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Viral prep#104061-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV-CAG-NLS-GFP (#104061). In addition to the viral particles, you will also receive purified pAAV-CAG-NLS-GFP plasmid DNA. CAG-driven NLS-GFP expression. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsGFPAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only
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SepOTSλ
Plasmid#68292PurposePhosphoserine orthogonal translation system (preferred variant)DepositorInsertSepRS9/EFSep21, 4xtRNA-Sep-A37
UseSynthetic BiologyExpressionBacterialAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP VAMP7 (1-220)
Plasmid#42316DepositorAvailable sinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3 myc CIITA (P#808)
Plasmid#14650DepositorPublicationAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-EGFP-Rac1(T17N)
Plasmid#13721DepositorAvailable sinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Cdc2-DN-HA
Plasmid#1889DepositorAvailable sinceFeb. 14, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lenti-SpCas9 hygro
Plasmid#104995PurposeThis lentiviral construct delivers hSpCas9 and hygromycin resistance.DepositorInsertKpnI-XhoI-BsrGI-NheI
UseCRISPR and LentiviralExpressionMammalianAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-D1R
Plasmid#89160PurposeExpress vaccinia virus D1R in mammalian cellsDepositorInsertVaccinia virus D1R
ExpressionMammalianPromoterCAG promotorAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE (AAV8)
Viral prep#131004-AAV8PurposeReady-to-use AAV8 particles produced from pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE (#131004). In addition to the viral particles, you will also receive purified pAAV-hSyn-GCaMP 6m-p2A-ChRmine-Kv2.1-WPRE plasmid DNA. hSyn-driven expression of calcium sensor GCaMP6m and bicistronic ChRmine (red-shifted channelrhodopsin). These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
BE4
Plasmid#100802PurposeExpresses BE4 in mammalian cellsDepositorInsertBE
ExpressionMammalianPromoterCMVAvailable sinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSECC
Plasmid#60820Purpose3rd generation vector. Expresses a sgRNA of interest, Cas9 and CreDepositorInsertsCas9
Cre
UseCRISPR, Cre/Lox, Lentiviral, and Mouse TargetingTagsFlagExpressionMammalianMutationBsmBI site eliminated by C->A; E308GPromoterEFS and EFS (after Cas9-2A)Available sinceNov. 19, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Rab5DN(S34N)
Plasmid#35139DepositorInsertRab5a (RAB5A Human)
TagsmCherryExpressionMammalianMutationDominant Negative- S34NPromoterCMVAvailable sinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-TAZ-S89A
Plasmid#32840DepositorAvailable sinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLOX-CW-CRE
Plasmid#12238DepositorInsertCRE
UseCre/Lox and LentiviralExpressionMammalianMutationCMV promoter is upstream of nls-CRE. LoxP in 3…Available sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-TO-hNGN2
Plasmid#172115PurposePiggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expressionInsertsTagsT2A-mycNLS-mTagBFP2ExpressionMammalianPromoterEF1a and TRE3GAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (AAV1)
Viral prep#75470-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry (#75470). In addition to the viral particles, you will also receive purified pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry plasmid DNA. CAG-driven, Flp recombinase-dependent expression of channelrhodopsin H134R mutant fused to mCherry. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsmCherryAvailable sinceMarch 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (AAV Retrograde)
Viral prep#100854-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV.Syn.NES-jRGECO1a.WPRE.SV40 (#100854). In addition to the viral particles, you will also receive purified pAAV.Syn.NES-jRGECO1a.WPRE.SV40 plasmid DNA. Synapsin-driven GECO1a calcium sensor. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynAvailable sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1651-sgRNA(F+E)-sgGal4
Plasmid#100549PurposesgGal4-expressing vector modified from Addgene plasmid #51024DepositorInsertsgGal4
UseCRISPRTagssgRNAExpressionMammalianPromoterU6Available sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUAS-NanoLuc
Plasmid#87696PurposeGal4VP16 driven expression of NanoLucDepositorInsertNanoLuc
Tagsnuclear localization signalExpressionMammalianAvailable sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6
Plasmid#64222PurposeExpression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2ADepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6ExpressionMammalianPromoterCBh and U6Available sinceMay 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by