We narrowed to 8,854 results for: sgrna

1. pU6b:sgRNA#3

   Plasmid

   #64247

   Purpose

   expresses sgRNA under U6b promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6c:sgRNA#4

   Plasmid

   #64248

   Purpose

   expresses sgRNA under U6c promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pU6a:sgRNA#2

   Plasmid

   #64246

   Purpose

   expresses sgRNA under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6d:sgRNA#5

   Plasmid

   #64249

   Purpose

   expresses sgRNA under U6d promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. px330_Rosa_sgRNA

   Plasmid

   #97007

   Purpose

   Expresses Cas9 and Rosa26 locus specific sgRNA

   Depositor

   Russell Ray

   Article

   Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

   Insert

   Rosa26 sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHelper_ShCAST_sgRNA

   Plasmid

   #127921

   Purpose

   Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKDsgRNA-NT

   Plasmid

   #89960

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   empty gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available Since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgRNA_expression_vector

   Plasmid

   #210212

   Purpose

   An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

   Depositor

   Albert Jeltsch

   Article

   Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. LCV2_LacZ_sgRNA_2

   Plasmid

   #155093

   Purpose

   lentiviral plasmid expressing Cas9 and gRNA targeting LacZ

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   LacZ_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. psgRNA

    Plasmid

    #114005

    Purpose

    express sgRNA. ColE1, Kan

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    BBa_J23119

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. esgRNA_NbPDS

    Plasmid

    #231147

    Purpose

    T-DNA encoding TRV2 with mobile gRNA targeting NbPDS

    Depositor

    Daniel Voytas

    Article

    Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

    Insert

    mobile gRNA targeting NbPDS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PEBV sub-genomic

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. EC2_1_dCas9_sgRNA

    Plasmid

    #163710

    Purpose

    Yeast low copy plasmid with dCas9 and sgRNA expression cassette

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    dCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Point mutations D10A and H840A

    Promoter

    ScTEF1

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX459_MCM2_sgRNA

    Plasmid

    #232730

    Purpose

    Generates MCM2 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    MCM2 (MCM2 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Promoter

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX459_CDC45_sgRNA

    Plasmid

    #232732

    Purpose

    Generates CDC45 dTAG C ternimal knock-in cell lines

    Depositor

    Junjie Chen

    Insert

    CDC45 (CDC45 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    NO

    Promoter

    Available Since

    March 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. LCV2_AAVS1_sgRNA_2

    Plasmid

    #155088

    Purpose

    lentiviral plasmid expressing Cas9 and gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. psgRNAc

    Plasmid

    #114006

    Purpose

    express sgRNA, p15A, CRM

    Depositor

    David Bikard

    Article

    Cui et al Nat Commun. 2018 May 15;9(1):1912. doi:

    Insert

    gRNA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    BBa_J23119

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pColE1_sgRNA_pos6_AmpR

    Plasmid

    #119149

    Purpose

    Encodes sgRNA targeting position 6 in pColE1_70a_deGFP_KanR

    Depositor

    Vincent Noireaux

    Article

    Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

    Insert

    sgRNA targeting position 6 in pColE1_70a_deGFP_KanR
    

    Use

    Crispr

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pnsgRNA

    Plasmid

    #172717

    Purpose

    Delivery of guide RNA for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    J23119-gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCgsgRNA_crtYf

    Plasmid

    #153338

    Purpose

    Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

    Insert

    crRNA targeting crtYf
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGH224_sgRNA_2xMS2_Puro

    Plasmid

    #85413

    Purpose

    hU6 driven sgRNA vector with 2xMS2 vectors with puro selectable marker

    Depositor

    Michael Bassik

    Article

    Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

    Insert

    puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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