We narrowed to 18,824 results for: nls
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Plasmid#87115PurposeSpCas9 expression AAV backbone plasmid under control of nEF promoterDepositorInsertnEF-Cas9
UseAAV and CRISPRTagsHA-NLSExpressionMammalianAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pACUH-GFP11x7-mCherry-α-tubulin
Plasmid#70218PurposeExpresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.DepositorInsertGFP11x7-mCherry-α-tubulin
TagsGFP11x7 and mCherryExpressionInsectAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-LucOS
Plasmid#22777DepositorInsertOVA-SIY
UseCre/Lox and LentiviralTagsluciferaseExpressionMammalianAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
dCas13d-dsRBD-APEX2
Plasmid#154939PurposeTetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTetON-APEX2-V5-BPNLS-dRfxCas13d-dsRBD-BPNLS-P2A-GFP
TagsV5ExpressionMammalianPromoterTRE3GAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX330A-nBRD4/PITCh
Plasmid#91794PurposePITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSpCas9
UseCRISPRExpressionMammalianPromoterchicken β-actin promoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Fuw-dCas9-Dnmt3a_IM
Plasmid#84478PurposeLentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)DepositorInsertdCas9-Dnmt3a_IM
UseLentiviralExpressionMammalianAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSFFV_sfCherry3C(1-10)
Plasmid#117482PurposeLentiviral vector for constitutive expression of sfCherry3C(1-10)DepositorInsertsfCherry3C(1-10)
UseLentiviralExpressionMammalianMutation5 substitutions (K45R, G52D, T106A, K182R, N194D)…PromoterSFFVAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRIP-CMV-tagRFP-FLAG-cGAS
Plasmid#86676PurposeExpresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.DepositorInserttagRFP-FLAG-cGAS
UseLentiviralAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMAL-his-LbCpf1-EC
Plasmid#79008PurposeBacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)DepositorInsertLbCpf1
TagsMBP-6xHis-NLSExpressionBacterialPromotertac promoterAvailable sinceJuly 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmax pona 12xTRICK 24xMS2SL
Plasmid#64542PurposeTRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert12x PP7 stem-loops
Tags24xMS2 stem loops, HA, NLS, and dNP799ExpressionMammalianPromoterponAAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCRISPR v2-sgControl
Plasmid#125836PurposeKO controlDepositorInsertControl
UseLentiviralAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_PGK_LaG16_2_synNotch_Gal4VP64
Plasmid#85421PurposeLentiviral vector for expression of LaG16_2 GFP nanobody Gal4VP64 synthetic NotchDepositorInsertLaG16_2 GFP nanobody synNotch Gal4VP64
UseLentiviralPromoterPGKAvailable sinceAug. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX601-GFP
Plasmid#84040PurposeStaphylococcus aureus (SaCas9) conjugated with GFPDepositorInsertSaCas9
UseAAV and CRISPRTagsNLS and T2A-EGFPExpressionMammalianPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Luc.Cre empty vector
Plasmid#20905DepositorTypeEmpty backboneUseCre/Lox and LentiviralExpressionMammalianAvailable sinceApril 22, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBABE-puro-NLS-LSSmKate2
Plasmid#34586DepositorInsertNLS-LSS-mKate2
UseRetroviralExpressionMammalianMutationK69Y/P131T/S148G/M167D/T183S/M196V compared to mK…Available sinceFeb. 21, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYLCRISPR/Cas9Pubi-H
Plasmid#66187PurposeExpression of Cas9 in plants, maize ubiquitin promoter (Pubi), hygro selectionDepositorInsertCas9
ExpressionPlantMutationplant-codon optimized with higher GC contents (62…Promotermaize ubiquitin promoter (Pubi)Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHsp70-Cas9
Plasmid#45945PurposeThe codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.DepositorPublicationInsertCodon optimized Cas9
UseCRISPRTags3x FLAGMutationSilent change from C to A at bp 834 of insertAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-MMLVgag-3xNES-ABE8e
Plasmid#181751PurposeMMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMMLVgag-3xNES-ABE8e
TagsFlagExpressionMammalianPromoterCMVAvailable sinceFeb. 8, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSFFV_sfCherry2(1-10)
Plasmid#82603PurposeLentiviral vector for constitutive expression of sfCherry2(1-10)DepositorInsertsfCherry2(1-10)
UseLentiviralExpressionMammalianMutationchanged Glu 118 to Gln, changed Thr 128 to IlePromoterSFFVAvailable sinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC152-dual-dCas9VP64-sgExpression
Plasmid#48238PurposeDual expression construct expressing both dCas9VP64 and sgRNA from separate promotersDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCas9
UseCRISPRTagsHA Tag, HA-Tag, and VP64ExpressionMammalianMutationD10A;H840AAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by