We narrowed to 6,231 results for: nls

1. pEGFP-C1 RPEL1-EGFP-3XNLS

   Plasmid

   #58469

   Purpose

   Expresses a nuclear-targeted monomeric actin reporter, consisting of the RPEL1 domain from MAL/MRTF, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Mol Biol Cell. 2013 Apr;24(7):982-94. do

   Inserts

   MRTF-A RPEL1 (Mrtfa Mouse, Synthetic)
   3X NLS with 3X stop codons
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Akt-STOPS-NLS

   Plasmid

   #175007

   Purpose

   Nuclear-targeted Akt-STOPS.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   mCherry-Akt-STOPS-NLS
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PET-B-evoSpCas9-NLS-6xHis

   Plasmid

   #207390

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. UbC NLS-HA-MCP-YFP

   Plasmid

   #31230

   Depositor

   Robert Singer

   Article

   Grunwald et al Nature. 2010 Sep 30. 467(7315):604-7.

   Insert

   NLS-HA-MCP-YFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. CSII-prEF1a-E2-Crimson-NLS

   Plasmid

   #125263

   Purpose

   Fluorescent reporter for cell size

   Depositor

   Jan Skotheim

   Article

   Berenson et al Mol Biol Cell. 2019 Nov 15;30(24):2985-2

   Insert

   E2-Crimson-NLS
   

   Use

   Lentiviral

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57-mcGAS-LL/RK-NLS/D307A

   Plasmid

   #185459

   Purpose

   Inducible nuclear cGAS with D307A mutation

   Depositor

   Shitao Li

   Article

   Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

   Insert

   cGAS
   

   Use

   Lentiviral

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   L157R, L159K, D307A

   Promoter

   Available Since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn1-2xNLS-mTurquoise2

   Plasmid

   #118025

   Purpose

   An AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoter

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   2xNLS-mTurquoise2
   

   Use

   AAV

   Tags

   NLS

   Expression

   Mutation

   Promoter

   hSyn1

   Available Since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-TetOn-NLS-mCherry

   Plasmid

   #193762

   Purpose

   Doxycycline-inducible (TetOn) mCherry with SV40 NLS, control construct

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   NLS-mCherry
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mutation

   SV40 NLS fused to mCherry

   Promoter

   TRE

   Available Since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. dCas9-NS3-NLS/VPR

   Plasmid

   #112244

   Purpose

   Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA.

   Depositor

   John Ngo

   Article

   Tague et al Nat Methods. 2018 Jul;15(7):519-522. doi

   Insert

   dCas9-NS3-NLS/VPR
   

   Use

   Tags

   AU1 (N term on NS3) and HA (C term on NS3)

   Expression

   Mammalian

   Mutation

   NS3 mutant T54A

   Promoter

   CMV

   Available Since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-NLS-PhoCl-mCherry

    Plasmid

    #87691

    Purpose

    Express NLS-PhoCl-mCherry in mammalian cells

    Depositor

    Robert Campbell

    Article

    Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

    Insert

    NLS-PhoCl-mCherry
    

    Use

    Tags

    nuclear localization signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDNA-3xFLAG-NLS-TPP1

    Plasmid

    #53585

    Purpose

    Mammalian expression of 3xFLAG tagged, nuclear localized TPP1, N-terminal.

    Depositor

    Steven Artandi

    Article

    Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

    Insert

    TPP1 (ACD Human)
    

    Use

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Silent mutation to eliminate PacI site

    Promoter

    CMV

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

12. A3Ai-Cas9n-UGI-NLS

    Plasmid

    #109425

    Purpose

    Expresses human APOBEC3A containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-Like 3A (APOBEC3A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Insertion of an L1 intron into the coding sequenc…

    Promoter

    CMV

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PET-WT SpCas9-NLS-6xHis

    Plasmid

    #207373

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    WT SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-T7-NlaCas3-NLS-6xHis

    Plasmid

    #180214

    Purpose

    Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Insert

    NlaCas3c
    

    Use

    Tags

    6xHis and NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-eGFP-NLS-RNF168

    Plasmid

    #133977

    Purpose

    mammalian expression vector of eGFP tagged wild type RNF168

    Depositor

    Daniel Durocher

    Article

    Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

    Insert

    RNF168 (RNF168 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

    Promoter

    CMV

    Available Since

    Nov. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pEJS887_pTetR-P2A-BFPnls/sgAlpha

    Plasmid

    #108651

    Purpose

    Alpha satellite repeats targeting Spy sgRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

    Inserts

    Alpha satellite repeats targeting sgRNA
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    No

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and hPGK

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEN019-SpCas9-hiNLS-t-M1M4

    Plasmid

    #234456

    Purpose

    Expresses hiNLS variant t-M1M4 inside 0C triNLS-SpCas9-NGG

    Depositor

    Ross Wilson

    Article

    Noel et al CRISPR J. 2025 Apr;8(2):105-119. doi: 10

    Insert

    SpCas9 hiNLS t-M1M4
    

    Use

    CRISPR

    Tags

    His6, Nucleoplasmin, SV40, and c-Myc

    Expression

    Bacterial

    Mutation

    Cys at positions 80 and 574 have been mutated to …

    Promoter

    Available Since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGTag-NLS-TagRFP-SV40

    Plasmid

    #117805

    Purpose

    Donor for precise CRISPR directed genomic integration using the GeneWeld method

    Depositor

    Jeffrey Essner

    Article

    Wierson et al Elife. 2020 May 15;9. pii: 53968. doi: 1

    Insert

    TagRFP
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-sACt-mCherry-NLS

    Plasmid

    #138214

    Purpose

    Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

    Depositor

    Jin Zhang

    Article

    Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

    Insert

    sACt-mCherry-NLS (Adcy10 Rat)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-FNLSHiFiNG-P2A-Puro

    Plasmid

    #136903

    Purpose

    Lentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFiNG
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Expression

    Mutation

    R691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,T…

    Promoter

    EF1s

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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