161,697 results

1. p2T-U6-sgGAA

   Plasmid

   #232724

   Purpose

   Cas9 guide RNA targeting GAA repeats for A-to-G base editing

   Depositor

   David Liu

   Article

   Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

   Insert

   SpCas9 gRNA targeting GAA repeats
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Orai1-jGCaMP8f V203Y

   Plasmid

   #231631

   Purpose

   Green fluorescent reporter of Orai1-associated calcium influx

   Depositor

   Michael Cahalan

   Article

   Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

   Insert

   Orai1-jGCaMP8f V203Y (ORAI1 Synthetic, Human)
   

   Use

   Tags

   jGCaMP8f V203Y

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Immediate Early

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. BALF5-WT

   Plasmid

   #192454

   Purpose

   Expresses wild-type BALF5 (codon-optimized)

   Depositor

   Bill Sugden

   Article

   Rosemarie et al mBio. 2023 Feb 28;14(1):e0268622. doi: 1

   Insert

   BALF5 (WT) (BALF5 Epstein-Barr Virus)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. PDG459 V3

   Plasmid

   #226958

   Purpose

   CBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.

   Depositor

   Paul Thomas

   Article

   Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. GB4542

   Plasmid

   #215261

   Purpose

   Module for the expression of the N. nambi HISPS gene under the regulation of the copper inducible CBS4x:mDFR promoter and TNOS and the constitutve expression of CPH, LUZ, H3H, EGFP and p19

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   CBS4x:mDFR:HISPS-SF-CPH-SF-LUZ-H3H-EGFP-p19
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AiP15050 - pAAV-AiE1426m-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN5050)

   Plasmid

   #224192

   Purpose

   AiE1426m is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal Sst_Chodl neurons

   Depositor

   The Allen Institute for Brain Science, Jonathan Ting

   Article

   Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

   Insert

   SYFP2-P2A-3XFLAG-10aa-H2B
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. GB4401

   Plasmid

   #215252

   Purpose

   Module for the expression of the N. nambi HISPS gene under the regulation of PNOS and TNOS and the constitutive expression of CPH, LUZ, H3H, EGFP and p19.

   Depositor

   Diego Orzaez

   Article

   Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

   Insert

   SF-PNOS:HISPS:TNOS-CPH-LUZ-H3H-EGFP-p19
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. MDC1 KO sgRNA

   Plasmid

   #207103

   Purpose

   pX330 based plasmid for expression of Cas9 and the GGTGTAACGTGGAGCCAGTA sgRNA to target the MDC1 coding sequence.

   Depositor

   Jens Schmidt

   Article

   Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

   Insert

   GGTGTAACGTGGAGCCAGTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PET-B-evoSpCas9-NLS-6xHis

   Plasmid

   #207390

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

   Promoter

   T7

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. A*24:07:01

    Plasmid

    #203283

    Purpose

    Overexpress HLA allele

    Depositor

    Derin Keskin, Catherine Wu

    Article

    Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

    Insert

    A*24:07:01 (HLA-A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBbi-NLSmCherryNLS Clover-HDHB

    Plasmid

    #190223

    Purpose

    Fluorescent cell cycle reporter

    Depositor

    Laura Heiser

    Article

    Gross et al Nat Commun. 2023 Jun 10;14(1):3450. doi:

    Inserts

    Human DNA Helicase B (amino acids 994-1087) (HELB Human)
    NLS-mCherry-NLS-P2A-Puromycin
    

    Use

    Transposon

    Tags

    Clover fluorescent protein

    Expression

    Mammalian

    Mutation

    Amino Acids 994-1087

    Promoter

    EF1a and RPBSA

    Available since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiGuide_mCherry_NT135

    Plasmid

    #183121

    Purpose

    pLentiGuide modified to express mCherry and a non-targeting sgRNA

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    NT135
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. AiP993-Ai195 (TIT2L-GC7s-ICF-IRES-tTA2)

    Plasmid

    #181762

    Purpose

    targeting vector

    Depositor

    Tanya Daigle

    Insert

    GCaMP7s and tTA2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RABEP1A-c019

    Plasmid

    #175477

    Purpose

    Protein expression in insect cells. Entire CDS, M1-T862. Codon optimised.

    Depositor

    Opher Gileadi

    Insert

    RABEP1 (RABEP1 Human)
    

    Use

    Tags

    TEV-TwinStrep

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. SNX32A-c014

    Plasmid

    #175486

    Purpose

    Protein expression in bacterial cells. PX domain, S17-M197

    Depositor

    Opher Gileadi

    Insert

    SNX32 (SNX32 Human)
    

    Use

    Tags

    His6-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLfa-PUb-EGFP-3'UTR#13

    Plasmid

    #176338

    Purpose

    The synthetic 3'UTR#13 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoter

    Depositor

    Zach Adelman

    Article

    Chae et al RNA Biol. 2021 Aug 31:1-9. doi: 10.1080/

    Insert

    3'UTR#13
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1(+)_Granulin 4-no linker

    Plasmid

    #176925

    Purpose

    express Granulin 4 without linker 5 in mammalian cells

    Depositor

    Thomas Kukar

    Article

    Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

    Insert

    Granulin 4 (GRN Human)
    

    Use

    Tags

    Twin-Strep and FLAG tags encoded after Signal Pep…

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAT15068-evoCBE

    Plasmid

    #163013

    Purpose

    plasmid expressing evoCBE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    evoCBE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEPQD0CM0296

    Plasmid

    #162305

    Purpose

    Level 0 CDS part for MoClo assembly, AATG-TTCG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    CDS, superfolder GFP, no stop codon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-P4:gs:nls:CIBN

    Plasmid

    #135986

    Purpose

    Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells

    Depositor

    Roman Jerala

    Article

    Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

    Insert

    P4 coiled-coil fused to the CIBN domain
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge