Addgene

We narrowed to 4,413 results for: Ado;

Showing: 1501 - 1530 of 4413 results

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N-TAP A2A-YFP pcDNA3

Plasmid

#67852

Purpose

mammalian expression of A2A receptor with N terminal TAP tag and C terminal YFP fusion

Depositor

Christian Gruber

Article

Bergmayr et al J Biol Chem. 2013 Oct 4;288(40):28831-44

Insert

adenosine A2a receptor (ADORA2A Human)

Use

Tags

TAP and YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2015

Availability

Academic Institutions and Nonprofits only
pSP64T acvr1ba*

Plasmid

#185409

Purpose

Constitutively active form of acvr1ba (Nodal receptor). For in vitro transcription (SP6)

Depositor

Nicolas David

Article

Boutillon et al Dev Cell. 2022 May 17. pii: S1534-5807(2

Insert

acvr1ba (acvr1ba Zebrafish)

Use

In vitro synthesis of mrna

Tags

Expression

Mammalian

Mutation

changed Threonine 206 to Aspartic acid

Promoter

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti GW-mCit-PA

Plasmid

#113457

Purpose

Lentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral

Tags

mCitrine-ProteinA

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-GFP-RELA K5R

Plasmid

#196631

Purpose

Express GFP-RELA K5R

Depositor

Nicolas Manel

Article

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

GFP-RELA K5R (RELA Human)

Use

Lentiviral

Tags

GFP

Expression

Mutation

K122R, K123R, K310R, K314R, K315R

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEE038

Plasmid

#176795

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

407

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE048

Plasmid

#176805

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

504

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti GW-NL-myc

Plasmid

#113455

Purpose

Lentiviral NanoLuc Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral and Luciferase

Tags

NanoLuc-cmyc

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF14_P2A_Hygro_Barcode

Plasmid

#120500

Purpose

Barcoded lentiviral vector to express KLF14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF14 (KLF14 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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3788 pmko-neo A9-1

Plasmid

#8878

Depositor

Stanley Korsmeyer

Article

Wang et al EMBO J. 2005 Jan 26. 24(2):368-81.

Insert

Hox A9 (HOXA9 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
3788 pmko-neo A9-3

Plasmid

#8880

Depositor

Stanley Korsmeyer

Article

Wang et al EMBO J. 2005 Jan 26. 24(2):368-81.

Insert

Hox A9 (HOXA9 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only
pFETCh_FOXM1_iso1

Plasmid

#135734

Purpose

Donor vector for 3' FLAG tag of human FOXM1_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

FOXM1_iso1 Homology arms (FOXM1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_FOXM1_iso1_2

Plasmid

#135753

Purpose

Encodes gRNA for 3' target of human FOXM1_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

FOXM1_iso1 gRNA (FOXM1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_FOXM1_iso1_1

Plasmid

#135752

Purpose

Encodes gRNA for 3' target of human FOXM1_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

FOXM1_iso1 gRNA (FOXM1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-NL

Plasmid

#113445

Purpose

ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-NanoLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-NL

Plasmid

#113444

Purpose

ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine-NanoLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAC148-pCR8-dCas9VP96

Plasmid

#48220

Purpose

dCas9VP96 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expression

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP96 activation domain

Use

CRISPR; Gateway donor

Tags

HA Tag and VP96

Expression

Mutation

D10A;H840A

Promoter

Available since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pLenti PA-mCit-GW

Plasmid

#113456

Purpose

Lentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral

Tags

ProteinA-mCitrine

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pFETCh_ARID4B

Plasmid

#101072

Purpose

Donor vector for 3' FLAG tag of human ARID4B

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ARID4B homology arms (ARID4B Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_GABPB1_iso2_1

Plasmid

#104041

Purpose

Encodes gRNA for 3' target of human GABPB1_iso2 along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

GABPB1_iso2 gRNA (GABPB1 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_GABPB1_iso2

Plasmid

#104035

Purpose

Donor vector for 3' FLAG tag of human GABPB1_iso2

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

GABPB1_iso2 homology arms (GABPB1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti PA-NL

Plasmid

#113453

Purpose

Lentiviral ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-NanoLuc

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti PA-mCit

Plasmid

#113451

Purpose

Lentiviral ProteinA-mCitrine expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUBbC

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pFETCh_RERE

Plasmid

#101069

Purpose

Donor vector for 3' FLAG tag of human RERE

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

RERE homology arms (RERE Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_RERE_1

Plasmid

#101073

Purpose

Encodes gRNA for 3' target of human RERE

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against RERE (RERE Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_ASCL4_P2A_Hygro_Barcode

Plasmid

#120429

Purpose

Barcoded lentiviral vector to express ASCL4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

ASCL4 (ASCL4 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pN-iRS3GG

Plasmid

#98858

Purpose

Expresses pheS cassette (in place of an antisense RNA probe, for golden gate cloning) in E. coli (kanR).

Depositor

Lydia Contreras

Article

Vazquez-Anderson et al Nucleic Acids Res. 2017 May 19;45(9):552

Insert

PheS cassette to be selectively replaced by unique asRNA probes

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-BE3-PGK-Puro

Plasmid

#110842

Purpose

Lentiviral vector for constitutive expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Tags

Expression

Mutation

D10A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-BE3-P2A-Puro

Plasmid

#110838

Purpose

Lentiviral vector for constitutive expression of BE3 (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3

Use

Lentiviral

Tags

Expression

Mutation

D10A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-BE3-FNLS

Plasmid

#110836

Purpose

CMV expression vector for BE3-FNLS construct (not codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3-FNLS

Use

Tags

3X FLAG

Expression

Mammalian

Mutation

NLS sequence at the N-terminus

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-v1-sgDHFR

Plasmid

#233898

Purpose

lentiviral vector expressing Cas9 and an sgRNA targeting DHFR

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

sgRNA targeting DHFR (DHFR Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge