We narrowed to 29,674 results for: REP

1. PME-1 S156A (inakt.mut) pcDNA-StrepIII

   Plasmid

   #119293

   Purpose

   Mammalian overexpression of StrepII-tagged full length PPME1 with mutation in the methylesterase active center

   Depositor

   Jukka Westermarck

   Insert

   PPME1 (PPME1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   S156A

   Promoter

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. SEAP (Protein secretion reporter) in pLX317

   Plasmid

   #233290

   Purpose

   SEAP reporter (used for monitoring protein secretion)

   Depositor

   Sefi Rosenbluh

   Insert

   SEAP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   E1Fa

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCT376: pTwinStrep-bdSUMO-ZooTsy_TnsF-Y584F

   Plasmid

   #200877

   Purpose

   ZooTsy_TnsF expression construct for protein purification.

   Depositor

   Feng Zhang

   Article

   Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

   Insert

   ZooTsy_TnsF
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   TnsF-Y584F

   Promoter

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCT375: pDonor(ZooTsy)_excision-reporter

   Plasmid

   #200873

   Purpose

   Reporter for ZooTsy excision.

   Depositor

   Feng Zhang

   Article

   Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

   Insert

   ZooTsy
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-Twin-strep-TEV-DGKδ2

   Plasmid

   #223720

   Purpose

   Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   DGKD (DGKD Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted start codon

   Promoter

   CAG and chicken β-actin promoter

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS-PHOSPHO2-TEV-Twin-Strep

   Plasmid

   #222016

   Purpose

   Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   PHOSPHO2 (PHOSPHO2 Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted stop codon (TAA)

   Promoter

   chicken β-actin promoter

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGGS-PHOSPHO1-TEV-Twin-Strep

   Plasmid

   #222015

   Purpose

   Express human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   PHOSPHO1 (PHOSPHO1, PHOSPHO1 Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   stop codon (TGA) is deleted

   Promoter

   chicken β-actin promoter

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX-PHOSPHO2-TEV-Twin-Strep

   Plasmid

   #222017

   Purpose

   Express human PHOSPHO2 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in E. coli

   Depositor

   Chiaki Murakami

   Insert

   PHOSPHO2 (PHOSPHO2 Human)
   

   Use

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Bacterial

   Mutation

   deleted stop codon (TAA)

   Promoter

   lac promoter

   Available since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pACEBac1_Strep-Psc-BICD2(711-800)

   Plasmid

   #228833

   Purpose

   Insect cell expression of CC3 domain of BICD2 (aa 711-800)

   Depositor

   Andrew Carter

   Article

   Manigrasso et al bioRxiv 2024.04.11.589026

   Insert

   BICD2 (Bicd2 Mouse)
   

   Use

   Tags

   Strep

   Expression

   Insect

   Mutation

   aa 711-800

   Promoter

   Available since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pACEBac1_Strep-Psc-BICD2(342-800)

    Plasmid

    #228832

    Purpose

    Insect cell expression of C-terminal portion of BICD2 (aa 342-800)

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    BICD2 (Bicd2 Mouse)
    

    Use

    Tags

    Strep

    Expression

    Insect

    Mutation

    aa 342-800

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPICZ A-Strep-Opa1(MGD) (SB252)

    Plasmid

    #227601

    Purpose

    Inducible expression of Strep-tagged Opa1(MGD) in Pichia pastoris

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    OPA1 (OPA1 Human)
    

    Use

    Tags

    Twin-StrepTag

    Expression

    Yeast

    Mutation

    contains aa 253-580 (MGD) and aa 938-960

    Promoter

    AOX1

    Available since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBIG1a_StrepII-Hook3[451-718]_FTS_FHIP1B

    Plasmid

    #222300

    Purpose

    Co-expresses truncated human FHF (FTS, StrepII-HOOK3[451-718] and FHIP1B) in a pBIG1a vector

    Depositor

    Andrew Carter

    Article

    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

    Inserts

    HOOK3[451-718] (HOOK3 Human)
    FTS (AKTIP Human)
    FHIP1B (FHIP1B Human)
    

    Use

    Tags

    StrepII-Psc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBIG1a_StrepII-Hook3[571-718]_FTS_FHIP1B

    Plasmid

    #222301

    Purpose

    Co-expresses truncated human FHF (FTS, StrepII-HOOK3[571-718] and FHIP1B) in a pBIG1a vector

    Depositor

    Andrew Carter

    Article

    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

    Inserts

    HOOK3[571-718] (HOOK3 Human)
    FTS (AKTIP Human)
    FHIP1B (FHIP1B Human)
    

    Use

    Tags

    StrepII-Psc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPPI4-Strep-SNAP-CD80-His10

    Plasmid

    #223579

    Purpose

    Protein purification of human CD80 with Twin-Strep, SNAP and 10xHis tags from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

    Insert

    CD80 (CD80 Human)
    

    Use

    Tags

    His10 and Twin-Strep, SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPPI4-Strep-SNAP-CD86-His10

    Plasmid

    #223570

    Purpose

    Protein purification of human CD86 with Twin-Strep, SNAP and 10xHis tags from mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

    Insert

    CD86 (CD86 Human)
    

    Use

    Tags

    His6 and Twin-Strep, SNAP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-HsProm1s1(W795R)-TwinStrep-His

    Plasmid

    #210817

    Purpose

    Mammalian overexpression vector for C-terminally TwinStrep and His tagged human Prom1s1 (W795R)

    Depositor

    Luke Chao

    Article

    Bell et al Elife. 2024 Aug 13;13:e100061. doi: 10.7

    Insert

    Prominin 1 (PROM1 Human)
    

    Use

    Tags

    TwinStrep, 10xHis

    Expression

    Mammalian

    Mutation

    Changed Trp-795 to Arg

    Promoter

    CMV

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJS-BCD-Strep-SAVED-CHAT

    Plasmid

    #214030

    Purpose

    Bacterial expression plasmid for strep-tagged wild-type SAVED-CHAT from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJS-BCD-Strep-dSAVED-CHAT

    Plasmid

    #214031

    Purpose

    Bacterial expression plasmid for strep-tagged catalytically dead SAVED-CHAT mutant from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    H375A, C422A

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJS-BCD-Strep-SAVED-CHAT-sm

    Plasmid

    #214033

    Purpose

    Bacterial expression plasmid for strep-tagged SAVED-CHAT CHAT-CHAT singlet interface mutant from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    SAVED-CHAT
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    R456E, D459K, D476K, R479E

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJS-BCD-Strep-PCaspase-R153A

    Plasmid

    #214036

    Purpose

    Bacterial expression plasmid for strep-tagged PCaspase (Prokaryotic Caspase) R153A mutant from Haliangium ochraceum

    Depositor

    Raymond Staals

    Article

    Steens et al Science. 2024 Feb 2;383(6682):512-519. d

    Insert

    PCaspase
    

    Use

    Tags

    Strep-tag II

    Expression

    Bacterial

    Mutation

    R153A

    Promoter

    T7 promoter

    Available since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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