We narrowed to 22,480 results for: cas9 genes

1. pMSCVpuro-Cas9-EGFP

   Plasmid

   #159126

   Purpose

   Expresses active Cas9 fused with EGFP for fluorescence microscopy

   Depositor

   Taekjip Ha

   Article

   Liu et al Science. 2020 Jun 12;368(6496):1265-1269

   Insert

   Cas9 fused to EGFP
   

   Use

   CRISPR and Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR promoter

   Available since

   Dec. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-RSV-SpCas9

   Plasmid

   #85450

   Purpose

   Express SpCas9 in mammalian cells

   Depositor

   Hetian Lei

   Article

   Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

   Insert

   pRSV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pRSV

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW-Cas9-2A-EGFP

   Plasmid

   #167928

   Purpose

   Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Insert

   T2A-EGFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tTRE, hPGK

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW-Cas9-Blast

   Plasmid

   #83481

   Purpose

   Lentiviral vector for mammalian expression of doxycycline-inducible Cas9 and constitutive expression of Blasticidin S deaminase -T2A -rtTA

   Depositor

   Mohan Babu

   Inserts

   SpCas9
   Blasticidin S deaminase -T2A - reverse tetracycline-controlled transactivator
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   Tight Tre and human phosphoglycerate kinase 1 pro…

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKLV2-EF1a-Cas9Bsd-W

   Plasmid

   #68343

   Purpose

   Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-Cas9 cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Cas9 fused with Flag-tag and a NLS at the N termi…

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-SpCas9 neo

   Plasmid

   #104996

   Purpose

   This lentiviral construct delivers hSpCas9 and G418 resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. lenti-SpCas9 hygro

   Plasmid

   #104995

   Purpose

   This lentiviral construct delivers hSpCas9 and hygromycin resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV_dCas9-ZIM3-KRAB_sgTAOK2i_#3

   Plasmid

   #172985

   Purpose

   CRISPRi for TAOK2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   sgTAOK2i (TAOK2 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   Available since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EFS-SpCas9

   Plasmid

   #104588

   Purpose

   AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

   Depositor

   Ryohei Yasuda

   Article

   Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

   Insert

   Streptococcus pyogenes Cas9
   

   Use

   AAV and CRISPR

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-TRE-dCas9-VPR

    Plasmid

    #63800

    Purpose

    SP-dCas9-VPR with doxycycline-inducible expression

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

    Insert

    dCas9-VPR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDT-SuperFi-Cas9

    Plasmid

    #183630

    Purpose

    Expression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coli

    Depositor

    David Taylor

    Article

    Bravo et al Nature. 2022 Mar 2. pii: 10.1038/s41586-

    Insert

    SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …

    Promoter

    T7

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9n(BB)-2A-GFP (PX461)

    Plasmid

    #48140

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9-DNMT3A-PuroR

    Plasmid

    #71667

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, S. pyogenes, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-Cas9-His

    Plasmid

    #98158

    Purpose

    Purification of Cas9 protein

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Commun. 2017 Jan 18;8:14261. doi: 10

    Insert

    NLS-Cas9-NLS
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid

    #113341

    Purpose

    Expresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

    Depositor

    Gavin Wright

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    VP64-dCas9-VP64-T2A-MS2-p65-HSF1
    

    Use

    Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpCas9-NG-ABE8e(V106W)

    Plasmid

    #198533

    Purpose

    Expresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSD

    Depositor

    Hyongbum Kim

    Article

    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

    Insert

    SpCas9-NG-ABE8e(V106W)
    

    Use

    CRISPR and Lentiviral

    Tags

    BSD, FLAG, and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pdCas9-sgRNA-RFP

    Plasmid

    #166005

    Purpose

    Bacterial expression of dCas9 (aTc control) and sgRNA (arabinose control)

    Depositor

    Martin Buck

    Article

    Bradley et al Biotechnol Rep (Amst). 2021 Oct 9;32:e00

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. p-dCas9-JMJD2A-Hygro

    Plasmid

    #104410

    Purpose

    transient expression of dCas9-JMJD2A fusion protein

    Depositor

    Stephan Beck

    Insert

    JMJD2A (KDM4A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-Hs.cargo(Cas9)

    Plasmid

    #174864

    Purpose

    Expresses human Peg10 cargoRNA encoding SpCas9

    Depositor

    Feng Zhang

    Article

    Segel et al Science. 2021 Aug 20;373(6557):882-889.

    Insert

    SpCas9 flanked by HsPeg10 UTRs
    

    Use

    Tags

    HA-SV40 NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-sadCas9-VP64

    Plasmid

    #115790

    Purpose

    An AAV vector expressing S. aureus dCas9 fused to VP64.

    Depositor

    Nadav Ahituv

    Article

    Matharu et al Science. 2018 Dec 13. pii: science.aau06

    Insert

    Sa-dCas9
    

    Use

    AAV

    Tags

    NLS and NLS-VP64

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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