We narrowed to 8,725 results for: PAN
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Plasmid#194234PurposeLentiviral expression vector expressing Jason McClellan lab's 6-proline Spike extracellular domain on the cell surface attached to PDGFR's transmembrane domain and mCherry transduction markerDepositorAvailable SinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCOA-Leu-FvPPT1-fsr1
Plasmid#179868PurposeConstitutive expression vector for Saccharomyces cerevisiae comprising the polyketide synthase fsr1 from Fusarium vanettenii and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1DepositorInsertsPPT1
fsr1
ExpressionYeastMutationCodon optimized for expression in Saccharomyces c…PromoterYarrowia lipolytica EXP1 and Yarrowia lipolytica …Available SinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Shank3 scFv [N69/46]
Plasmid#206727PurposeMammalian Expression of Shank3 scaffold protein scFV. Derived from hybridoma N69/46 scFv.DepositorInsertShank3 scaffold protein (Rattus norvegicus) recombinant scFV (Shank3 Mouse)
ExpressionMammalianPromoterCMVAvailable SinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Shank1 scFv [N22/21]
Plasmid#190539PurposeMammalian Expression of Shank1 scFV. Derived from hybridoma N22/21.DepositorInsertShank1 (Rattus norvegicus) recombinant scFV (Shank1 Mouse)
TagsHA, Sortase, 6xHisExpressionMammalianPromoterCMVAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Shank2 scFv [N23B/6]
Plasmid#190543PurposeMammalian Expression of Shank2 scFV. Derived from hybridoma N23B/6.DepositorInsertShank2 (Rattus norvegicus) recombinant scFV (Shank2 Mouse)
TagsHA, Sortase, 6xHisExpressionMammalianPromoterCMVAvailable SinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
M3K5_HUMAN_D0
Plasmid#79710PurposeThis plasmid encodes the kinase domain of M3K5. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
E2AK2_HUMAN_D0
Plasmid#79712PurposeThis plasmid encodes the kinase domain of E2AK2. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DMPK_HUMAN_D0
Plasmid#79717PurposeThis plasmid encodes the kinase domain of DMPK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
KC1G1_HUMAN_D0
Plasmid#79691PurposeThis plasmid encodes the kinase domain of KC1G1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSK22_HUMAN_D0
Plasmid#79704PurposeThis plasmid encodes the kinase domain of CSK22. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pAzF[TAG]
Plasmid#164579PurposeMachinery plasmid containing two copies of the Mj pCNFRS and cognate tRNA for incorporation of pAzF, pCNF, or pENF at TAG codonsDepositorInsertsMj pCNFRS[TAG] (aaRS1)
Mj pCNFRS[TAG] (aaRS2)
Mj tRNA[TAG]
ExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoteraraBAD, glnS, and proKAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pERM2-nhpSer
Plasmid#201922PurposeEnables translational incorporation of non-hydrolyzable phosphoserine into proteins at TAG codons in E coli. Do not use for phosphoserine incorporation.DepositorInsertsSepRS(2)
Sep-tRNA v2.0
SerB
EF-Sep
TagsNoneExpressionBacterialMutationE412P, E414F, T417K, P495M, I496W, F529S and H67R…PromoterGlnS (constitutive), OXB20 (constitutive), lpp (c…Available SinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET01-TAF5-ex8-Minigene-SRSF1 motif MUT
Plasmid#218973PurposeVector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)DepositorInsertTAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
ExpressionMammalianMutationmutant SRSF1 binding motif (AGTCTCCT)PromoterRSV LTRAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)
Plasmid#182652PurposeEncodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
4xU6-tRNAM15
Tagsnuclear export signal (NES)ExpressionMammalianMutationY306A/Y384F (AF) double mutant of Methanosarcina …PromoterCMV and U6Available SinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-PHFF-sh-hBVRA
Plasmid#100285PurposeExpression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cellsDepositorInsertsMTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
shRNA for human BVRA
TagsFLAG, T7 and Mitochondria-targeting sequence (MTS…ExpressionMammalianMutationAll genes are codon-optimized for expression in h…PromoterCAG promoter and H1 promoterAvailable SinceSept. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
ABL1_HUMAN_D0
Plasmid#79727PurposeThis plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (A7)
Plasmid#174079PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70T D158H I159A L162RPromoterGlnRS (constitutive)Available SinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-Ma-Acridone-RS1 (A7)
Plasmid#197566PurposeExpression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cellsDepositorInsertsM. alvus PylRS - Acd RS1 (A7)
M. alvus Pyl-tRNA (6) (4x copies)
TagsNoneExpressionMammalianMutationN166A, V168G, W239CPromoterCMV and U6/H1Available SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTetON-DHFRdd-SpvB; CMV-mCherry
Plasmid#89463PurposeTet/Dox-inducible expression of DHRFdd-SpvB (Salmonella SpvB 375-591) plus constitutive expression of mCherry in mammalian cells (Dual-promoter)DepositorInsertDeAct-SpvB (spvB Salmonella enterica)
TagsE. coli DHFRdd destabilization domain and mCherry…ExpressionMammalianMutationTruncation spanning SpvB amino acids 375-591 (mon…PromoterTRE3GAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDule-3-nitroTyrosine (A7)
Plasmid#174078PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70T D158H I159A L162RPromoterGlnRS (constitutive)Available SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only