We narrowed to 34,838 results for: CaS;

1. pCasperAUG-Gal4-X

   Plasmid

   #8378

   Depositor

   Leslie Vosshall

   Article

   Vosshall et al Cell 2000 Jul 21;102(2):147-59.

   Insert

   Yeast Gal4 (GAL4 Budding Yeast, fused to first ~30 amino acids of Drosophila ADH)
   

   Use

   Drosophila p-element vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pJCC_083 Cas12i1

   Plasmid

   #171670

   Purpose

   For bacterial expression of Cas12i1 with an N-terminal His-MBP tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   Cas12i1
   

   Use

   Tags

   10X His, TEV protease cleavage site, and maltose-…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ133-RZ-Cas12j2

   Plasmid

   #189784

   Purpose

   Golden Gate entry vector to clone the 3rd Cas12j2(CasΦ) crRNA flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Liu et al Plant Commun. 2022 Sep 20:100453. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCLV3Cas-LT1

   Plasmid

   #210549

   Purpose

   Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporter

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM
   

   Use

   Tags

   Expression

   Plant

   Mutation

   2bp insertion after AAVS1 target sequence that di…

   Promoter

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJCC_039 FnCas12a

   Plasmid

   #171666

   Purpose

   For bacterial expression of FnCas12a with a C-terminal sorting motif and His tag

   Depositor

   Jennifer Doudna

   Article

   Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

   Insert

   FnCas12a
   

   Use

   Tags

   6X His and sortase A sorting motif

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. SaCas9 TadDE

   Plasmid

   #193846

   Purpose

   Express TadDE (with SaCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadDE with SaCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJIT163-2NLSCas9

   Plasmid

   #53064

   Purpose

   Expression of rice codon-optimized Cas9 in plant cells

   Depositor

   Caixia Gao

   Article

   Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

   Insert

   Cas9
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   Rice codon optimized

   Promoter

   2x35S

   Available since

   Aug. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pH-dCas9-PBE

   Plasmid

   #98162

   Purpose

   Expresses CRISPR base editor in rice

   Depositor

   Caixia Gao

   Article

   Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

   Insert

   NLS-APOBEC1-XTEN-dCas9-UGI-NLS
   

   Use

   Rice

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Ubi

   Available since

   July 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. evoFERNY-Cas9NG

   Plasmid

   #203329

   Purpose

   Plasmid for bacterial purification of codon optimized evoFERNY-Cas9NG

   Depositor

   Vijay Sankaran

   Article

   Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

   Insert

   evoFERNY-Cas9NG
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNOC_hfnCas12a-Nlux

    Plasmid

    #176239

    Purpose

    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux under the control of Ribi promoter

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Ribosomal subunit

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO5d.EFS.SpCas9.P2A.PAC

    Plasmid

    #58329

    Purpose

    Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    SpCas9
    EFS (EEF1A1 Human)
    P2A-PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUB-Cas9

    Plasmid

    #86556

    Purpose

    For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Cas9 is codon optimized for C. reinhardtii

    Promoter

    UBIQUITIN10

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. Construct 12 - hUBCp_Cas9_3xNLS_p2a_puroR

    Plasmid

    #81251

    Purpose

    Lentiviral construct for building stable cell lines expressing Cas9 via puromycin selection

    Depositor

    Timothy Lu

    Article

    Perli et al Science. 2016 Aug 18. pii: aag0511.

    Insert

    Cas9-3xNLS
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. p1195-pssAAV.U1a.hNme2Cas9

    Plasmid

    #129534

    Purpose

    AAV vector expressing Nme2Cas9

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    human codon-optimized Nme2Cas9
    

    Use

    AAV

    Tags

    2xNLS and NLS-3xHA-NLS

    Expression

    Mutation

    Promoter

    U1a

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pB-dCas9-PBE

    Plasmid

    #98159

    Purpose

    Expresses CRISPR base editor in maize

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-dCas9-UGI-NLS
    

    Use

    Maize

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA

    Plasmid

    #110624

    Purpose

    A single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNA

    Depositor

    Yannick Doyon

    Article

    Agudelo et al Genome Res. 2020 Jan;30(1):107-117. doi:

    Inserts

    St1Cas9 LMD-9
    sgRNA for St1Cas9
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    LP1B and hU6

    Available since

    May 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas9-beta

    Plasmid

    #169240

    Purpose

    Cas9 and beta expression plasmid for performing gene knockouts via oligo recombineering

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Inserts

    cas9
    beta
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. BB1_23_dCAS9(3xFLAG_SV40NLS)

    Plasmid

    #161478

    Purpose

    BB1 encoding a FLAG tagged dCAS9 with a SV40 NLS

    Depositor

    Brigitte Gasser, Diethard Mattanovich

    Article

    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

    Insert

    dCAS9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    FLAG Tag, SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX165-eSpCas9

    Plasmid

    #140564

    Purpose

    Expresses eSpCas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    K848A K1003A R1060A

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ-dpcoCas9-TV

    Plasmid

    #158409

    Purpose

    CRISPR-dpcoCas9 fused with TV activator for transcriptional activation

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Insert

    dpcoCas9-TV(6xTAL-2xVP64)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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