We narrowed to 2,130 results for: piggyBac

Showing: 801 - 820 of 2130 results

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pPB-CAG-3xFLAG::Fnip1-pgk-hph

Plasmid

#48758

Purpose

PiggyBac backbone, CAG promoter driving murine 3xFLAG-Fnip1 fusion, Hygromycin selection

Depositor

Austin Smith

Article

Betschinger et al Cell. 2013 Apr 11;153(2):335-47. doi: 10

Insert

3xFLAG::Fnip1 (Fnip1 Mouse)

Use

Piggybac

Tags

3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only
pPB-cT3G-cERH-BCL2

Plasmid

#222583

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of BCL2, with hygromycin resistance

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

BCL2 (BCL2 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJPF0190

Plasmid

#130268

Purpose

Piggybac vector for the expression of mRuby2-fused human PRKACA

Depositor

Hana El-Samad

Article

Fonseca et al bioRxiv 2020, 747030

Insert

PRKACA (PRKACA Human)

Tags

mRuby2

Expression

Mammalian

Promoter

CAG

Available Since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP

Plasmid

#233445

Purpose

PiggyBac donor plasmid to overexpress a full length ADAR2

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

ADAR2-T2A-PuroR-P2A-BFP

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pTS26_QUAS_CsChrimson

Plasmid

#175548

Purpose

Expresses the optogenetic channel CsChrimson off of a QUAS promoter and contains Piggybac terminal repeats for transposon-mediated integration

Depositor

Leslie Vosshall

Article

Sorrells et al Elife. 2022 May 12;11:e76663. doi: 10.75

Insert

CsChrimson

Tags

tdTomato

Expression

Insect

Promoter

QUAS

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pPB-R1R2-EM7neoPheS

Plasmid

#112923

Purpose

piggyBac Transposon vector with a Gateway destination cassette (neoPheS selection)

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

neoPheS

Use

Piggybac transposon gateway destination vector

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PB-Neo-TetO-Cdk12_Untagged

Plasmid

#127178

Purpose

Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

Depositor

Phil Sharp

Article

Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

Insert

Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)

Use

Piggybac transposase vector

Tags

None

Expression

Mammalian

Promoter

TetO Promoter

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT_GFP 150TAG

Plasmid

#140016

Purpose

Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Expression

Mammalian

Mutation

150TAG, K46E (Please see depositor comments below)

Promoter

EF1

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pB-Gaolf-Integration

Plasmid

#129456

Purpose

piggyBac transposon vector that Inducibly (Tet-On) expresses Gaolf

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

G alpha olf (Gnal Mouse)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE (Tet-On)

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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KX002: pMVP/PB-DEST

Plasmid

#121869

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pAS_FLAG-Mma PylRS

Plasmid

#154762

Purpose

plasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mma PylRS (pylS Methanosarcina mazei)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pEPIC1.1

Plasmid

#169897

Purpose

Empty constitutive expression vector with piggyBac, IRES, and mClover3

Depositor

Richard Schneider

Article

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Avian expression

Tags

3x FLAG 2x StrepII

Expression

Mammalian

Promoter

mouse PGK

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pUC57-PB-PGK-tetR-3xFLAG-HA-2A-Puro

Plasmid

#129537

Purpose

PiggyBac transposon carrying tetR-3xFLAG-HA-2A-Puro under the PGK promoter.

Depositor

Hideyuki Okano

Article

Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

Insert

tetR-3xFlag-HA-2A-Puro

Use

Synthetic Biology

Promoter

hPGK promoter

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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LB601: pMVP/PB/eGFP-DEST

Plasmid

#121870

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-FullLength-TET1-LLAA(20)

Plasmid

#124397

Purpose

Full-length mutant TET1 (L897A/L900A) used to PiggyBAC rescue the function of TET1/2 DKO cells

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

FLAG-FullLength-TET1 with L897A/L900A mutations (TET1 Human)

Tags

Flag

Expression

Mammalian

Mutation

L897A/L900A mutations (full length protein is 213…

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Pb-TRE3G-SpCas9-IRES-blast

Plasmid

#195506

Purpose

Inducible Piggybac vector expressing SpCas9 with Blasticidin selection

Depositor

Joanna Wysocka

Article

Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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PB-hEF1a-MG

Plasmid

#175504

Purpose

All-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elements

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Promoter

hEF1a

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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BII-Sh-FUCCI

Plasmid

#133363

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporter

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

Clover-Geminin-IRES-mKO2-Cdt1

Tags

HA-tagged Clover-Geminin

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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KA0637_pPBCAG-rtTAM2-IN

Plasmid

#124166

Purpose

piggyBac-based expression of rtTA-M2

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

rtTAM2-IRES-Neo

Expression

Mammalian

Promoter

CAG

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pB-RTP2-Integration

Plasmid

#129459

Purpose

piggyBac transposon vector that Inducibly (Tet-On) expresses RTP2

Depositor

Sriram Kosuri

Article

Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

Insert

RTP2 (RTP2 Human)

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE (Tet-On)

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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