We narrowed to 25,526 results for: CHI

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pJK667

Plasmid

#71690

Purpose

Produces E. coli acetate kinase with an N-terminal His6 tag (H6AckA)

Depositor

T. J. Kappock

Article

Murphy et al Front Chem. 2016 May 23;4:23. doi: 10.33

Insert

acetate kinase (ackA Escherichia coli)

Tags

His6

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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SepOTSκ

Plasmid

#68291

Purpose

Phosphoserine orthogonal translation system

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

SepRS/EF-Sep, 4xtRNA-Sep-A37

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8-cerulean3

Plasmid

#99358

Purpose

Bacterial expression of Cerulean3 fused to the 3' of cDNA of the fission yeast tropomyosin, cdc8.

Depositor

Dan Mulvihill

Article

Brooker et al FEBS Lett. 2016 Sep;590(18):3111-21. doi

Insert

cdc8 (cdc8 Fission Yeast)

Tags

Cerulean3 and Gly3-His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pFREE2-xCas9-sgRNA

Plasmid

#179579

Purpose

Expresses xCas9 and gRNA that targets ColE1 plasmids for curing.

Depositor

Kevin Solomon

Article

Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

Insert

xCas9

Expression

Bacterial

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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SuperTopAP

Plasmid

#20334

Depositor

Connie Cepko

Article

Cho et al Development. 2006 Aug . 133(16):3167-77.

Insert

beta catenin reporter (CTNNB1 Human)

Use

Ap reporter

Tags

AP

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
tdEos-Paxillin-22

Plasmid

#57653

Purpose

Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

Insert

Paxillin

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits
RCASBP-AP (E) (CC#39)

Plasmid

#15162

Depositor

Connie Cepko

Article

Fekete et al Mol Cell Biol. 1993 Apr . 13(4):2604-13.

Insert

PLAP (ALPP Human)

Use

Retroviral; Avian expression

Available Since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pPP384

Plasmid

#158799

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-3 (D413A)

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGM-ENO2-mCherry-YFP

Plasmid

#180570

Purpose

Dual fluorescent reporter for 5' UTR activity in yeast mCherry/YFP

Depositor

Joel McManus

Article

May et al Elife. 2023 May 25;12:e69611. doi: 10.75

Type

Empty backbone

Expression

Yeast

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-pvdA WT

Plasmid

#87098

Purpose

expresses PvdA, the ornithine hydroxylase from Pseudomonas aeruginosa

Depositor

Audrey Lamb

Article

Olucha et al J Biol Chem. 2011 Sep 9;286(36):31789-98

Insert

pvdA WT

Tags

his-tag

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2017

Availability

Academic Institutions and Nonprofits only

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Cx55.5:rasGFP

Plasmid

#105939

Purpose

in vitro transcription

Depositor

Caren Norden

Article

Weber et al Cell Rep. 2014 Apr 24;7(2):386-97. doi:

Insert

Ras

Tags

GFP

Expression

Bacterial

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pZA2[PTAN:GFP]

Plasmid

#60779

Purpose

Contains PTAN driving expression of GFP

Depositor

Matthew Bennett

Article

Shis et al ACS Synth Biol. 2014 Sep 19;3(9):645-51.

Insert

yemGFP

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pGM-ENO2-YFP-mCherry

Plasmid

#180569

Purpose

Dual fluorescent reporter for 5' UTR activity in yeast YFP/mCherry

Depositor

Joel McManus

Article

May et al Elife. 2023 May 25;12:e69611. doi: 10.75

Type

Empty backbone

Expression

Yeast

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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pC0.286

Plasmid

#119627

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

AbRChl

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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f2H ∂HD (272)

Plasmid

#24428

Depositor

Steven Rosen

Article

Morimoto-Tomita et al Neoplasia. 2005 Nov . 7(11):1001-10.

Insert

SULF2 (SULF2 Human)

Tags

FLAG, His, and Myc

Expression

Mammalian

Mutation

AA 418-716 deleted, 23AA N-terminal truncation

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
CMV-dH3-YFP

Plasmid

#8694

Depositor

Kami Ahmad

Article

Janicki et al Cell 2004 Mar 5;116(5):683-98.

Insert

H3 (His3:CG31613 Fly)

Tags

YFP

Expression

Mammalian

Available Since

June 30, 2005

Availability

Academic Institutions and Nonprofits only

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pLEX_307_ms2_VP64

Plasmid

#79375

Purpose

Recruits VP64 to compatible gRNAs for transcriptional activation

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

ms2-VP64

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pY1

Plasmid

#50597

Purpose

Backbone: pRBS-01, Origin Of Replication: p15a, Selection Markers: Amp, Promoters: PLacUV5,

Depositor

Jay Keasling

Article

Juminaga et al Appl Environ Microbiol. 2012 Jan;78(1):8

Type

Empty backbone

Use

Synthetic Biology; Empty vector

Expression

Bacterial

Promoter

PLacUV5

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only
P3U2C5T2-v1-2 (Pl-lacO1-tetR) (136p)

Plasmid

#70212

Purpose

1 Part of 4-part switch

Depositor

Richard Murray

Article

Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

Insert

tetR

Available Since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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mEGFP-30nmSAH-GFP(1-10)

Plasmid

#61677

Purpose

GFP-labeled SAH scaffold with split GFP attachment site

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

mEGFP-30nmSAH-GFP(1-10)

Available Since

March 19, 2015

Availability

Academic Institutions and Nonprofits only

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