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pCMV/myc/mito-hOGG1

Plasmid

#18706

Depositor

David Sidransky

Article

Chatterjee et al BMC Cancer. 2006 Oct 3;6:235. doi: 10.11

Insert

8-oxoguanine DNA glycosylase (OGG1 Human)

Expression

Mammalian

Mutation

N/A

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pMK411 (OsTIR1(F74G) mAID-EGFP-Nluc)

Plasmid

#140659

Purpose

OsTIR1(F74G)-P2A-mAID-EGFP-Nluc

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

OsTIR1(F74G) mAID-EGFP-Nluc TOL2

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pGV15 (CK2alpha K68M)

Plasmid

#27089

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha K68M (kinase dead) (CSNK2A1 Human)

Tags

HA

Expression

Mammalian

Mutation

K68M (kinase dead)

Promoter

CMV

Available Since

May 17, 2012

Availability

Academic Institutions and Nonprofits only
ABE8.20-m

Plasmid

#136300

Purpose

expresses ABE8.20-m in mammalian cells

Depositor

Nicole Gaudelli

Article

Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

Insert

ABE8.20-m

Tags

C-terminal BPNLS

Expression

Mammalian

Mutation

D10A in S. pyogenes Cas9, TadA mutations describe…

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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FH-TET1-pEF

Plasmid

#49792

Purpose

This plasmid expresses the full-length human TET1 gene with a Flag and HA tag.

Depositor

Anjana Rao

Article

Tahiliani et al Science. 2009 May 15;324(5929):930-5. Ep

Insert

Tet methylcytosine dioxygenase 1 (TET1 Human)

Tags

Flag and HA

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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T7-VEE-CFP

Plasmid

#193357

Purpose

Self-replicating RNA vector expressing CFP fluorescent protein + PuroR

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

CFP

Use

Venezuelan equine encephalitis (vee) virus rna re…

Expression

Mammalian

Promoter

T7

Available Since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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LAMP1-mRFP-FLAG

Plasmid

#34611

Purpose

3rd gen lentiviral vector expressing a fusion protein of Lamp1, mRFP, and a FLAG tag

Depositor

David Sabatini

Article

Zoncu et al Science. 2011 Nov 4;334(6056):678-83.

Insert

LAMP1 (Lamp1 Rat)

Use

Lentiviral

Tags

2xFlag and mRFP

Expression

Mammalian

Available Since

March 20, 2012

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

Plasmid

#140018

Purpose

Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

EcoTyrRS

Tags

FLAG

Expression

Mammalian

Mutation

Y37L, D182S, F183M, L186A

Promoter

EF1

Available Since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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eTd-CBEm

Plasmid

#196602

Purpose

Mammalian expression of eTd-CBEm

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L/A48M)-nSpCas9-bpNLS-P2A-UGI-bpNLS

Tags

6*His

Expression

Mammalian

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD81-(TEV)-FKBP-SmBiT

Plasmid

#182651

Purpose

human CD81-TEV cleavage site-FKBP-SmBiT tag

Depositor

Masaharu Somiya

Article

Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

Insert

human CD81-TEVp cleavage site-FKBP-SmBiT (CD81 Human, Synthetic)

Tags

SmBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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HA-MAP1LC3B

Plasmid

#137757

Purpose

Expression of LC3B

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

MAP1LC3B (MAP1LC3B Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Mucolipin1 D471-472K-pHcRed C1

Plasmid

#62961

Purpose

full length human Mucolipin-1 D471/472K cloned into pHcRed1 C1

Depositor

Paul Luzio

Article

Pryor et al Traffic. 2006 Oct;7(10):1388-98.

Insert

Mucolipin-1 (MCOLN1 Human)

Tags

HcRed

Expression

Mammalian

Mutation

D471/472K (and two silent mutations T77 ACA inst…

Promoter

CMV

Available Since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
DPYD-FLAG

Plasmid

#61616

Purpose

The human DPYD was used to study the effect of DPYD expression on EMT

Depositor

David Sabatini

Article

Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

Insert

Dihydropyrimidine dehydrogenase (DPYD Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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HA-GABARAPL2

Plasmid

#137761

Purpose

Expression of GABARAPL2

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GABARAPL2 (GABARAPL2 Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-C-ABE8e

Plasmid

#185667

Purpose

Expresses eNme2-C-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-C

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pv6_MCPH1_BRCT W706RW815R

Plasmid

#250355

Purpose

Expresses mutant tandem BRCT domain from MCPH1 fused to eGFP. Serves as control.

Depositor

Tuncay Baubec

Article

da Silva et al Nat Commun. 2025 Nov 20;16(1):10127. doi

Insert

MCPH1 tandem BRCT

Tags

NLS, eGFP and biotintag, TEV cleavage

Expression

Mammalian

Mutation

W706R and W815R

Promoter

CAGGS

Available Since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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VP12

Plasmid

#72247

Purpose

Human expression plasmid for SpCas9-HF1 variant: CMV-T7-humanSpCas9-HF1(N497A, R661A, Q695A, Q926A)-NLS-3xFLAG

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

Insert

mammalian codon-optimized Streptococcus pyogenes Cas9 HF1(N497A/R661A/Q695A/Q926A)-NLS-3xFlag

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Mammalian

Mutation

N497A, R661A, Q695A and Q926A

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pJC49

Plasmid

#133060

Purpose

Lentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.

Depositor

Martin Kampmann

Article

Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

Insert

Tau.K18(LM)

Use

Lentiviral

Tags

mScarletI

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pREP-8xARE-GFP-SV40-BFP

Plasmid

#134910

Purpose

NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

Depositor

Markus Landthaler

Article

Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

Inserts

8xARE- minimal promoter - GFP
TagBFP

Expression

Mammalian

Promoter

8xARE - minimal promoter and SV40

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pSI-Target-AID-NG

Plasmid

#119861

Purpose

Expresses Target-AID-NG (NLS-nSpCas9-NG-NLS-SH3-3xFLAG-PmCDA1-NLS-UGI) in mammalian cells.

Depositor

Osamu Nureki

Article

Nishimasu et al Science. 2018 Aug 30. pii: science.aas91

Insert

nSpCas9-NG (D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R)

Expression

Mammalian

Available Since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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