167,948 results

1. pENN.AAV.tMCK.PI.eGFP.WPRE.bGH

   Plasmid

   #105556

   Purpose

   AAV expression of EGFP from tMCK promoter

   Depositor

   James M. Wilson

   Has Service

   AAV9

   Insert

   EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   tMCK

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLentiRhoA2G

   Plasmid

   #40179

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   RhoA Biosensor
   

   Use

   Lentiviral

   Tags

   His-Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. p(HA)HIF1alpha (P402A,P564A)

   Plasmid

   #52636

   Purpose

   mammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutation

   Depositor

   Eric Huang

   Article

   Kageyama et al FASEB J. 2004 Jun;18(9):1028-30. Epub 20

   Insert

   HIF1alpha P402A, P564A (HIF1A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   P402A, P564A

   Promoter

   CMV

   Available Since

   April 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-BAF

   Plasmid

   #101772

   Purpose

   Expresses EGFP tagged BAF in human cells

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. CIp10 - γmGFP (γmGFP based)

   Plasmid

   #163119

   Purpose

   Expresses in Candida albicans

   Depositor

   Patrick Van Dijck

   Article

   Van Genechten et al Sci Rep. 2020 Apr 9;10(1):6158. doi: 10.

   Insert

   gamma mGFP
   

   Use

   Candida albicans expression

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

   Plasmid

   #234454

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-NGR
   

   Use

   AAV; Flp/frt

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmCherry-RIAM

   Plasmid

   #80030

   Purpose

   Mammalian expression of mCherry-RIAM (FL)

   Depositor

   Chinten James Lim

   Insert

   RIAM (APBB1IP Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

8. p3XFlag-CAPN2

   Plasmid

   #60942

   Purpose

   Expresses calpain2 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Calpain 2 (CAPN2 Human)
   

   Tags

   3xflag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-PH-PLC-delta-EGFP

   Plasmid

   #136998

   Purpose

   Lentiviral EGFP lipid sensor for PI(4,5)P2

   Depositor

   Ken-Ichi Takemaru

   Insert

   PH-PLC-delta-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Promoter

   EF1 alpha

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. OmpA3-PNGaseF-TEV-His6

    Plasmid

    #114274

    Purpose

    Amidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.

    Depositor

    Martin Caffrey

    Insert

    Peptide: N-glycosidase F
    

    Tags

    OmpA3 leader sequence (for periplasmic localisati…

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMaRSC

    Plasmid

    #89189

    Purpose

    The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.

    Depositor

    David Tirrell

    Article

    Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

    Insert

    Flag-L274G-T2A-mCherry (Mars1 Mouse)
    

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas9-10xSunTag-P2A-tagBFP

    Plasmid

    #174141

    Purpose

    Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, 10xSunTag, tagBFP
    

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. p663-UBC-V5-BRSK1_IDG-K

    Plasmid

    #135270

    Purpose

    Gateway destination clone of BRSK1 (human) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    V5-BRSK1 (BRSK1 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    Jan. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. BL21∆serB

    Bacterial Strain

    #34929

    Depositor

    Jesse Rinehart, Dieter Söll

    Article

    Park et al Science. 2011 Aug 26;333(6046):1151-4.

    Bacterial Resistance

    None

    Available Since

    Feb. 24, 2012

    Availability

    Academic Institutions and Nonprofits only

15. TFORF2258

    Plasmid

    #142927

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    SOX11 (SOX11 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid

    #104911

    Purpose

    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

    Depositor

    Brigitte Gasser

    Article

    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

    Inserts

    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MEK1C222

    Plasmid

    #164638

    Purpose

    Bacterial expression plasmid for His6-MEK1C222

    Depositor

    Ben Davis

    Article

    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

    Insert

    mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    

    Tags

    His6-tag

    Expression

    Bacterial

    Mutation

    G(-19)F/S222C/C277S/C376S

    Promoter

    T7

    Available Since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSET-4xTR-TUBE

    Plasmid

    #110312

    Purpose

    E. coli expression and purification of 4xTR-TUBE

    Depositor

    Yasushi Saeki

    Article

    Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

    Insert

    4xTR-TUBE (UBQLN1 Human)
    

    Tags

    N-terminal His6-T7 tag

    Expression

    Bacterial

    Mutation

    All Arg residues in the UBA domain are mutated to…

    Promoter

    T7

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pScaf-1512.1

    Plasmid

    #111402

    Purpose

    pScaf phagemid with insert for generating DNA origami scaffold 1512 bases in length

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Insert

    pScaf-1512
    

    Expression

    Bacterial

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBAD-HisD-KRaION1

    Plasmid

    #177799

    Purpose

    Bacterial expression of green fluorescent potassium indicator KRaION1

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION1
    

    Expression

    Bacterial

    Promoter

    araB

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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