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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 42,817 results for: LAS

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Showing: 8681 - 8700 of 42817 results

  1. pT7-V5-SBP-C1-HsRRP6

    Plasmid
    #64916
    Purpose
    expresses V5- and SBP-tagged RRP6 in mammalian cells
    Depositor
    Elisa Izaurralde
    Insert
    RRP6 (EXOSC10 Human)
    Tags
    V5 and SBP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    64916-plasmid-map-sequence-id-109664

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  3. AMA1-bio

    Plasmid
    #47741
    Purpose
    Expresses enzymatically monobiotinylated full-length AMA1 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised AMA1
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47741-plasmid-map-sequence-id-68749

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  8. RHOA

    Plasmid
    #73231
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RHOA (RHOA Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00071:Ras 7-180
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73231-plasmid-map-sequence-id-136222

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  11. pRL_GI_PCB28

    Plasmid
    #98745
    Purpose
    For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919
    Inserts
    heme oxygenase 1 (TED4 Mustard Weed)
    Phycocyanobilin:ferredoxin oxidoreductase
    Use
    Synthetic Biology
    Expression
    Yeast
    Mutation
    Codon optimized for Saccharomyces cerevisiae
    Promoter
    ADH1m and PGK1
    Available Since
    Aug. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98745-plasmid-map-sequence-id-189854

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  12. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Available Since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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  13. BRCA1-plvxt

    Plasmid
    #119281
    Purpose
    Doxycycline-inducible human BRCA1
    Depositor
    Joan Massague
    Insert
    BRCA1 (BRCA1 Human)
    Expression
    Mammalian
    Promoter
    TRE
    Available Since
    Jan. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    119281-plasmid-map-sequence-id-237541

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  15. p663-UBC-TurboID-V5-eGFP_IDG-K

    Plasmid
    #135264
    Purpose
    Gateway destination clone of eGFP (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    TurboID-V5-eGFP
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Promoter
    Ubiquitin
    Available Since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    135264-plasmid-map-sequence-id-268064

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  18. TOPOII Pdx1 (DM#260)

    Plasmid
    #15025
    Depositor
    Douglas Melton
    Insert
    Pdx1 (Pdx1 Mouse)
    Expression
    Bacterial
    Available Since
    Oct. 18, 2007
    Availability
    Academic Institutions and Nonprofits only

  20. FUGW-APPwt-T2A-mCherry

    Plasmid
    #190803
    Purpose
    Express wild-type APP from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APP (APP Human)
    Use
    Lentiviral
    Promoter
    Ubi C
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190803-plasmid-map-sequence-id-374464

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Showing: 8681 - 8700 of 42817 results