161,697 results

1. pcDNA3.1(+)-PM-RasAR

   Plasmid

   #205569

   Purpose

   Plasma membrane-targeted RasAR.

   Depositor

   Jin Zhang

   Article

   Weeks et al J Am Chem Soc. 2022 Sep 28;144(38):17432

   Insert

   PM-RasAR (RAF1 Synthetic, Human)
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pmal_c5x_RNAse_Inhib

   Plasmid

   #153314

   Purpose

   Bacterial expression of an RNase inhibitor

   Depositor

   Drew Endy, Philippa Marrack

   Insert

   RNAse Inhibitor
   

   Use

   Tags

   Maltose Binding Protein

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pCDNA 3.1 Flag-rat-PTP1B

   Plasmid

   #35988

   Depositor

   Anna Huttenlocher

   Article

   Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

   Insert

   protein tyrosine phosphatase 1B (Ptpn1 Rat)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGP-pcDNA3.1 Puro-CAG-Voltron2

   Plasmid

   #172909

   Purpose

   Mammalian expression of voltage sensor

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Voltron2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TFORF1922

   Plasmid

   #141888

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZBTB20 (ZBTB20 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TFORF0980

   Plasmid

   #141777

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   KLF7 (KLF7 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV CTG 700x

   Plasmid

   #63087

   Purpose

   AdenoAssociated Virus plasmid containing expanded repeats--CTG 700x that is the mutation found in Myotonic Dystrophic type 1

   Depositor

   Nicolas Charlet-Berguerand

   Insert

   CTG REPEATS
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   CTG 700x that is the mutation found in Myotonic D…

   Promoter

   CMV

   Available since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mCherry-DFCP1

   Plasmid

   #86746

   Purpose

   N-terminal mCherry-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

   Insert

   human DFCP1 (ZFYVE1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTP955

   Plasmid

   #104164

   Purpose

   Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)
   

   Use

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCDNA3 Flag Jnk2a1

    Plasmid

    #13754

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk2a1 (MAPK9 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

11. AID∆C-Cas9n-UGI-NLS

    Plasmid

    #198890

    Purpose

    Express AID(1-188) BE3-based base editor

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    Actication induced deaminase (AICDA Human)
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 189-198

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Mito-TurboID

    Plasmid

    #200960

    Purpose

    Transiently expresses mitochondria localized TurboID with V5 tags

    Depositor

    Keriann Backus

    Article

    Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

    Insert

    mito-TurboID
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. METTL7A pEGFP-N2 (890)

    Plasmid

    #62017

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

    Insert

    METTL7A (METTL7A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

14. MSCV-N-Flag-HA-IRES-PURO

    Plasmid

    #41033

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Type

    Empty backbone

    Use

    Retroviral; Gateway destination vector

    Tags

    Flag, HA, and IRES Puro

    Expression

    Mammalian

    Mutation

    Promoter

    LTR-driven expression

    Available since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ii-Str_SBP-EGFP-Ecadherin

    Plasmid

    #65288

    Purpose

    synchronize trafficking of Ecadherin from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCOLA-Gent-EM7-Erv1p-PDI

    Plasmid

    #202486

    Purpose

    Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    EM7

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-FLAG-DDB1

    Plasmid

    #19918

    Depositor

    Yue Xiong

    Article

    Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

    Insert

    DDB1 (Damage-specific DNA binding protein 1) (DDB1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-ABEmax-SpRY

    Plasmid

    #195279

    Purpose

    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP

    Depositor

    Carsten Lederer, Petros Patsali

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pmScarlet_peroxisome_C1

    Plasmid

    #85063

    Purpose

    In vivo visualization of the peroxisome (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Peroxisomal targeting sequence
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMVHA hEZH2

    Plasmid

    #24230

    Purpose

    Mammalian expression of human EZH2 with HA tag

    Depositor

    Kristian Helin

    Article

    Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge