We narrowed to 8,785 results for: sgrna

1. pLV U6-sgRNA UbC-tdTomato

   Plasmid

   #106949

   Purpose

   sgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato marker

   Depositor

   Albert Edge

   Article

   Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

   Insert

   sgRNA BsmBI/Esp3I cloning site
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiCrisprV2 sgRNA hPER2c-term #1

   Plasmid

   #179454

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCrisprV2 sgRNA hPER2c-term #2

   Plasmid

   #179455

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCrisprV2 sgRNA hPER2c-term #3

   Plasmid

   #179456

   Purpose

   Lentiviral Crispr/Cas9 plasmid targeting hPER2 at C-terminus to generate C-terminal knock-in

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   sgRNA targeting human PER2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-PpU6P-sgRNA-L5L2

   Plasmid

   #113738

   Purpose

   Gateway entry vector containing attL5 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   P. patens U6 promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-PpU6P-sgRNA-R4R3

   Plasmid

   #113739

   Purpose

   Gateway entry vector containing attR4 and attR3 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   P. patens U6 promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR-PpU6P-sgRNA-L5L4

   Plasmid

   #113741

   Purpose

   Gateway entry vector containing attL5 and attL4 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   P. patens U6 promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-PpU6P-sgRNA-L1R5

   Plasmid

   #113737

   Purpose

   Gateway entry vector containing attL1 and attR5 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   P. patens U6 promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR-PpU6P-sgRNA-L1L2

   Plasmid

   #113735

   Purpose

   Gateway entry vector containing attL1 and attL2 sites, U6 promoter, BsaI sites for protospacer ligation, and sgRNA.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   PpU6P::sgRNA
   

   Use

   CRISPR; Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   P. patens U6 promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCRISPRv2-TRAF6-targeting sgRNA 2

    Plasmid

    #131346

    Purpose

    gRNA targeting mouse TRAF6

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    gRNA targeting mouse TRAF6 (Traf6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

    Plasmid

    #129532

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIA4
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Expression

    Mutation

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZ147-BvCas12b-sgRNA-scaffold

    Plasmid

    #122447

    Purpose

    Expresses sgRNA for BvCas12b in mammalian cells

    Depositor

    Feng Zhang

    Article

    Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

    Insert

    U6-BvCas12b-sgRNA-scaffold, CMV-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLH-sgRNA1-PP7-boxB

    Plasmid

    #75393

    Purpose

    sgRNA1-PP7-boxB

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XPP7-boxB
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. Expression scaffold for dCas9+sgRNA

    Plasmid

    #171799

    Purpose

    PICASSO: Expression scaffold for second subcloning step to properly co-express paired dCas9-fusion+sgRNA

    Depositor

    Stephen Elledge

    Article

    Barber et al Mol Cell. 2021 Aug 4. pii: S1097-2765(21

    Insert

    PICASSO: expression scaffold for proper dCas9-fusion + sgRNA expression
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLH-sgRNA1-MS2-PP7

    Plasmid

    #75392

    Purpose

    sgRNA1-MS2-PP7

    Depositor

    Thoru Pederson

    Article

    Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

    Insert

    sgRNA1-2XMS2-PP7
    

    Use

    CRISPR and Lentiviral

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV U6-sgRNA UbC-GFP

    Plasmid

    #106948

    Purpose

    sgRNA cloning cassette using BsmBI/Esp3I enzymes with GFP marker

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    sgRNA BsmBI/Esp3I cloning site
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pX459-sgRNA2-RPB1-N-term

    Plasmid

    #195111

    Purpose

    Vector for transient expression of spCas9-nuclease (V2.0) and a sgRNA targeting the RPB1 N-term. Puromycin selection (2A-fusion).

    Depositor

    Job Dekker

    Article

    Valton et al Nat Struct Mol Biol. 2022 Dec 8. doi: 10

    Insert

    sgRNA against first exon of RPB1 (N-terminal)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. sgRNA1_Tet-inducible Luciferase reporter

    Plasmid

    #64161

    Purpose

    Photoactivatable transcription system. Mammalian expression of sgRNA1 to target Tet-inducible-luciferase reporter.

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1 for Tet-inducible Luciferase reporter
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available Since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

    Plasmid

    #196195

    Purpose

    Editing human TSC2 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    TSC2 (TSC2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. SpyCas9 EMX1-nicking sgRNA

    Plasmid

    #169859

    Purpose

    SpyCas9 nicking sgRNA for EMX1

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SpyCas9 EMX1-nicking sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human U6 promoter

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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