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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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161,697 results

Showing: 8401 - 8420 of 161697 results

  1. pSynSRE-T-Luc

    Plasmid
    #60444
    Purpose
    Uses the hamster HMG-CoA synthase promoter sequence (-324/-225) and minimal HMG-CoA sythnase TATA box (-28/+39) to study SREBP-dependent transcriptional activation.
    Depositor
    Timothy Osborne
    Article
    Dooley et al J Biol Chem. 1998 Jan 16;273(3):1349-56.
    Insert
    HMG-CoA synthase promoter (-324/-225)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    HMG-CoA synthase (-324/-225) & TATAA (-28/+39)
    Available since
    Feb. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    60444-plasmid-map-sequence-id-266122

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  3. ARHGEF2A-c055

    Plasmid
    #205103
    Purpose
    ARHGEF2 protein expression
    Depositor
    Paul Brennan
    Insert
    ARHGEF2 (ARHGEF2 Human)
    Use
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205103-plasmid-map-sequence-id-412139

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  4. ARHGEF2A-c052

    Plasmid
    #205102
    Purpose
    ARHGEF2 protein expression
    Depositor
    Paul Brennan
    Insert
    ARHGEF2 (ARHGEF2 Human)
    Use
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205102-plasmid-map-sequence-id-412138

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  5. RHOAA-c035

    Plasmid
    #205118
    Purpose
    RHOA protein expression
    Depositor
    Paul Brennan
    Insert
    RHOA (RHOA Human)
    Use
    Tags
    6His-TEV and AviTag
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    205118-plasmid-map-sequence-id-411209

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  8. TFORF0282

    Plasmid
    #141561
    Purpose
    Lentiviral vector for overexpressing the MEF2C transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    MEF2C (MEF2C Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141561-plasmid-map-sequence-id-276819

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  12. 24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

    Plasmid
    #128605
    Purpose
    Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.
    Depositor
    Marvin Tanenbaum
    Article
    Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304
    Inserts
    24xMoonTag
    kinesin family member 18b (KIF18B Human)
    24xSunTag
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Pcmv
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    128605-plasmid-map-sequence-id-318004

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  14. pCAG-GFP-CD63

    Plasmid
    #194989
    Purpose
    overexpress CD63
    Depositor
    Angelique Bordey
    Insert
    GFP-CD63
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    194989-plasmid-map-sequence-id-387085

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  15. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

    Plasmid
    #213026
    Purpose
    Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2
    Depositor
    Martin S. Taylor
    Article
    Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:
    Insert
    Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
    Use
    Tags
    3xFlag (ORF2p)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    213026-plasmid-map-sequence-id-422069

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  16. pRecT_2

    Plasmid
    #167546
    Purpose
    Encodes an IPTG inducible recT from E. faecium Com15 used to ectopically express recT in Enterococcus for recombineering.
    Depositor
    Howard Hang
    Article
    Chen et al Appl Environ Microbiol. 2021 Jul 7:AEM00
    Insert
    E. faecium Com15 recT under an IPTG inducible promoter, with lacI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    IPTG
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    167546-plasmid-map-sequence-id-332204

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Showing: 8401 - 8420 of 161697 results