We narrowed to 255 results for: petD

1. pET-6xHis-GFP(+6)-L3

   Plasmid

   #205041

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(+6)-L3
   

   Use

   Tags

   GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-6xHis-GFP(+6)-H1

   Plasmid

   #205042

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(+6)-H1
   

   Use

   Tags

   KRRRKK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-6xHis-GFP(+6)-H2

   Plasmid

   #205043

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(+6)-H2
   

   Use

   Tags

   KRRRKKKRRRKK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-6xHis-GFP(+6)-H3

   Plasmid

   #205044

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(+6)-H3
   

   Use

   Tags

   KRRRKKKRRRKKKRRRKK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   E16R, D86K, D112K, D127R, I138R, D207R, N222K, L2…

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-6xHis-GFP(-6)-H2_mCherry(-6)

   Plasmid

   #205049

   Purpose

   Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-H2_mCherry(-6)
   

   Use

   Tags

   MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET-6xHis-GFP(-6)-H2_mCherry(-6)-L1

   Plasmid

   #205050

   Purpose

   Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-H2_mCherry(-6)-L1
   

   Use

   Tags

   GRRGKKSRK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET-6xHis-GFP(-6)-H2_mCherry(-6)-L2

   Plasmid

   #205051

   Purpose

   Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-H2_mCherry(-6)-L2
   

   Use

   Tags

   GRRGKKSRKGRRGKKSRK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET-6xHis-GFP(-6)-H2_mCherry(-6)-L3

   Plasmid

   #205052

   Purpose

   Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-H2_mCherry(-6)-L3
   

   Use

   Tags

   GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-6xHis-GFP(0)-L1

   Plasmid

   #205033

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(0)-L1
   

   Use

   Tags

   GRRGKKSRK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   E16R, I138R, D207R, N222K, L246R

   Promoter

   Available since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-6xHis-GFP(0)-L2

    Plasmid

    #205034

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(0)-L2
    

    Use

    Tags

    GRRGKKSRKGRRGKKSRK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, I138R, D207R, N222K, L246R

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET-6xHis-GFP(0)-L3

    Plasmid

    #205035

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(0)-L3
    

    Use

    Tags

    GRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, I138R, D207R, N222K, L246R

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-6xHis-GFP(0)-H1

    Plasmid

    #205036

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(0)-H1
    

    Use

    Tags

    KRRRKK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, I138R, D207R, N222K, L246R

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET-6xHis-GFP(0)-H2

    Plasmid

    #205037

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(0)-H2
    

    Use

    Tags

    KRRRKKKRRRKK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, I138R, D207R, N222K, L246R

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-6xHis-GFP(0)-H3

    Plasmid

    #205038

    Purpose

    Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

    Insert

    GFP(0)-H3
    

    Use

    Tags

    KRRRKKKRRRKKKRRRKK and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    E16R, I138R, D207R, N222K, L246R

    Promoter

    Available since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pVY-12 CAT-tag3

    Plasmid

    #194627

    Purpose

    antibiotic resistance enzyme with cationic peptide tag to promote biomolecular condensate formation in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al Nat Commun. 2022 Dec 22;13(1):7882. doi:

    Insert

    CAT-(GGSKKRKKR)3
    

    Use

    Tags

    GGSKKRKKRGGSKKRKKRGGSKKRKKR and MGHHHHHGGA

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLacI265-Wasabi

    Plasmid

    #86103

    Purpose

    mWasabi reporter gene gated by temperature-sensitive LacI G265D

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    LacIts
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G265D

    Promoter

    T7A1O4/O3 and pLacI

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTetR89-Wasabi

    Plasmid

    #86104

    Purpose

    mWasabi reporter gene gated by temperature-sensitive TetR A89D

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TetRts
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A89D

    Promoter

    ptetA and ptetR

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTcI38-Wasabi

    Plasmid

    #86117

    Purpose

    mWasabi reporter gene gated by temperature-sensitive Lambda repressor TcI38

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TcI38
    mWasabi
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G

    Promoter

    pR/pL and pR/pL, LacI

    Available since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTcI39-switch

    Plasmid

    #195557

    Purpose

    Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mRFP1
    mWasabi
    TcI39
    

    Use

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-6xHis-GFP(+30)

    Plasmid

    #199164

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+30)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, V21K, D29K, E42K, E44K, D86K, E100K, D112K,…

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCali2

    Plasmid

    #86119

    Purpose

    Multiplex temperature-gated expression of mWasabi and mCherry reporters

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA36
    mWasabi
    TcI
    mCherry
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T and D341A, P60L, D135V, K187R, K202I, L208Q

    Promoter

    pR/pL, pR/pL, pLacI, pTlpA, and pTlpA, pLacI

    Available since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pThermeleon

    Plasmid

    #86120

    Purpose

    Bandpass temperature-gated expression of mWasabi and mCherry reporters

    Depositor

    Mikhail Shapiro

    Article

    Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

    Inserts

    TlpA
    mWasabi
    TcI38
    mCherry
    cI
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G and D3…

    Promoter

    pR/pL, pR/pL, pLacI, and pTlpA

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pKL002

    Plasmid

    #98921

    Purpose

    pET DUET backbone with PROPS voltage indicator and GCaMP6f calcium sensor in the two MCS.

    Depositor

    Joel Kralj

    Article

    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

    Inserts

    PROPS
    GCaMP6f
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Sender plasmid

    Plasmid

    #127677

    Purpose

    Encodes the synthase LuxI for the quorum-sensing molecule C6-HSL.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

    Insert

    LuxI
    

    Use

    Synthetic Biology

    Tags

    LVA

    Expression

    Bacterial

    Mutation

    Promoter

    pT7

    Available since

    Sept. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET-6xHis-GFP(+24)

    Plasmid

    #199163

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+24)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, V21K, D29K, E42K, E44K, D86K, D112K, T115K,…

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDC146

    Plasmid

    #169366

    Purpose

    Expresses Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96

    Depositor

    Axel Brunger

    Article

    Cipriano et al J Biol Chem. 2013 Aug 9;288(32):23436-45

    Insert

    Syntaxin1-265 C145S, S249C, K253C ; SNAP25 ; 6xHis-TEV-Synaptobrevin1-96
    

    Use

    Tags

    6 his tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAM72

    Plasmid

    #227622

    Purpose

    pETDuet-1 Rpn8(1-179)-precission-Strep, H6-precission-Rpn11(2-239, G77P)

    Depositor

    Andreas Martin

    Article

    Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    His-Prescission

    Expression

    Bacterial

    Mutation

    1-179 and aa 2-239, G77P

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTcI39-PRM-wasabi

    Plasmid

    #195555

    Purpose

    Temperature-dependent expression of mWasabi reporter with transcription activation by TcI39

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mWasabi
    TcI39
    

    Use

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET-6xHis-GFP(+36)

    Plasmid

    #199165

    Purpose

    used to express a supercharged GFP variant in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+36)
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. His-drebrin

    Plasmid

    #40362

    Depositor

    Phillip Gordon-Weeks

    Article

    Geraldo et al Nat Cell Biol. 2008 Oct;10(10):1181-9. E

    Insert

    drebrin E2 (DBN1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pET-DUET-GST-eIF4G1-His6, eIF4E

    Plasmid

    #37232

    Depositor

    Jon Lorsch

    Article

    Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

    Inserts

    TIF4631 (TIF4631 Budding Yeast)
    CDC33 (CDC33 Budding Yeast)
    

    Use

    Tags

    GST and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

32. p045

    Plasmid

    #70057

    Purpose

    Co-expresses Syt1-SNARE37aa-linker complex with pDC184

    Depositor

    Axel Brunger

    Article

    Zhou et al Nature. 2015 Sep 3;525(7567):62-7. doi:

    Inserts

    C2AB-linker-SNAP-25_C
    SNAP-25_N
    

    Use

    Tags

    No tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pAM59

    Plasmid

    #227655

    Purpose

    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Δ78–81)

    Depositor

    Andreas Martin

    Article

    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    H6-PreScission and PreScission-Strep

    Expression

    Bacterial

    Mutation

    1-179 and amino acids 2-239, Δ78–81

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAM57

    Plasmid

    #227654

    Purpose

    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)

    Depositor

    Andreas Martin

    Article

    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    H6-PreScission and PreScission-Strep

    Expression

    Bacterial

    Mutation

    1-179 and amino acids 2-239, V80A

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pAM54

    Plasmid

    #227653

    Purpose

    pETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, L132A)

    Depositor

    Andreas Martin

    Article

    Worden et al Nat Struct Mol Biol. 2014 Mar;21(3):220-

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    Rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    H6-PreScission and PreScission-Strep

    Expression

    Bacterial

    Mutation

    1-179 and amino acids 2-239, L132A

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAM71

    Plasmid

    #227621

    Purpose

    pETDuet-1 H6-precission-Rpn11(24-160-GGSGSG-192-221) , Rpn8(1-179)

    Depositor

    Andreas Martin

    Article

    Worden et al Mol Cell. 2017 Sep 7;67(5):799-811.e8. d

    Inserts

    Rpn11 (RPN11 Budding Yeast)
    rpn8 (RPN8 Budding Yeast)
    

    Use

    Tags

    His-Prescission

    Expression

    Bacterial

    Mutation

    1-179 and amino acids 24-160-GGSGSG-192-221

    Promoter

    T7

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTcI38-PRM-wasabi

    Plasmid

    #195554

    Purpose

    Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mWasabi
    TcI38
    

    Use

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SNAP-MIDAS

    Plasmid

    #171604

    Purpose

    Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.

    Depositor

    Tarun Kapoor

    Article

    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

    Insert

    MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
    

    Use

    Tags

    His6 tag and SNAP tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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39. pDC183

    Plasmid

    #106415

    Purpose

    Co-expresses trans SNARE complex with pQZ210 in E. coli

    Depositor

    Axel Brunger

    Article

    Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

    Inserts

    SNAP25 fragment 141-204 (Snap25 Rat, Synthetic)
    SNAP25 Fragment 7-83 (Snap25 Rat, Synthetic)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Fragment 141-204, Codon optimization and Fragment…

    Promoter

    T7

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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40. pET Duet1 His6 SUMO D14

    Plasmid

    #177854

    Purpose

    Bacterial Expression of D14

    Depositor

    Karsten Melcher

    Article

    Zhao et al Cell Res. 2013 Mar;23(3):436-9. doi: 10.

    Insert

    D14
    

    Use

    Tags

    6xHis-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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41. pMtb MCM

    Plasmid

    #138296

    Purpose

    Expression of Mtb MCM small and large subunit

    Depositor

    Ruma Banerjee

    Article

    Ruetz et al Science. 2019 Nov 1;366(6465):589-593. d

    Inserts

    mutA
    mutB
    

    Use

    Tags

    His tag and TEV cleavage site

    Expression

    Bacterial

    Mutation

    inserted TEV cleavage site between His-tag and m…

    Promoter

    T7

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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42. pGV3340

    Plasmid

    #170278

    Purpose

    Expression of mutant HIV-2 RT p51 reverse transcriptase

    Depositor

    Gregory Van Duyne

    Article

    Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

    Insert

    HIV-2 RT p51 (mutant)
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lac

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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43. pET-6xHis-GFP(+36)-DL4

    Plasmid

    #199168

    Purpose

    used to express a supercharged GFP variant that forms inclusion bodies in E. coli

    Depositor

    Allie Obermeyer

    Article

    Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

    Insert

    GFP(+36)-DL4
    

    Use

    Tags

    MGHHHHHHGGA

    Expression

    Mutation

    E16R, T19R, V21K, D29K, E42K, E44K, N49E, D86K, Q…

    Promoter

    T7

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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44. deltaLoop-MIDAS

    Plasmid

    #171605

    Purpose

    Expresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) without the loop region (a.a. 4458-4496) in bacteria.

    Depositor

    Tarun Kapoor

    Article

    Mickolajczyk et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

    Insert

    MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
    

    Use

    Tags

    His6 tag and SNAP tag

    Expression

    Bacterial

    Mutation

    Replaced amino acids 4458-4496 with ASGSGS linker

    Promoter

    Available since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. p36-p37-p38-p40-pET-Duet-1

    Plasmid

    #175049

    Purpose

    Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

    Depositor

    Brian Kelch

    Article

    Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. 6xHis-TEV-ATG3 (Human autophagy E2-like enzyme)

    Plasmid

    #169079

    Purpose

    Human ATG3

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Insert

    ATG3 (ATG3 Human)
    

    Use

    Tags

    6X Histidine Tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac promoter

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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47. MZB035

    Plasmid

    #217834

    Purpose

    Bacterial expression of human AP3D1 (1-617) and AP3S1 AP-3 core hemicomplex

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Inserts

    AP3D1 (AP3D1 Human)
    AP3S1 (AP3S1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Removed residues 618-1153 from AP3D1

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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48. pET-Duet-6xHis-TEV-Nsp8(76-198)

    Plasmid

    #201024

    Purpose

    Bacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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49. p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D

    Plasmid

    #175051

    Purpose

    Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

    Depositor

    Brian Kelch

    Article

    Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    Replication factor C subunit 5 (RFC2, RFC3, RFC4, RFC5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Thr76 to Asp, phosphomimetic mutation

    Promoter

    T7 promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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50. p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA

    Plasmid

    #175050

    Purpose

    Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

    Depositor

    Brian Kelch

    Article

    Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    Replication factor C subunits 2-5, Rfc3-3KtoA-mutation (RFC4, RFC2, RFC3, RFC5 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge