167,729 results

1. pRosa26-EF1a-hCas9IRESneo

   Plasmid

   #67987

   Purpose

   Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo cassette

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-hCas9IRESneopA
   

   Expression

   Mammalian

   Available Since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP scFv [N86/38]

   Plasmid

   #182095

   Purpose

   Mammalian Expression of GFP scFv. Derived from hybridoma N86/38.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   recombinant mouse scFv targeting GFP
   

   Tags

   HA, His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRXIP TMEM41B-GFP

   Plasmid

   #117414

   Purpose

   Expresses TMEM41B tagged with GFP in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Morita et al J Cell Biol. 2018 Aug 9. pii: jcb.201804

   Insert

   TMEM41B (TMEM41B Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTUB1:YFP-mAID-3HA, DHFR-TS:HXGPRT

   Plasmid

   #87259

   Purpose

   YFP-mAID-3HA fusion driven by a minimal TUB1 promoter with an HXGdrug selectable marker. The mAID CDS is from Arabidopsis thaliana auxin-responsive protein IAA17E66-S133

   Depositor

   David Sibley

   Article

   Brown et al MBio. 2017 May 2;8(3). pii: e00375-17. d

   Inserts

   YFP-mAID-3HA
   HXGPRT
   

   Use

   Toxoplasma expression

   Tags

   3HA and mAID

   Mutation

   Codon optimized for Toxoplasma gondii expression

   Promoter

   TgDHFR-TS 463 bp and TgTUB1 378 bp

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. sFlt1-e15a V5

   Plasmid

   #86045

   Purpose

   human Soluble Flt1 isoform e15a (terminates in exon 15a)

   Depositor

   Christie Thomas

   Article

   Thomas et al J Clin Endocrinol Metab. 2009 Jul;94(7):

   Insert

   human soluble Flt1 isoform e15a (FLT1 Human)
   

   Tags

   6xHis and V5

   Expression

   Mammalian

   Mutation

   isoform e15a (terminates in exon 15a)

   Promoter

   CMV

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAG-dLight1.1 (AAV5)

   Viral Prep

   #111067-AAV5

   Purpose

   Ready-to-use AAV5 particles produced from pAAV-CAG-dLight1.1 (#111067). In addition to the viral particles, you will also receive purified pAAV-CAG-dLight1.1 plasmid DNA. CAG-driven expression of dLight1.1 dopamine sensor. This sensor is recommended for photometry in vivo. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Lin Tian

   Article

   Patriarchi et al Science. 2018 Jun 29;360(6396). pii: sci

   Available Since

   Dec. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

7. pBPp65ADZpUw

   Plasmid

   #26234

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   p65AD-Zip (RELA Human)
   

   Expression

   Insect

   Available Since

   Oct. 7, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pESC-TRP (V5 and HA)

   Plasmid

   #222488

   Purpose

   Co-expression of two genes in yeast with V5 and HA tags and TRP1 selection

   Depositor

   Kiyofumi Yamada, Tetsuo Matsuzaki

   Article

   Dong et al FEBS Open Bio. 2024 Oct 10. doi: 10.1002

   Type

   Empty backbone

   Tags

   HA and V5

   Expression

   Yeast

   Available Since

   Oct. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSIM24 KanR

   Plasmid

   #67583

   Purpose

   Plant Gene Expression Vector

   Depositor

   Indu Maiti

   Article

   Sahoo et al PLoS One. 2014 Jun 4;9(6):e98988. doi: 1

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   M24

   Available Since

   July 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDisplay-GRAB-gDA3h-IRES-mCherry-CAAX

    Plasmid

    #208693

    Purpose

    Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_gDA3h and a membrane-localized mcherry in mammalian cells

    Depositor

    Yulong Li

    Article

    Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    GPCR activation based dopamine (DA) sensor GRAB_gDA3h
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. DRD2-Tango

    Plasmid

    #66269

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    DRD2 (DRD2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMito-iRFP670

    Plasmid

    #45462

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP670
    

    Tags

    human cytochrome C oxidase subunit VIII

    Expression

    Mammalian

    Mutation

    T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMD2.G-K47Q/R354Q

    Plasmid

    #207323

    Purpose

    VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    VSV-G (VSIVgp4 Vesicular Stomatitis Virus)
    

    Expression

    Mammalian

    Mutation

    K47Q/R354Q

    Promoter

    CMV promoter

    Available Since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. T7RNAP Cyslight

    Plasmid

    #205077

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Article

    Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

    Insert

    T7RNAP Cyslight (T7p07 Bacteriophage T7)
    

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. myc-Rictor corrected

    Plasmid

    #11367

    Purpose

    Mammalian expression of myc tagged human RICTOR

    Depositor

    David Sabatini

    Article

    Sarbassov et al Curr Biol 2004 Jul 27;14(14):1296-302.

    Insert

    Rictor (RICTOR Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    April 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSF278

    Plasmid

    #167673

    Purpose

    Intermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    attL1-ccdB-attL2
    

    Use

    CRISPR

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWPI-LUC7L2-3xFLAG

    Plasmid

    #201641

    Purpose

    Over-expression of 3xFLAG-tagged human LUC7L2

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    LUC7L2 (LUC7L2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Available Since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Synapsin-cyto-iATPSnFR1.0

    Plasmid

    #102556

    Purpose

    expresses cytoplasmic ATP sensor in neurons

    Depositor

    Baljit Khakh

    Article

    Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

    Insert

    iATPSnFR1.0
    

    Use

    AAV

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3 luciferase

    Plasmid

    #26612

    Depositor

    Jie Chen

    Article

    Kim et al Proc Natl Acad Sci U S A. 2000 Dec 19. 9

    Insert

    firefly luciferase
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pLVX puro TRIM21-GFP

    Plasmid

    #116941

    Purpose

    Expression of TRIM21-GFP fusion protein

    Depositor

    Gaudenz Danuser

    Article

    Park et al Nature. 2020 Feb 12. pii: 10.1038/s41586

    Insert

    TRIM21 - GFP (TRIM21 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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