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NAX_CAGGS_CAS9_m4_KRAB_mcherry

Plasmid

#167878

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
pUC57-HBB-nLuc

Plasmid

#175432

Purpose

Used to generate IVT RNA for translation extracts

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

HBB-nLuc

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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AAV8-cPE-C

Plasmid

#183539

Purpose

Deliver a split compact Prime Editor in vivo

Depositor

Wen Xue

Article

Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

Insert

C-terminal fragment of SpCas9 nickase (H840A), Reverse Transcriptase

Use

AAV

Expression

Mammalian

Promoter

U1A

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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His_Naa80d in pTYB12

Plasmid

#188455

Purpose

Express in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

Naa80 (NAA80 Human)

Tags

intein-CBD, 6xHis

Expression

Bacterial

Mutation

WT

Promoter

T7

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-puro-NKX2-8

Plasmid

#75080

Purpose

retroviral vector holding human NKX2-8 cDNA

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

NKX2-8 (NKX2-8 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

wild-type, full-length, without native 3' UTR

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Beclin1 1-242

Plasmid

#24391

Depositor

Qing Zhong

Article

Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

Insert

Beclin1 (BECN1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Contains amino acids 1-242.

Available Since

April 1, 2010

Availability

Academic Institutions and Nonprofits only
Diffusive 54kDa

Plasmid

#201337

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

EGFP-4PrA

Expression

Mammalian

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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Diffusive 47kDa

Plasmid

#201336

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

EGFP-3PrA

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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PARP2-c098

Plasmid

#216873

Purpose

Bacterial expression vector for E. coli producing PARP2 catalytic protein (without Regulatory domain); with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

Insert

PARP2 catalytic domain (no Regulatory Domain) (PARP2 Human)

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 gp78fl

Plasmid

#37375

Depositor

Allan Weissman

Article

Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

Insert

gp78 (AMFR Human)

Tags

EYFP

Expression

Mammalian

Mutation

P179L and F262L

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP10D(E215Q)-HA

Plasmid

#209248

Purpose

Mammalian expression of ATP10D mutant E215Q

Depositor

Hye-Won Shin

Article

Naito et al J Biol Chem. 2015 Jun 12;290(24):15004-1

Insert

ATP10D (ATP10D Human)

Tags

HA

Expression

Mammalian

Mutation

E215Q

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pDA093

Plasmid

#131390

Purpose

generation of Tet-On RPE

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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pH6HTC_HNRNPA1-Halo

Plasmid

#175307

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

HNRNPA1 (HNRNPA1 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pMX-DivisionRecorder

Plasmid

#179446

Purpose

Retroviral expression vector containing the DivisionRecorder cassette

Depositor

Ton Schumacher

Article

Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

Insert

GFP-P2A-511-CrePI-511

Use

Retroviral

Promoter

LTR

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pRSET-WHaloCaMP1a-EGFP

Plasmid

#205303

Purpose

Expression of WHaloCaMP1a-EGFP fusion in E. coli

Depositor

Eric Schreiter

Article

Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

Insert

WHaloCaMP1a-EGFP

Expression

Bacterial

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCDHblast-flx-sORF-Katushka

Plasmid

#179457

Purpose

Lentiviral vector encoding a CRE inducible Katushka gene

Depositor

Ton Schumacher

Article

Bresser et al Nat Immunol. 2022 May;23(5):791-801. doi

Insert

loxP-shuffledORF-loxP-Katushka

Use

Lentiviral

Promoter

CMV

Available Since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DD-T6B-MUT-YFP

Plasmid

#235144

Purpose

Expresses the inducible mutant T6B peptide fused with DHFR and YFP under the human synapsin promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused mutant T6B peptide

Use

AAV

Mutation

5 tryptophan residues of T6B-WT mutated

Promoter

hSyn

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-tdTomato-WPRE

Plasmid

#202610

Purpose

Double floxed red fluorescent protein tdTomato in AAV production vector expressed under the mammalian promoter (EF1a)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

tdTomato

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

EF1a

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC22A1

Plasmid

#132034

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

Insert

SLC22A1 (SLC22A1 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-hSyn-FLAG-HA-T6B-MUT-YFP

Plasmid

#235140

Purpose

Expresses the mutant T6B peptide fused with YFP under the human synapsin promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

mutant T6B peptide

Use

AAV

Tags

FLAG/HA

Mutation

5 tryptophan residues of T6B-WT mutated

Promoter

hSyn

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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