We narrowed to 19,051 results for: Tec

1. pETcon-pGAL1-Aga2-α-Synuclein-c_myc

   Plasmid

   #225222

   Purpose

   Yeast optimized human alpha synuclein fused with aga2 protein gene under gal1 promoter for the expression of alpha synuclein in yeast surface display.

   Depositor

   Urartu Seker

   Article

   Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

   Inserts

   α-Synuclein
   Aga2
   

   Tags

   GS linker, HA Tag, and c-myc Tag

   Expression

   Yeast

   Promoter

   GAL1

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pINQ-ON10

   Plasmid

   #198689

   Purpose

   Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)

   Depositor

   Gualberto González Sapienza

   Article

   Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

   Insert

   anti-SARS-CoV-2 Nucleocapsid nanobody ON10
   

   Tags

   6xHis tag, HA tag, OmpA signal peptide, and Ribos…

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pINQ-NbH4

   Plasmid

   #198690

   Purpose

   Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)

   Depositor

   Gualberto González Sapienza

   Article

   Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

   Insert

   anti-SARS-CoV-2 Nucleocapsid nanobody H4
   

   Tags

   6xHis tag, Avitag, OmpA signal peptide, and Ribos…

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-U6-sgLacZ-U6-sgGFP-hSyn1-mCherry

   Plasmid

   #208834

   Purpose

   Control plasmid expresses mCherry under the hSyn1 promoter with sgRNAs against LacZ and GFP

   Depositor

   Ben Emery

   Article

   Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-U6-sgMap3K12(1)-U6-sgMap3K12(2)-hSyn1-mCherry

   Plasmid

   #208835

   Purpose

   Knockout of Map3K12 (DLK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

   Depositor

   Ben Emery

   Article

   Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-U6-sgMap3K13(1)-U6-sgMap3K13(2)-hSyn1-mCherry

   Plasmid

   #208836

   Purpose

   Knockout of Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

   Depositor

   Ben Emery

   Article

   Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-U6-sgMap3K13(1)-U6-sgMap3K12(1)-hSyn1-mCherry

   Plasmid

   #208837

   Purpose

   Knockout of Map3K12 (DLK) and Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

   Depositor

   Ben Emery

   Article

   Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   hSyn1

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRRL-mouse cGAS #2-gRNA-Cas9-Puro

   Plasmid

   #186891

   Purpose

   gRNA targeting mouse cGAS (with Cas9 insert)

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   Cas9 (Cgas Mouse)
   

   Use

   Lentiviral

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRE3G-mouse cGAS-GFP

   Plasmid

   #186877

   Purpose

   Doxycycline-dependent expression of mouse cGAS gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   Mouse cGAS with C-terminal GFP fusion (Cgas Mouse)
   

   Use

   Retroviral

   Tags

   GFP

   Promoter

   TRE3G

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE3G-gibbon cGAS-HA

    Plasmid

    #186862

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Gibbon cGAS with C-terminal HA tag (CGAS Hylobatidae)
    

    Use

    Retroviral

    Tags

    HA

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGB3_alpha2_BeYDV geminino 2.0 (no ATG)-eGFP (GB5178)

    Plasmid

    #225443

    Purpose

    Geminino 2.0-no ATG on CDS. BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1.

    Depositor

    Diego Orzaez

    Article

    Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

    Insert

    BeYDV LIR1 + 2nd half intron ICON MP + eGFP w/o ATG + t35S:SIR + P35s + 1st half of intron from ICON MP + BeYDV LIR1
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDGB3_o1_LIR-2nd intron-3'eGFP-T35S-SIR-p35S-5'eGFP-1st intron-LIR (GB5177)

    Plasmid

    #225442

    Purpose

    BeYDV LIR:2nd intron + 3'eGFP +T35S:BeYDV SIR + p35S + 5'eGFP + 1st intron:BeYDV LIR for INPACT configuration.

    Depositor

    Diego Orzaez

    Article

    Garcia-Perez et al Plant Biotechnol J. 2025 Jan;23(1):141-1

    Insert

    LIR:2nd intron:3'eGFP:T35S:SIR:p35S:5'eGFP:1st intron:LIR
    

    Use

    Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. SS-Myc-HexaPro-Foldon (MTK 3a) (pZYW077)

    Plasmid

    #194233

    Purpose

    Mammalian Toolkit part 3a encoding Jason McClellan lab's 6-proline Spike extracellular domain

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    CD8a Signal Sequence-Myc Tag-SARS-CoV-2 Spike1-1258 (S Synthetic)
    

    Use

    Synthetic Biology

    Promoter

    None

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAPM-miR30-TMEM173-ts1

    Plasmid

    #174283

    Purpose

    TMEM173 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    TMEM173 shRNA (STING1 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAPM-miR30-TMEM173-ts2

    Plasmid

    #174284

    Purpose

    TMEM173 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    TMEM173 shRNA (STING1 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAPM-miR30-TMEM173-ts3

    Plasmid

    #174285

    Purpose

    TMEM173 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    TMEM173 shRNA (STING1 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAPM-miR30-LRPPRC-ts3

    Plasmid

    #174270

    Purpose

    LRPPRC knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    LRPPRC shRNA (LRPPRC Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAPM-miR30-EIF2AK2-ts2

    Plasmid

    #174248

    Purpose

    EIF2AK2 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    EIF2AK2 shRNA (EIF2AK2 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAPM-miR30-EIF2AK2-ts3

    Plasmid

    #174249

    Purpose

    EIF2AK2 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    EIF2AK2 shRNA (EIF2AK2 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAPM-miR30-CAPRIN1-ts1

    Plasmid

    #174229

    Purpose

    CAPRIN1 knockdown

    Depositor

    Jeremy Luban

    Article

    Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

    Insert

    CAPRIN1 shRNA (CAPRIN1 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    SFFV

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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