178,402 results
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Plasmid#172718PurposeExpression of a fast folding GFP variant GFP plusDepositorInsertGFP plus
ExpressionBacterialAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-tdTomato (AAV2)
Viral prep#28306-AAV2PurposeReady-to-use AAV2 particles produced from pAAV-FLEX-tdTomato (#28306). In addition to the viral particles, you will also receive purified pAAV-FLEX-tdTomato plasmid DNA. CAG-driven, Cre-dependent tdTomato expression control. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagstdTomato (Cre-dependent)Available sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
AttB _EGFP
Plasmid#171597PurposeStrong EGFP fluorescence from Bxb1 landing padDepositorInsertEGFP
UseSynthetic Biology; Promoterless bxb1 dna recombin…ExpressionMammalianAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Plenti-ARE-HSVTK-luc
Plasmid#161789PurposeExpresses HSV-TK and Luciferase in an NRF2 dependent manner. Also, expresses cherry and blasticidin resistance selection markersDepositorInsertsLuciferase-T2A-HSV-TK
mCherry P2A blasticidin
UseLentiviral and LuciferaseExpressionMammalianPromoterEF1a and Murine Gclc AREAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV9)
Viral prep#87306-AAV9PurposeReady-to-use AAV9 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aAvailable sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pC1-HyPerRed-mito
Plasmid#60247PurposeMitochondria-targeted genetically encoded hydrogen peroxide indicator with red fluorescence.DepositorInsertHyPerRed-mito
TagsMitochondrial targeting signalExpressionMammalianPromoterCMVAvailable sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-Cre-P2A-dTomato (AAV8)
Viral prep#107738-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsdTomato (physically separate, not a fusion protein)Available sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVS1-VIR2
Plasmid#134745PurposeVirulence and replication helperDepositorInsertvir gene cluster
UseVir gene expressionAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-sumo-NSP12 (SARS-CoV-2)
Plasmid#159107PurposeThe SARS-CoV-2 NSP12 coding sequence was cloned into a modified pRSFDuet-1 vector (Novagen) bearing an N-terminal His6-SUMO-tag which is cleavable by the ubiquitin-like protease (ULP1).DepositorInsertNSP12
TagsHis-SUMO tagExpressionBacterialMutationNonePromoterT7Available sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
C1-F-tractin-mCherry
Plasmid#155218PurposeMonitor global F-actin distributionDepositorInsertF-tractin (aa 9-52 of rat ITPKA) fused to mCherry (Itpka Rat)
TagsmCherryExpressionMammalianAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPP085
Plasmid#158795PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-2
Tags6xHisExpressionBacterialAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCX-SpiCee
Plasmid#140836Purposemammalian expression of SpiCee, a buffer of calcium that prevents downstream signalingDepositorInsertSpiCee
TagsmRFPExpressionMammalianPromoterCAGAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRISPR-HOT_tdTomato
Plasmid#138567PurposeUniversal Cleavable Donor Plasmid for tagging with tdTomato using NHEJDepositorInserttdTomato
UseUnspecifiedAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE
Plasmid#139090PurposeExpresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertdSpCas9-VPR
UseCRISPR and LentiviralExpressionMammalianMutationD10A, H840AAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro
Plasmid#132771PurposeInteracts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.DepositorInsertCyclic GMP-AMP synthase (CGAS Human)
UseLentiviralTagsmCherry2ExpressionMammalianMutationE225A D227AAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-2E2-HA-mEGFP
Plasmid#129595PurposeThe encoded protein is the anti-HA frankenbody variant fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.DepositorInsertAnti-HA frankenbody variant-mEGFP (2E2-HA scFv-mEGFP)
ExpressionMammalianPromoterCMVAvailable sinceAug. 15, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQE30-His-PheRS
Plasmid#124116PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET21a-SerRS-His
Plasmid#124118PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET21a-LysRS-His
Plasmid#124114PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ARHGEF11(DHPH)-CRY2-mCherry
Plasmid#89481PurposeAlso known as optoGEF-RhoA, encodes for a fusion between CRY2(1-498)-mCherry and the DHPH domain of ARHGEF11DepositorTagsmCherryExpressionMammalianPromoterCMVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by