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We narrowed to 2,577 results for: CAG promoter

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Showing: 201 - 250 of 2577 results

  2. pCAG_EGFP-GFE3

    Plasmid
    #79869
    Purpose
    Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.
    Depositor
    Don Arnold
    Article
    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm
    Insert
    GFP-GFE3
    Use
    Synthetic Biology
    Tags
    GFP and HA
    Expression
    Mammalian
    Promoter
    Beta-actin
    Available Since
    July 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    79869-plasmid-map-sequence-id-153506

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  3. pCAG_EGFP-RandE3

    Plasmid
    #79870
    Purpose
    Expresses under the CAG promoter RandE3, a synthetic E3 ligase with random residues in its binding domain; control construct for GFE3.
    Depositor
    Don Arnold
    Article
    Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm
    Insert
    GFP-RandE3
    Use
    Synthetic Biology
    Tags
    GFP and HA
    Expression
    Mammalian
    Promoter
    Beta-actin
    Available Since
    Aug. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    79870-plasmid-map-sequence-id-149706

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  5. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid
    #121170
    Purpose
    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    ABEmax-P2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    P2A-EGFP-ires-puro
    Expression
    Mammalian
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121170-plasmid-map-sequence-id-234985

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  6. CAG-FNLS-T2A-EGFP-ires-puro

    Plasmid
    #121169
    Purpose
    CAG promoter driven expression of FNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    FNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121169-plasmid-map-sequence-id-235002

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  7. CAG-xFNLS-T2A-EGFP-ires-puro

    Plasmid
    #121171
    Purpose
    CAG promoter driven expression of xFNLS BE3 base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    xFNLS-T2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    T2A-EGFP-ires-puro
    Expression
    Mammalian
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121171-plasmid-map-sequence-id-235381

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  10. pCAG-memRFP-3xPax7gRNA

    Plasmid
    #224569
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and membrane RFP reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    mRFP1
    Use
    CRISPR
    Tags
    Membrane localization signal
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224569-plasmid-map-sequence-id-451497

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  17. pCAG-nucGFP11-3xPax7gRNA

    Plasmid
    #224570
    Purpose
    Ubiquitous expression plasmid, CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three unique Pax7 gRNAs with ribozyme self-cleavage sites, and nuclear split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    GFP11-H2B
    Use
    CRISPR
    Tags
    Histone H2B (nuclear localization)
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224570-plasmid-map-sequence-id-451492

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  27. ppyCAG_RNaseH1_WKKD

    Plasmid
    #111905
    Purpose
    Express WKKD mutated RNASEH1 in mammalian cell. The combination of three specific mutations (W43A, K59A, K60A) in the binding domain prevents the enzyme from binding to RNA/DNA hybrids
    Depositor
    Xiang-Dong Fu
    Article
    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.
    Insert
    RNASEH1 (RNASEH1 Human)
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Changed D 210 to N, W 43 to A, K 59 to A, K 60 to…
    Available Since
    July 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    111905-plasmid-map-sequence-id-212872

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  41. pCAG-hCas9D10A

    Plasmid
    #52101
    Purpose
    The expression vector for humanized nickase Cas9 (Cas9D10A) under CAG promoter
    Depositor
    Izuho Hatada
    Insert
    hCas9D10A
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    CAG
    Available Since
    April 30, 2014
    Availability
    Academic Institutions and Nonprofits only

  42. pCAG-MCS-EGFP

    Plasmid
    #164092
    Purpose
    expression of EGFP-tagged proteins under CAG promoter
    Depositor
    Maik Hintze
    Insert
    EGFP
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Jan. 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    164092-plasmid-map-sequence-id-322571

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  43. pCAG-MCS-mRuby2

    Plasmid
    #164093
    Purpose
    expression of mRuby2-tagged proteins under CAG promoter
    Depositor
    Maik Hintze
    Insert
    mRuby2
    Tags
    mRuby2
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164093-plasmid-map-sequence-id-322866

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  44. pCAG-MCS-DsRed

    Plasmid
    #164091
    Purpose
    expression of DsRed-tagged proteins under CAG promoter
    Depositor
    Maik Hintze
    Insert
    DsRed
    Tags
    DsRed
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    164091-plasmid-map-sequence-id-322864

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  46. pCAG-3xGFP11-mem-3xControlgRNA

    Plasmid
    #224583
    Purpose
    Ubiquitous expression plasmid with CAG promoter (CMV immediate early enhancer, chicken beta actin promoter), three Control gRNAs with ribozyme self-cleavage, three membrane split GFP(11) reporter.
    Depositor
    Erica Hutchins
    Article
    Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026
    Insert
    3xGFP11
    Use
    CRISPR
    Tags
    Membrane Localization Signal
    Mutation
    Codon optimized
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    224583-plasmid-map-sequence-id-451495

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Showing: 201 - 250 of 2577 results