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pLJC2-NADK (D184A)-3xFLAG

Plasmid

#233889

Purpose

lentiviral overexpression vector for NADK (D184A)

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

NADK (NADK Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

D184A

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC2-Blast-Rap2A-3xFLAG

Plasmid

#233890

Purpose

lentiviral overexpression vector for RAP2A

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

RAP2A (RAP2A Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC6-NADK (D184A)-3xFLAG

Plasmid

#233894

Purpose

lentiviral overexpression vector NADK (D184A)

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

NADK (NADK Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

D184A

Promoter

Ubc

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-DD-T6B-MUT-YFP

Plasmid

#235146

Purpose

Expresses the inducible mutant T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused mutant T6B peptide

Use

AAV

Mutation

5 tryptophan residues of T6B-WT mutated

Promoter

L7-6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-FLAG-HA-T6B-MUT-YFP

Plasmid

#235142

Purpose

Expresses the mutant T6B peptide fused with YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

mutant T6B peptide

Use

AAV

Tags

FLAG/HA

Mutation

5 tryptophan residues of T6B-WT mutated

Promoter

L7-6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mCAMSAP3-Myc

Plasmid

#236536

Purpose

For expression of CAMSAP3 (mouse) in mammalian cells

Depositor

Sandra Citi

Article

Flinois et al MicroPubl Biol. 2024 Oct 14;2024:10.1791

Insert

CAMSAP3 (Camsap3 Mouse)

Tags

GFP and Myc

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pEt28a-H6-SUMO-SilkCatcher

Plasmid

#230902

Purpose

Bacterial expression of SilkCatcher

Depositor

Sesilja Aranko

Article

Fan et al Angew Chem Int Ed Engl. 2023 Mar 6;62(11

Insert

H6-SUMO-SilkCatcher

Tags

HisTag, thrombin, SUMO

Expression

Bacterial

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pISEre1reRNA

Plasmid

#226821

Purpose

Expressing ISEre1 guide RNA targeting maeB site controled by PJ23119

Depositor

Sheng Yang

Article

Zhou et al Biotechnol J. 2025 Mar;20(3):e70005. doi

Insert

ISEre1 none target guide RNA

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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GB4003

Plasmid

#215248

Purpose

Module for the constitutive expression of PIF6 and PhyB:VP16

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

PIF6-PhyB:VP16

Use

Synthetic Biology

Mutation

V234L in PIF6- please see dep. sequence, BsaI and…

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLEX302_circSMAD2_OE

Plasmid

#215238

Purpose

Overexpression of pLEX302_circSMAD2(2,7)_OE expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

circSMAD2

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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shRNA_LacZ

Plasmid

#215217

Purpose

Supression of shLacZ expression (Negative control)

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

LacZ shRNA

Use

Lentiviral

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pMZ103-rbs1-m1

Plasmid

#204463

Purpose

This is a biosensor with improved specificity to 3OC12-HSL, containing LasR A127N mutation.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

Inserts

quorum sensing regulator LasR (lasR )
eYFP

Use

Synthetic Biology

Mutation

LasR A127N mutation

Promoter

In an operon and PLas

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pMZ103-rbs1-m2

Plasmid

#204464

Purpose

This is a biosensor with improved specificity to 3OC12-HSL, containing LasR L128C mutation.

Depositor

Lauren Andrews

Article

Zeng et al ACS Synth Biol. 2024 Feb 16;13(2):568-58

Inserts

quorum sensing regulator LasR (lasR )
eYFP

Use

Synthetic Biology

Mutation

LasR L128C mutation

Promoter

In an operon and PLas

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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GB4653

Plasmid

#215267

Purpose

TU for the constitutive expression of the flippase (FLP) recombinase with a nopaline synthase (Nos) promoter

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

pNos:FLP:tNos

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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GB4829

Plasmid

#215275

Purpose

TU for the constitutive expression of the N. nambi NPGA gene under the P35S promoter and the TNOS terminator

Depositor

Diego Orzaez

Article

Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

Insert

P35S:NPGA:TNOS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV4.51-mut

Plasmid

#201060

Purpose

Expresses AAV2 Rep and AAV4.51 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV4.51 Cap

Use

AAV

Expression

Mammalian

Mutation

N586R, S587V, N588D, L589R

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV4.52-mut

Plasmid

#201061

Purpose

Expresses AAV2 Rep and AAV4.52 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV4.52 Cap

Use

AAV

Expression

Mammalian

Mutation

N586A, S587G, N588F, L589R

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV4.68-mut

Plasmid

#201064

Purpose

Expresses AAV2 Rep and AAV4.68 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV4.68 Cap

Use

AAV

Expression

Mammalian

Mutation

T591V, D593R, R594G, T596R

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV4.69-mut

Plasmid

#201065

Purpose

Expresses AAV2 Rep and AAV4.69 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV4.69 Cap

Use

AAV

Expression

Mammalian

Mutation

T591P, D593M, R594G, T596G

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pBsVchDonor

Plasmid

#203814

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

Insert

Mini-Tn, aprE cassette

Use

CRISPR

Expression

Bacterial

Available Since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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