161,697 results

1. pCherry.90 beta

   Plasmid

   #108223

   Purpose

   Mammalian expression vector for mCherry fusion to human Hsp90 beta

   Depositor

   Didier Picard

   Article

   Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

   Insert

   mCherry-Hsp90b (HSP90AB1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQE-60NA-msGFP2

   Plasmid

   #135301

   Purpose

   Encodes a photostable monomeric superfolder GFP

   Depositor

   Benjamin Glick

   Article

   Valbuena et al Traffic. 2020 May 15. doi: 10.1111/tra.1

   Insert

   msGFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF1447

   Plasmid

   #143278

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NR5A2 (NR5A2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. ARHGEF2A-c055

   Plasmid

   #205103

   Purpose

   ARHGEF2 protein expression

   Depositor

   Paul Brennan

   Insert

   ARHGEF2 (ARHGEF2 Human)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. ARHGEF2A-c052

   Plasmid

   #205102

   Purpose

   ARHGEF2 protein expression

   Depositor

   Paul Brennan

   Insert

   ARHGEF2 (ARHGEF2 Human)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. RHOAA-c035

   Plasmid

   #205118

   Purpose

   RHOA protein expression

   Depositor

   Paul Brennan

   Insert

   RHOA (RHOA Human)
   

   Use

   Tags

   6His-TEV and AviTag

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pQE30-His-IF2

   Plasmid

   #124124

   Purpose

   Bacterial expression plasmid for PURE system components

   Depositor

   Sebastian Maerkl, Takuya Ueda

   Article

   Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

   Insert

   Initiation factor 2 (infB Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiCas9n(D10A)-Blast

   Plasmid

   #63593

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9n (D10A nickase) protein and blasticidin resistance from EFS promoter. Lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, nickase mutant

   Promoter

   EFS-NS

   Available since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRIPZ shSTING-Hsa

   Plasmid

   #128158

   Purpose

   Doxycyclin inducible shRNA knockdown of human STING gene

   Depositor

   Sandra Demaria

   Article

   Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

   Insert

   shSTING Hsa (STING1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. PSL1180polyUBdsRED

    Plasmid

    #49327

    Purpose

    Donor plasmid for homologous recombination that expresses dsRED constitutively using the Aedes aegypti polyubiquitin (PUb) promoter.

    Depositor

    Leslie Vosshall

    Insert

    dsRED
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Aedes polyubiquitin

    Available since

    Nov. 18, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. 4xmts-mNeonGreen

    Plasmid

    #98876

    Purpose

    In vivo visualization of mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv 160374

    Insert

    4xmts
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pME-mVenus-CAAX

    Plasmid

    #75153

    Purpose

    Membrane targeted mVenus (yellow) fluorophore for use with 5’ promoters.

    Depositor

    Nicholas Cole

    Article

    Don et al Zebrafish. 2016 Sep 15.

    Insert

    mVenus-CAAX
    

    Use

    Middle entry vector for multisite gateway three f…

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG:GPI-GFP

    Plasmid

    #32601

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Rhee et al Genesis. 2006 Apr;44(4):202-18.

    Insert

    GPI-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Mtp-p53-ECFP

    Plasmid

    #53961

    Purpose

    Expresses CFP tagged p53 in mammalian cells

    Depositor

    Uri Alon, Galit Lahav

    Article

    Lahav et al Nat Genet. 2004 Feb;36(2):147-50. Epub 2

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    metallothionein promoter

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. MS2-epegRNA-Dnmt1

    Plasmid

    #211372

    Purpose

    Dnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP production

    Depositor

    David Liu

    Article

    An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

    Insert

    MS2-epegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTriExRhoA2G

    Plasmid

    #40176

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    RhoA Biosensor
    

    Use

    Tags

    His-Myc

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

    Plasmid

    #207878

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJRH029

    Plasmid

    #171060

    Purpose

    Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Insert

    Gag-spyCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    2x SV40 NLS and 3x FLAG tag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-Roxed-Cre

    Plasmid

    #51273

    Purpose

    Component of the Co-Driver binary site-specific recombinase system, generates functional rox60-71Cre upon removal of an internal STOP cassette by Dre

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    Roxed-Cre
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEF1a-mMLH1dn

    Plasmid

    #174825

    Purpose

    Mammalian expression of mouse MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    mouse MLH1dn (codon optimized)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deletion of residues 758-760

    Promoter

    EF1a

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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