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pET28a(DAP10)

Plasmid

#177904

Purpose

Bacterial expression of his10-tagged human DAP10 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

DAP10 (aa 70-92 only) (HCST Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pBXNPHM3-Nb_MsbA#1

Plasmid

#186428

Purpose

Plasmid for bacterial expression of MsbA binding Nanobody Nb_MsbA#1

Depositor

Markus Seeger

Article

Galazzo et al Sci Adv. 2022 Oct 14;8(41):eabn6845. doi

Insert

Nanobody Nb_MsbA#1

Use

Affinity Reagent/ Antibody

Tags

3C cleavage site, His Tag, Maltose Binding Protei…

Promoter

pBAD

Available Since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pHS1068

Plasmid

#205962

Purpose

NZ_CP025815 DinG HNH proteins (E. coli codon optimized) and CRISPR array in pACYCDuet-1 with Lac promoters

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

DinG HNH proteins and CRISPR array

Expression

Bacterial

Promoter

Lac (proteins), pJ23119 (crRNA)

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf48

Plasmid

#12736

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf48

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
GST-PS-Sos1 (PRM) - Cys (XSB364)

Plasmid

#78506

Purpose

Construct for expressing Sos1 proline-rich motif in E. Coli, with a C-terminal Cys for labeling

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

Sos1 -Cys (PRM) (SOS1 Human)

Tags

GST followed by a prescission protease claevage s…

Expression

Bacterial

Available Since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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CoV2-N-M234I

Plasmid

#177955

Purpose

Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (M234I). Used for generating RNA packaging virus-like particles.

Depositor

Jennifer Doudna

Article

Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

Insert

SARS-CoV-2 Nucleocapsid (M234I) (N )

Expression

Mammalian

Mutation

M234I

Available Since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_06-mEGFP

Plasmid

#194684

Purpose

CMV driven expression of the calcium recorder Caprola_06 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pFUW-TetO-SUB1

Plasmid

#178440

Purpose

doxycycline-inducible expression of Human SUB1 in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

SUB1 (SUB1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse Egr4

Plasmid

#108096

Purpose

Expresses mouse Egr4 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr4 (Egr4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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C1-F-tractin(delta1-6)-mCitrine

Plasmid

#155219

Purpose

F-actin bidning mutant of F-tractin

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine (Itpka Rat)

Tags

mCitrine

Expression

Mammalian

Mutation

Deletion of first 6 AA from F-tractin (9-14 from …

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEJS1026-pMCSG7-HpaCas9

Plasmid

#121540

Purpose

Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

HpaCas9

Expression

Bacterial

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe(puro) mouse Egr4

Plasmid

#108105

Purpose

Retroviral vector for expression of mouse Egr4

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr4 (Egr4 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

SV40

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_04-mEGFP_WRPE-SV40

Plasmid

#194688

Purpose

hSyn1 driven expression of the calcium recorder Caprola_04 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_04-mEGFP

Use

AAV

Tags

mEGFP

Promoter

hSyn1

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pXPA-fapO-RgTALsyn

Plasmid

#100946

Purpose

Expression of Tyrosine Ammonia Lyase from constitutive GAP promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

GAP (Constitutive)

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pEJS1027-pMCSG7-SmuCas9

Plasmid

#121541

Purpose

Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

SmuCas9

Expression

Bacterial

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-Hu_Plk5(WT)

Plasmid

#136319

Purpose

Mammalian expression of human Plk5-WT with N-terminal EGFP

Depositor

Guillermo de Cárcer, Marcos Malumbres

Article

de Carcer et al Mol Cell Biol. 2011 Mar;31(6):1225-39. d

Insert

PLK5 (PLK5 Human)

Expression

Mammalian

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSirenRetroQ(puro)-shEgr3

Plasmid

#108115

Purpose

pSIREN-RetroQ vector containing shRNA sequence to Egr3

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

shRNA to Egr3 (Egr3 Mouse)

Use

RNAi and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pLIX402 caPHF2

Plasmid

#91701

Purpose

inducible lentiviral expression of constitutively active PHF2

Depositor

Bob Weinberg

Article

Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

Insert

PHF2 (PHF2 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

caPHF2; S757D, S899D, S954D, S1056D

Promoter

TRE promoter, Tet ON

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only
pMIG-Flag-RIPK1-D324A

Plasmid

#102678

Purpose

retroviral expression plasmid for RIPK1-D324A

Depositor

Hasam Habelhah

Article

Zhang et al Mol Cell Biol. 2015 Oct;35(19):3324-38.

Insert

RIPK1 (RIPK1 Human)

Use

Retroviral

Tags

Flag and IRES-GFP

Expression

Mammalian

Mutation

CHANGE Glutamate 324 to Alanine

Promoter

unknown

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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Arp2_pLib

Plasmid

#173676

Purpose

Arp2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

Arp2 (ACTR2 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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