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dCas9 plasmid

Plasmid

#100091

Purpose

dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Publication

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Type

Empty backbone

Use

CRISPR

Tags

3XFlag-NLS-dCas9-NLS

Expression

Mammalian

Promoter

CMV

Available since

Oct. 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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p3962 pcDNA3-IRF3-FL

Plasmid

#22860

Depositor

Peter Howley

Publication

Kirshner et al J Virol. 2005 Jul . 79(14):9320-4.

Insert

IRF3 (IRF3 Human)

Expression

Mammalian

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDEST/LIfeAct-mCherry-N1

Plasmid

#40908

Depositor

Robin Shaw

Publication

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

LifeAct

Tags

mCherry

Expression

Mammalian

Mutation

No stop codon to create c-terminal fusion

Promoter

CMV

Available since

Nov. 21, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pCMV-myc-LC3 (Atg8)

Plasmid

#24919

Depositor

Toren Finkel

Publication

Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

Insert

LC3 (MAP1LC3B Human)

Tags

Myc

Expression

Mammalian

Available since

June 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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Dominant negative Hsp90 beta

Plasmid

#22480

Depositor

William Sessa

Publication

Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

Insert

Dominant negative Hsp90 (HSP90AB1 Human)

Tags

HA

Expression

Mammalian

Available since

Dec. 8, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pWZL hygro H-Ras V12

Plasmid

#18749

Depositor

Scott Lowe

Publication

Serrano et al Cell. 1997 Mar 7. 88(5):593-602.

Insert

H-Ras V12 (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

V12

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

20 Articles

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pcDNA4TO-24xGCN4_v4-kif18b-24xPP7

Plasmid

#74928

Purpose

Translation reporter

Depositor

Marvin Tanenbaum

Publication

Yan et al Cell. 2016 May 5;165(4):976-89. doi: 10.

Insert

24xGCN4_v4-kif18b-24xPP7

Expression

Mammalian

Available since

May 10, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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PCMV-flag-Caspase11

Plasmid

#21145

Depositor

Junying Yuan

Publication

Li et al Nat Cell Biol. 2007 Mar . 9(3):276-86.

Insert

Caspase11 (Casp4 Mouse)

Tags

flag

Expression

Mammalian

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pCMV6M-PAK1 T423E

Plasmid

#12208

Depositor

Jonathan Chernoff

Publication

Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

Insert

Pak1 (PAK1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Activated Pak1: T423E

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

18 Articles

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mouse E-cadherin GFP (423)

Plasmid

#67937

Purpose

Mammalian expression of E-cadherin-GFP

Depositor

Alpha Yap

Publication

Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

Insert

E-cadherin (Cdh1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pLSSmOrange-mKate2 caspase-3 biosensor

Plasmid

#37132

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

Insert

LSSmOrange-DEVD-mKate2

Expression

Mammalian

Mutation

A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

Promoter

CMV

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EGFPC1-hMyoX

Plasmid

#47608

Depositor

Emanuel Strehler

Publication

Bennett et al J Biol Chem. 2007 Feb 2;282(5):3205-12.

Insert

MyoX (MYO10 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Mutation

See depositor comments below.

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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APEX2 - Actin in pEGFP

Plasmid

#66172

Purpose

APEX2 fused to the N-terminus of human beta actin

Depositor

Alice Ting

Publication

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2 - human beta Actin (ACTB Synthetic, Human)

Tags

Flag epitope tag (DYKDDDDK)

Expression

Mammalian

Mutation

APEX2 contains 4 mutations relative to wt soybean…

Promoter

CMV

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pBabe EGFR(T790M)

Plasmid

#32070

Depositor

Matthew Meyerson

Publication

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

T790M

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pLenti6/V5/DEST-VPS35

Plasmid

#21691

Depositor

Lynda Chin

Publication

Scott et al Nature. 2009 Jun 25. 459(7250):1085-90.

Insert

Vacuolar Protein Sorting 35 homolog (VPS35 Human)

Use

Lentiviral; Invitrogen gateway destination vector

Tags

V5

Expression

Mammalian

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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G6PD/pRK5

Plasmid

#41521

Depositor

Xiaolu Yang

Publication

Jiang et al Nat Cell Biol. 2011 Mar;13(3):310-6. Epu

Insert

glucose-6-phosphate dehydrogenase (G6PD Human)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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FRB-NLuc

Plasmid

#31181

Depositor

David Piwnica-Worms

Publication

Luker et al Proc Natl Acad Sci U S A. 2004 Aug 17. 1

Insert

Rapamycin-binding domain and the N-terminus of Firefly Luciferase (MTOR Human)

Expression

Mammalian

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PKC alpha CAT

Plasmid

#21234

Depositor

Bernard Weinstein

Publication

Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

Insert

PKC alpha (PRKCA Human)

Tags

HA

Expression

Mammalian

Mutation

delta 1-325

Available since

Aug. 13, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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p3XFlag-CAPN2

Plasmid

#60942

Purpose

Expresses calpain2 in mammalian cells

Depositor

Yi Zhang

Publication

Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

Insert

Calpain 2 (CAPN2 Human)

Tags

3xflag

Expression

Mammalian

Promoter

CMV

Available since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pAW12.lentiguide.GFP

Plasmid

#104374

Purpose

GFP vector for cloning in guides

Depositor

Richard Young

Publication

Weintraub et al Cell. 2017 Dec 14;171(7):1573-1588.e28.

Type

Empty backbone

Use

Lentiviral

Available since

Jan. 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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