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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 7981 - 8000 of 14031 results

  3. pCas9

    Plasmid
    #167547
    Purpose
    Used for CRISPR-Cas9 mediated recombineering in Enterococcus. Can clone desired gRNA using BsaI digestion.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Inserts
    chloramphenicol acetyl transferase
    pUC19 origin of replication
    Use
    CRISPR
    Expression
    Bacterial
    Available Since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167547-plasmid-map-sequence-id-332733

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  4. hCas9_D10A

    Plasmid
    #41816
    Purpose
    Expresses human codon optimized Cas9 D10A mutant which functions as a nickase for genome engineering
    Depositor
    George Church
    Article
    Mali et al Science. 2013 Feb 15;339(6121):823-6. do
    Insert
    Cas9_D10A
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A nickase
    Promoter
    CMV
    Available Since
    Jan. 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    41816-plasmid-map-sequence-id-116715

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  17. DT044A

    Plasmid
    #159405
    Purpose
    Inducible Perfringolysin O (PFO) expression and purification, controlled under hybrid IPTG-regulated T7/LacO promoter (pRT30)
    Depositor
    Sheref Mansy
    Article
    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea
    Insert
    pr.T7-LacO-6xHis-PFO (Pfo, Clostridium perfringens)
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    T7/LacO promoter
    Available Since
    Feb. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    159405-plasmid-map-sequence-id-324289

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Showing: 7981 - 8000 of 14031 results