresses codon-optimized SpCas9 in mammalian cells.

   Depositor

   Guangping Gao

   Article

   Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

   Insert

   SpCas9
   

   Use

   AAV

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   U1a

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLX311-Cas9

   Plasmid

   #118018

   Purpose

   Constitutive expression of Cas9

   Depositor

   David Root, William Hahn

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   Cas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21b-Caspase-3

   Plasmid

   #90087

   Purpose

   Expresses wild type caspase 3 in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Wild type

   Promoter

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. TadCBE-Cas9NG

   Plasmid

   #225093

   Purpose

   Plasmid for bacterial purification of codon-optimized TadCBE-Cas9NG

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Science. 2025 Apr 4;388(6742):52-59. doi

   Insert

   TadCBE-Cas9NG
   

   Use

   CRISPR

   Tags

   8xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEVA421-Cas9tr

   Plasmid

   #138709

   Purpose

   Plasmid constitutively expressing the Cas9 nuclease and the non-coding tracrRNA from S. pyogenes

   Depositor

   Víctor de Lorenzo

   Article

   Velazquez et al ACS Synth Biol. 2019 Sep 20;8(9):2186-21

   Insert

   tracrRNA-Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. KRAB-dSpCas9

   Plasmid

   #205248

   Purpose

   In vitro transcription plasmid for production of KOX1 KRAB-dSpCas9 mRNA for transcriptional repression (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #85449.

   Depositor

   Rasmus Bak

   Article

   Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

   Insert

   KRAB-dSpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpdCas9 KRAB

    Plasmid

    #188499

    Purpose

    Expresses FLAG tagged SpdCas9 KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A/H840A

    Promoter

    EF1a

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti.Cas9.BFP.Blast

    Plasmid

    #196714

    Purpose

    WT SpCas9 with BSD-TagBFP for selection and sorting

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. RCAS GFP (CT#22)

    Plasmid

    #13878

    Depositor

    Connie Cepko

    Article

    Chen et al Dev Biol. 1999 Oct 15. 214(2):370-84.

    Insert

    GFP
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHdzCas9-KRAB

    Plasmid

    #92341

    Purpose

    Expression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistance

    Depositor

    I-Son Ng

    Article

    Kao et al Bioresour Technol. 2017 May 4. pii: S096

    Type

    Empty backbone

    Use

    CRISPR; Plant expression

    Tags

    KRAB

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas9-CtIP

    Plasmid

    #109403

    Purpose

    Expresses Cas9 fusion to human CtIP protein

    Depositor

    Jean-Paul Concordet

    Article

    Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

    Insert

    CtIP (RBBP8 Human)
    

    Use

    Tags

    Expression

    Mutation

    full length CtIP

    Promoter

    Available since

    June 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQdCas9-sgempty

    Plasmid

    #159523

    Purpose

    Expression of dCas9 driven by lux cassette from Vibrio fischeri

    Depositor

    Radhakrishnan Mahadevan

    Article

    Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

    Insert

    quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    quorum sensing promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL0284 (pQCascade_entry)

    Plasmid

    #130635

    Purpose

    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array contains two BsaI sites for spacer cloning.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(entry)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRISPR-dCas9

    Plasmid

    #125687

    Purpose

    Gene knockdown for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

    Insert

    streptomyces codon optimized spdCas9, sgRNA casette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    ermE*/tipA

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO5d.SFFV.dCas9-KRAB.P2A.BSD

    Plasmid

    #90332

    Purpose

    CRISPR interference

    Depositor

    Dirk Heckl

    Article

    Schwarzer et al Nat Commun. 2017 Aug 9;8(1):218. doi: 10

    Inserts

    dCas9
    na
    SFFV
    P2A-BSD
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CL7-LbCas12a

    Plasmid

    #164658

    Purpose

    Bacterial expression of LbCas12a using CL7/Im7

    Depositor

    Piyush Jain

    Article

    Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

    Insert

    LbCas12a
    

    Use

    Tags

    Thioredoxin, CL7, SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. CMV-enAsCas12f

    Plasmid

    #204637

    Purpose

    Expression of enAsCas12f in mammalian cells

    Depositor

    Weixin Tang

    Article

    Wu et al Nat Chem Biol. 2023 Jul 3. doi: 10.1038/

    Insert

    enAsCas12f
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    D196K, N199K, G276R, N328G, D364R

    Promoter

    CMV

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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