Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 4,974 results for: codon optimized

Clear keywords
Showing: 941 - 960 of 4974 results

  1. pTE3333

    Plasmid
    #107540
    Purpose
    Expresses Fn crRNA and human codon optimized FnCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Publication
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Fn crRNA
    hFnCpf1(RR mutant)
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N607R, K671R
    Promoter
    CMV and human U6
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107540-plasmid-map-sequence-id-207239

    Enlarge

  2. pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro

    Plasmid
    #141383
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-orf3a (ORF3a SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 8, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141383-plasmid-map-sequence-id-288094

    Enlarge

  3. pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)

    Plasmid
    #71720
    Purpose
    Expressing an mRuby-tagged TIR1 in the soma of C. elegans
    Depositor
    Abby Dernburg
    Publication
    Zhang et al Development. 2015 Nov 9. pii: dev.129635
    Insert
    TRANSPORT INHIBITOR RESPONSE 1 (TIR1 Mustard Weed)
    Tags
    mRuby
    Expression
    Worm
    Mutation
    codon-optimized for expression in C. elegans; Two…
    Promoter
    eef-1A.1 (eft-3)
    Available since
    Dec. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71720-plasmid-map-sequence-id-243709

    Enlarge

  4. pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-Puro

    Plasmid
    #141390
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-orf8 (ORF8 SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 8, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141390-plasmid-map-sequence-id-288086

    Enlarge

  7. pLVX-EF1alpha-SARS-CoV-2-nsp12-2xStrep-IRES-Puro

    Plasmid
    #141378
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp12 (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141378-plasmid-map-sequence-id-288083

    Enlarge

  8. pLVX-EF1alpha-SARS-CoV-2-nsp5-2xStrep-IRES-Puro

    Plasmid
    #141370
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp5 (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141370-plasmid-map-sequence-id-288077

    Enlarge

  9. 3xFLAG-dCas9/pMXs-puro

    Plasmid
    #51240
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Publication
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Aug. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51240-plasmid-map-sequence-id-410075

    Enlarge

  10. pAT15547_PEmax-SuperFi-Cas9

    Plasmid
    #184377
    Purpose
    Mammalian expression of SuperFi-Cas9 PEmax prime editor
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PEmax-SuperFi-Cas9
    Use
    CRISPR
    Tags
    SV40 NLS and c-myc NLS
    Expression
    Mammalian
    Mutation
    H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V101…
    Promoter
    CMV
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184377-plasmid-map-sequence-id-362139

    Enlarge

  12. pKMD106h - intein-Cas9(C574)

    Plasmid
    #64191
    Purpose
    Expresses intein-Cas9(C574) in mammalian cells
    Depositor
    David Liu
    Publication
    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
    Use
    CRISPR
    Tags
    NLS - 3x FLAG (C terminal on insert)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    64191-plasmid-map-sequence-id-107550

    Enlarge

  13. pLVX-EF1alpha-SARS-CoV-2-nsp13-2xStrep-IRES-Puro

    Plasmid
    #141379
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp13 (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141379-plasmid-map-sequence-id-288091

    Enlarge

  14. pLVX-EF1alpha-SARS-CoV-2-nsp7-2xStrep-IRES-Puro

    Plasmid
    #141373
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp7 (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 6, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141373-plasmid-map-sequence-id-288040

    Enlarge

  16. AAV pEF1a-DIO-FLPo-WPRE-hGHpA (AAV1)

    Viral prep
    #87306-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from AAV pEF1a-DIO-FLPo-WPRE-hGHpA (#87306). In addition to the viral particles, you will also receive purified AAV pEF1a-DIO-FLPo-WPRE-hGHpA plasmid DNA. EF1a-driven, Cre dependent expression of the site-specific recombinase FlpO. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Li Zhang
    Publication
    Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.
    Promoter
    EF1a
    Available since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only

  17. pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro

    Plasmid
    #141374
    Purpose
    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus
    Depositor
    Nevan Krogan
    Publication
    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:
    Insert
    SARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)
    Use
    Lentiviral
    Tags
    2xStrep
    Expression
    Mammalian
    Mutation
    human codon optimized
    Promoter
    EF1alpha
    Available since
    April 9, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    141374-plasmid-map-sequence-id-288076

    Enlarge

  19. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104910
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104910-plasmid-map-sequence-id-206061

    Enlarge

Showing: 941 - 960 of 4974 results