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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,856 results for: 199

Showing: 941 - 960 of 11856 results

  2. mCit-Snap25b D179K A199R

    Plasmid
    #53259
    Purpose
    SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    SNAP-25B (Snap25 Rat)
    Use
    Tags
    4aa-linker and mCitrine
    Expression
    Mammalian
    Mutation
    D179K and A199R
    Promoter
    CMV
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pCA528 CHMP1B 143-199

    Plasmid
    #108255
    Purpose
    Expresses C-terminal 143-199 residues of CHMP1B with non-native N-term cysteine for fluorescent labeling; Internal ID: WISP18-2
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    CHMP1B residues 143-199 (CHMP1B Human)
    Use
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    Contains nonnative Gly-Cys for fluorescent labeli…
    Promoter
    T7
    Available since
    Nov. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    108255-plasmid-map-sequence-id-245252

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  7. Nav1.5_1-2linker411-717_PPSYtoAASA1960-1996

    Plasmid
    #187732
    Purpose
    Express a chimera of the Nav1.5 linker between the first and second transmembrane domain with the motif surrounding PPSY; residues 1974-1977 PPSY mutated to AASA
    Depositor
    Sandra Gabelli
    Article
    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi
    Insert
    Nav1.5 1,2linker residues 411-717 and Nav1.5 residues 1960-1996 PPSY mutated to AASA (SCN5A Human)
    Use
    Tags
    His-TEV
    Expression
    Bacterial
    Mutation
    deletion of residues 1-410 and 718-1959 and 1997-…
    Promoter
    Available since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    187732-plasmid-map-sequence-id-372456

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  12. pCm N-term SMT3 NikJC199U

    Plasmid
    #174210
    Purpose
    Expression plasmid with a TEV removable C-term 8xHis Tag, an N-term SUMO tag and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Article
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Use
    Tags
    8xHis, SMT3, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174210-plasmid-map-sequence-id-342456

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  13. pCm C-term SMT3 NikJC199U

    Plasmid
    #174362
    Purpose
    Expression plasmid with a TEV removable C-term 8xHis Tagged SUMO tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Article
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Use
    Tags
    8xHis, SMT3, and TEV
    Expression
    Bacterial
    Mutation
    C199U
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174362-plasmid-map-sequence-id-342846

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  14. pCm N-term MBP NikJC199U

    Plasmid
    #174364
    Purpose
    Expression plasmid with a TEV removable N-term MBP tag, a TEV removable C-term 8xHis tag, and Chloramphenicol resistance. NikJ C199U cloned in the cloning site.
    Depositor
    Brandon Greene
    Article
    Caceres et al Methods Enzymol. 2022;662:119-141. doi:
    Insert
    nikJ, nikkomycin biosynthesis protein P1 [Streptomyces tendae]
    Use
    Tags
    8xHis, MBP, and TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174364-plasmid-map-sequence-id-342851

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  17. LSB-hsa-miR-199b-3p

    Plasmid
    #103321
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-199b-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-199b-3p target (MIR199B Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103321-plasmid-map-sequence-id-195691

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  18. LSB-hsa-miR-199b-5p

    Plasmid
    #103322
    Purpose
    Used to sense miRNA activity using fluorescence based tools. hsa-miR-199b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments
    Depositor
    Ron Weiss
    Article
    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:
    Insert
    hsa-miR-199b-5p target (MIR199B Human)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1a
    Available since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    103322-plasmid-map-sequence-id-195693

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  20. pPuro3.1(+)_Strep-Lrrk2(D1994S)-Myc

    Plasmid
    #161584
    Purpose
    expression of Myc-tagged kinase-dead mouse Lrrk2 containing D1994S mutation
    Depositor
    Maik Hintze
    Insert
    Lrrk2 (Lrrk2 Mouse)
    Use
    Tags
    Myc tag and StrepII
    Expression
    Mammalian
    Mutation
    D1994S
    Promoter
    CMV
    Available since
    Dec. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    161584-plasmid-map-sequence-id-318588

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Showing: 941 - 960 of 11856 results