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pLV-CMV-XRCC1-EGFP-Hygro

Plasmid

#176062

Purpose

EGFP fused to the C-terminus of XRCC1 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

X-ray repair cross complementing 1 (XRCC1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti_Efs_hSpCas9_NLS_FLAG-WPRE

Plasmid

#97313

Purpose

Lentiviral vector for encoding a human codon-optimized SpCas9 driven by EFs promoter.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

humanized S. pyogenes Cas9

Use

Lentiviral and Mouse Targeting

Tags

Flag

Expression

Mammalian

Promoter

EFS

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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dCas9-GCN5 FL

Plasmid

#179552

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter

Depositor

Isaac Hilton

Article

Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

Insert

S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)

Use

Lentiviral

Tags

Flag Tag

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

EF-1alpha

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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single polypeptide FPX biosensor for caspase-8

Plasmid

#60884

Purpose

FPX biosensor for caspase-8

Depositor

Robert Campbell

Article

Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

Insert

single polypeptide FPX biosensor for caspase-8

Tags

HindIII site and NES sequence LALKLAGLDIGS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2015

Availability

Academic Institutions and Nonprofits only
pLenti-sgAMPKa1-Cas9-GFP

Plasmid

#208049

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting AMPKa1

Depositor

Naama Kanarek

Article

Maynard et al Sci Adv. 2024 Feb 2;10(5):eadj9479. doi:

Insert

sgPRKAA1 (PRKAA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUC57-sgRNA-MS2-U6

Plasmid

#171694

Purpose

To construct expression vector for tBE-V5-mA3

Depositor

Jia Chen

Article

Wang et al Nat Cell Biol. 2021 May 10. pii: 10.1038

Insert

sgRNA scaffold-MS2-U6

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 9, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-GFAP-TurboID

Plasmid

#227179

Purpose

Co-expression of FLAG-tagged-TurboID and T2A-mCherry under the GFAP short promoter by AAV vector

Depositor

Junmin Peng

Article

Sun et al Anal Chem. 2022 Apr 5;94(13):5325-5334.

Insert

TurboID

Use

AAV

Tags

FLAG and T2A-mCherry

Expression

Mammalian

Promoter

GFAP_short

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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Chr7_Centromere-Targeting_gRNA

Plasmid

#195129

Purpose

dual gRNA vector targeting centromere-proximal locations on Chromosome 7p and 7q in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr7 gRNA

Expression

Mammalian

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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px551-mSncg-0.27kb-HA-SpCas9

Plasmid

#153208

Purpose

Truncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cells

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Insert

Cas9

Use

AAV and CRISPR

Tags

HA

Expression

Mammalian

Promoter

Truncated mSncg (0.27kb)

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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JAM2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176966

Purpose

Mammalian expression vector encoding JAM2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

JAM2 (JAM2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pINDUCER12 (miR-RUL)

Plasmid

#46935

Purpose

Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI to EcoRI;1.4kb),can be replaced w mir30 based hairpin of interest

Depositor

Thomas Westbrook

Article

Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

Insert

PheS Gly294

Use

Lentiviral

Available Since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC9-C169S

Plasmid

#231673

Purpose

Expresses human ZDHHC9-C169S protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC9 (ZDHHC9 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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pJS647

Plasmid

#183176

Purpose

PYR1 mutagenesis plasmid compatible with pJS646 Y2H vector

Depositor

Timothy Whitehead

Article

Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

Insert

PYR1 (PYR1 Mustard Weed)

Expression

Bacterial

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgAMPKa1

Plasmid

#138684

Purpose

Expresses a human AMPKa1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgAMPKa1 human (PRKAA1 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_FH-AGO2-5xA

Plasmid

#92007

Purpose

Expresses FLAG-HA-AGO2 [S824A, S828A, T830A, S831A, S834A (5xA)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824A, S828A, T830A, S831A, S834A (5xA)

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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FUG-T2A-Pten

Plasmid

#140738

Purpose

Lentiviral vector to overexpress human PTEN and GFP

Depositor

Bryan Luikart

Article

Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

Insert

GFP-PTA-PTEN (PTEN Human)

Use

Lentiviral

Tags

none

Expression

Mammalian

Promoter

hUbiC

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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LZRS-Flag_EZH2_ER

Plasmid

#26111

Depositor

Howard Chang

Article

Gupta et al Nature. 2010 Apr 15. 464(7291):1071-6.

Insert

EZH2 (EZH2 Human)

Use

Retroviral

Tags

ER and FLAG

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pCFJ1320

Plasmid

#84829

Purpose

Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 introns

Depositor

Andrew Fire

Article

Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

Insert

C. elegans codon optimized GFP

Expression

Bacterial and Worm

Promoter

Peft-3

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-hAID-BE3

Plasmid

#113417

Purpose

Expresses hAID-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hAID-BE3 (AICDA Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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AAV_Cdx2 HMEJ donor

Plasmid

#97319

Purpose

HMEJ donor for fusing a p2A-mCherry reporter to mouse Cdx2. AAV backbone.

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

Cdx2 HMEJ donor

Use

AAV and Mouse Targeting

Expression

Mammalian

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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