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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,770 results for: Nes

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Showing: 10381 - 10400 of 15770 results

  4. Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

    Plasmid
    #157984
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    hU6
    Available Since
    March 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    157984-plasmid-map-sequence-id-355759

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  7. pGG426_UV5

    Plasmid
    #165608
    Purpose
    Vector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)
    Depositor
    Marcus Noyes
    Article
    Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:
    Insert
    lacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    D1135V, S1136R, D1332K and R1333G mutations in Sp…
    Promoter
    lacUV5 driving Cas9 VRKG and UV5 driving sgRNA
    Available Since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    165608-plasmid-map-sequence-id-330965

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  8. pRS405 PEX14-VAC8-RFP

    Plasmid
    #166844
    Purpose
    Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554
    Insert
    VAC8 (VAC8, PEX14 Budding Yeast)
    Tags
    PEX14 transmembrane domain and RFP
    Expression
    Yeast
    Mutation
    PEX14 transmembrane domain
    Promoter
    VAC8 promoter
    Available Since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166844-plasmid-map-sequence-id-329332

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  11. pCFJ2306 - [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid
    #159848
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - ceGFP(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    Expression
    Worm
    Mutation
    Not applicable
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159848-plasmid-map-sequence-id-318006

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  12. pCFJ2307 - [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)

    Plasmid
    #159856
    Purpose
    Three-fragment Gateway compatible flourophore for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Fluor - ce-tagRFP-T(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
    Expression
    Worm
    Mutation
    Not applicable
    Available Since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159856-plasmid-map-sequence-id-318019

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  14. Mouse 5' Srcap AID GFP Puro

    Plasmid
    #127903
    Purpose
    HDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Srcap Gene
    Depositor
    Mark Groudine
    Insert
    Srcap (Srcap Mustard Weed, Mouse)
    Use
    Donor plasmid
    Tags
    GFP, AID, PuroR
    Available Since
    Sept. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    127903-plasmid-map-sequence-id-251069

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  15. pAAV-hSyn-iLMO (slGLuc-Mac-EYFP)

    Plasmid
    #114100
    Purpose
    fusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11
    Insert
    Gaussia luciferase, superluminescent; Leptosphaeria maculans opsin; EYFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    114100-plasmid-map-sequence-id-252513

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  17. pRRLSIN.cPPT.PGK.MD(ND2bHLH)WCS.WPRE

    Plasmid
    #122056
    Purpose
    Lentiviral expression of a MyoD chimeric protein substituted with the NeuroD2 bHLH domain and point mutations to prevent MyoD interaction with PBX/MEIS: W96A, C98A, and S253P
    Depositor
    Stephen Tapscott
    Article
    Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do
    Insert
    MD(ND2bHLH)WCS (Myod1, Neurod2 Mouse)
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    MyoD chimeric protein substituted with the NeuroD…
    Available Since
    Feb. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    122056-plasmid-map-sequence-id-235399

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  19. pdCas9-M-3F2

    Plasmid
    #73428
    Purpose
    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant 3F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Repressor C4 (orthogonal T7-lac repressor)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    Constitutive wild-type S. pyogenes promoter
    Available Since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73428-plasmid-map-sequence-id-135328

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Showing: 10381 - 10400 of 15770 results