We narrowed to 16,272 results for: GRN

1. pLenti-Cas9-NS-B

   Plasmid

   #207824

   Purpose

   Dual expression of Cas9 and non-specific sgRNA

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   non-specific sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Promoter

   EF-1a; U6

   Available Since

   Dec. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pML45

   Plasmid

   #206995

   Purpose

   Contains gRNA targeting mouse Ptbp1 gene and Cas9 ORF.

   Depositor

   Eugene Makeyev

   Article

   Kainov et al STAR Protoc. 2023 Oct 18;4(4):102644. do

   Insert

   Ptbp1 gRNA (Ptbp1 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRANS-Pho2-1/2-2-Csy4

   Plasmid

   #161763

   Purpose

   pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x Csy4-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)

   Depositor

   Shaun Curtin

   Article

   Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

   Insert

   Cas9 and gRNAs
   

   Expression

   Plant

   Promoter

   CmYLCV Promoter

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRANS-Pho2-1/2-2-tRNA

   Plasmid

   #161762

   Purpose

   pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)

   Depositor

   Shaun Curtin

   Article

   Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

   Insert

   Cas9 and gRNAs
   

   Expression

   Plant

   Promoter

   CmYLCV Promoter

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSCL.178

   Plasmid

   #184999

   Purpose

   Express -Eco1 FANCF editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: FANCF targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   FANCF donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSCL.176

   Plasmid

   #184997

   Purpose

   Express -Eco1 RNF2 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: RNF2 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   RNF2 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSCL.179

   Plasmid

   #185000

   Purpose

   Express -Eco1 HEK4 editing ncRNA and gRNA

   Depositor

   Seth Shipman

   Article

   Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

   Insert

   Eco1: HEK4 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
   

   Tags

   SV40NLS

   Expression

   Mammalian

   Mutation

   HEK4 donor, a1/a2 length extended for 27 bp

   Promoter

   H1

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLiP-G1G2

   Plasmid

   #188965

   Purpose

   L-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdown

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   dCas9
   sgRNA 1: atcggtcgcattgttttccactagg, sgRNA 2: gttagacgctgattacatggactagg
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PrhaBAD

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTW045

   Plasmid

   #185690

   Purpose

   T-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAs

   Depositor

   Feng Zhang

   Article

   Weiss et al Plant J. 2020 Nov;104(3):828-838. doi: 1

   Insert

   Cas9, Drm1b gRNAs
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTW037

    Plasmid

    #185688

    Purpose

    T-DNA for creating transgenic plants expressing Cas9 and Drm1b gRNAs

    Depositor

    Feng Zhang

    Article

    Weiss et al Plant J. 2020 Nov;104(3):828-838. doi: 1

    Insert

    Cas9, Drm1b gRNA
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSC51

    Plasmid

    #104825

    Purpose

    CRISPR/Cas9 2xplex gRNA targeting Glyma.12g075700, Glyma.11g145900 (Drb2ab). Also expresses Cas9 from rolD promoter from Gmubi promoter

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Glyma.12g075700, Glyma.11g145900
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pROS13_IDH2 #7

    Plasmid

    #166102

    Purpose

    This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.

    Depositor

    Matthias Heinemann

    Article

    Chen et al bioRxiv

    Insert

    IDH2 sgRNA #7 (IDH2 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pROS13_IDH1 #4

    Plasmid

    #166101

    Purpose

    This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.

    Depositor

    Matthias Heinemann

    Article

    Chen et al bioRxiv

    Insert

    IDH1 sgRNA #4 (IDH1 Budding Yeast)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO-Cre sgUroD v4

    Plasmid

    #158036

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse UroD

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    UroD sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pLKO-Cre sgItgb5 v3

    Plasmid

    #158038

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Itgb5

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Itgb5 sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 30, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pLKO-Cre sgRipk4 v3

    Plasmid

    #158035

    Purpose

    lenti-viral construct with Cre recombinase and U6 driven sgRNA against mouse Ripk4

    Depositor

    Daniel Schramek

    Article

    Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

    Insert

    Ripk4 sgRNA
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. eSpCas9(1.1)-Cdt1

    Plasmid

    #122342

    Purpose

    Expresses sgRNA targeting mouse Cdt1 and eSpCas9 in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Cdt1
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas9n-Nanog-L

    Plasmid

    #122302

    Purpose

    Expresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cells

    Depositor

    Hiroshi Ochiai

    Article

    Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

    Insert

    sgRNA for mouse Nanog (Nanog Synthetic)
    

    Expression

    Mammalian

    Available Since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP

    Plasmid

    #121509

    Purpose

    Expresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgFah.intron9
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAVsc.U6-sgIdua.CMV/CB-EGFP

    Plasmid

    #121511

    Purpose

    Expresses sgRNA targeting mouse Idua intron 9 (sgIdua).

    Depositor

    Guangping Gao

    Article

    Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

    Insert

    sgIdua
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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