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Viral Prep#213940-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiPVe3_dTomato_nlsdTomato (#213940). In addition to the viral particles, you will also receive purified pAAV_BiPVe3_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the PV+ basket cell-targeting enhancer E3. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only
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pAAV_BiCHATe27_dTomato_nlsdTomato (AAV PHP.eB)
Viral Prep#213829-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiCHATe27_dTomato_nlsdTomato (#213829). In addition to the viral particles, you will also receive purified pAAV_BiCHATe27_dTomato_nlsdTomato plasmid DNA. Bicistronic expression of dTomato and nuclear-targeted dTomato under the control of the cholinergic neuron-targeting enhancer E27. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsdTomatoAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiLAMP5e3_ChR2_mCherry (AAV PHP.eB)
Viral Prep#213915-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiLAMP5e3_ChR2_mCherry (#213915). In addition to the viral particles, you will also receive purified pAAV_BiLAMP5e3_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the Lamp5 interneuron-targeting enhancer E3. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsmCherryAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiCHATe27_ChR2_mCherry (AAV PHP.eB)
Viral Prep#213830-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiCHATe27_ChR2_mCherry (#213830). In addition to the viral particles, you will also receive purified pAAV_BiCHATe27_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the cholinergic neuron-targeting enhancer E27. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsmCherryAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV_BiPVe4_ChR2_mCherry (AAV PHP.eB)
Viral Prep#213937-PHPeBPurposeReady-to-use AAV PHP.eB particles produced from pAAV_BiPVe4_ChR2_mCherry (#213937). In addition to the viral particles, you will also receive purified pAAV_BiPVe4_ChR2_mCherry plasmid DNA. Expression of mCherry-tagged channelrhodopsin-2 under the control of the chandelier cell-targeting enhancer E4. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DepositorPromoterTagsmCherryAvailable sinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
Zinc Finger Consortium OPEN Accessory Reagent Set
Plasmid Kit#1000000013PurposeContains plasmids and strains used to engineer Zinc Finger Nucleases (ZFNs) via the Oligomerized Pool ENgineering (OPEN) method.DepositorAvailable sinceJuly 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ihSyn1-tTA (AAV1)
Viral Prep#99120-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-ihSyn1-tTA (#99120). In addition to the viral particles, you will also receive purified pAAV-ihSyn1-tTA plasmid DNA. Expression of the tet-off transactivator from an inducible synapsin promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterihSyn1TagsNoneAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ihSyn1-DIO-tTA (AAV1)
Viral Prep#99121-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-ihSyn1-DIO-tTA (#99121). In addition to the viral particles, you will also receive purified pAAV-ihSyn1-DIO-tTA plasmid DNA. Cre-dependent expression of the tet-off transactivator from an inducible synapsin promoter. These AAV preparations are suitable purity for injection into animals.DepositorPromoterihSyn1TagsNoneAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag (AAV5)
Viral Prep#209655-AAV5PurposeReady-to-use AAV5 particles produced from pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag (#209655). In addition to the viral particles, you will also receive purified pAAV.CAGFLEX.(cyto).iATPSnFR2.S29W.A95K.HaloTag plasmid DNA. CAG-driven, Cre-dependent expression of the high affinity ATP biosensor iATPSnFR2. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (Lentiviral Prep)
Viral Prep#92379-LVPurposeReady-to-use Lentiviral Prep particles produced from Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (#92379). In addition to the viral particles, you will also receive purified Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR activation screening in human cells. Contains 56,762 sgRNAs, targeting 18,885 genes. Concentrated lentiviral particles carrying set A of the Calabrese human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) .DepositorPromoterTagsNoneAvailable sinceAug. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-Fluc-CG3048_A
Plasmid#145856PurposeInsect Expression of CG3048-3UTRDepositorInsertCG3048-3UTR (Traf4 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP2_leu2d_NRT1.1
Plasmid#58332PurposeExpresses Arabidopsis thaliana NRT1.1 in Saccharomyces cells as a C-terminal GFP his tagged proteinDepositorInsertNRT1.1 (NRT1.1 Mustard Weed)
UseTagsGFP-HisExpressionYeastMutationPromoterGAL1Available sinceAug. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
Target Accelerator Pan-Cancer Mutant Collection
Plasmid Kit#1000000103PurposePanel of cancer alleles that have undergone basic, functional screening; full-length, human clones (454 mutant and 177 corresponding wild-type) in the Gateway cloning systemDepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBB328
Plasmid#221533PurposePlasmid for the introduction of Mariner transposon that inserts deoxyviolacein cassette behind indigenous promoters in target bacterial strain. Plasmid has tetracycline marker for selection.DepositorInsertsvioABCE
Mariner transposase and transposon backbone from pBB298 (originally from pEB001)
Tetracycline antibiotic cassette from pRK415
UseTagsExpressionBacterialMutationPromoterNone, contains RBS siteAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dat - EGFP - Tol2
Plasmid#85418Purposefor making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) geneDepositorInsertdopamine transporter (slc6a3 Zebrafish)
UseZebrafish transgenesisTagsEGFPExpressionMutationPromoterdatAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV/N3IC-HA
Plasmid#47618Purposein vitro transcription and translation of HA-tagged Notch3 Intracellular domainDepositorInsertNotch3 Intracellular domain (Notch3 Mouse)
UseIn vitro transcription and translationTagsHAExpressionMammalianMutationcodons 1664 (Met) to 2318 (Ala)PromoterCMVAvailable sinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
PB-SRT-Puro_BC4
Plasmid#193146PurposeBarcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cardsDepositorInsertBarcoded piggyBac Puro SRT
UseTagsExpressionMammalianMutationBarcode: CAACPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-SRT-Puro_BC23
Plasmid#193165PurposeBarcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cardsDepositorInsertBarcoded piggyBac Puro SRT
UseTagsExpressionMammalianMutationBarcode: CTAGPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-SRT-Puro_BC22
Plasmid#193164PurposeBarcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cardsDepositorInsertBarcoded piggyBac Puro SRT
UseTagsExpressionMammalianMutationBarcode: CAGTPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-SRT-Puro_BC21
Plasmid#193163PurposeBarcoded piggyBac self-reporting transposon with puromycin marker for mammalian calling cardsDepositorInsertBarcoded piggyBac Puro SRT
UseTagsExpressionMammalianMutationBarcode: TTGGPromoterAvailable sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only