We narrowed to 34,020 results for: STO

Showing: 10621 - 10640 of 34020 results

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gRNA NIA Null

Plasmid

#198728

Purpose

Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Neither gRNA is fused to a mobility signal and are therefore non-mobile.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

AtNIA1 gRNAs

Expression

Plant

Promoter

pU6-26, pU6-29

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pKB238

Plasmid

#170033

Purpose

Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Neuropeptide Y (NPY Escherichia coli, Human, Synthetic)

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Promoter

Ptac and PvirK

Available Since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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STAGR_gRNAScaffold_mU6

Plasmid

#102844

Purpose

Can be used as PCR template for a STAgR reaction

Depositor

Stefan Stricker

Article

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Insert

STAgR Insert gRNAScaffold_mU6

Use

Pcr template for stagr inserts

Promoter

mU6

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pKD4-Nter-APEX2

Plasmid

#112868

Purpose

Bacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner method

Depositor

Eric Cascales

Article

Santin et al Nat Microbiol. 2018 Nov;3(11):1304-1313.

Insert

APEX2

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-KSENB-Nup133

Plasmid

#87340

Purpose

To express human Nup133 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

NUP133 (NUP133 Human)

Tags

EGFP

Expression

Mammalian

Mutation

deletion of Nup133 AA1-7

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-U6sgp53-U6sgbbSapI-GFAPCre

Plasmid

#100275

Purpose

AAV-CRISPR mediated genome editing in glia / astrocytes

Depositor

Sidi Chen

Article

Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

Type

Empty backbone

Use

AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

Expression

Mammalian

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pMMLV-mRFP1-P2A-Puro

Plasmid

#160228

Purpose

Expresses mRFP in mammalian cells

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

mRFP1-P2A-Puro

Use

Retroviral

Expression

Bacterial and Mammalian

Promoter

LTR

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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CAV1-deltaN-mEGFP

Plasmid

#27707

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

caveolin-1, N-terminal truncated (60-178) (CAV1 Canis lupus familiaris)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

N-terminal truncation with AA 1-59 missing. The r…

Available Since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

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pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)

Plasmid

#86414

Purpose

nuclear split GFP11 reporter without mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-GFP11-PUS1

Tags

GFP11 and mCherry

Expression

Yeast

Promoter

NOP1pr

Available Since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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BB1_12_pAOX1

Plasmid

#98514

Purpose

BB1 with insert: promoter PAOX1

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Insert

AOX1 promoter

Expression

Bacterial

Mutation

Internal BsaI and BpiI sites removed from insert

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a Gx-NL

Plasmid

#169105

Purpose

Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.

Depositor

Maarten Merkx

Article

Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66

Insert

protein G - NanoLuc

Tags

his-tag and strep-tag

Expression

Bacterial

Mutation

Ala 24 stop

Promoter

T7

Available Since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

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mH2A1.2-CT-GFP

Plasmid

#45169

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy (MACROH2A1 Human)

Tags

GFP

Expression

Mammalian

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 IL6

Plasmid

#67061

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL6 (IL6 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pBad-CA TAG126

Plasmid

#105836

Purpose

Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 126 and C-terminal 6x his tag

Depositor

Ryan Mehl

Article

Mehl et al 2020

Insert

human carbonic anhydrase II thermostable variant (CA2 Human)

Tags

6x his tag

Expression

Bacterial

Mutation

TAG stop codon at site 126

Promoter

pBad/arabinose

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pre_IV_CS10_1033_v1

Plasmid

#126860

Purpose

4x 1033 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubation

Depositor

Hendrik Dietz

Article

Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

Insert

IV_CS10_1033_v1

Use

Synthetic Biology

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pDx_mCherry-SYFP2

Plasmid

#189759

Purpose

Dual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mCherry-SYFP2

Tags

6xHis

Expression

Bacterial and Mammalian

Promoter

CMV & Rha

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG HDAC5 WT

Plasmid

#32213

Depositor

Reuben Shaw

Article

Mihaylova et al Cell. 2011 May 13;145(4):607-21.

Insert

HDAC5 (HDAC5 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2012

Availability

Academic Institutions and Nonprofits only
MSCVhygro-F-Ezh2-F667I

Plasmid

#24927

Depositor

Kai Ge

Article

Wang et al Proc Natl Acad Sci U S A. 2010 Apr 20. 1

Insert

Ezh2 (EZH2 Mouse)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Phenylanaline(F)667 mutated to Isoleucine(I)

Available Since

July 15, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT/TO-Flag-RNF169

Plasmid

#74243

Purpose

Expresses RNF169 in mammalian cells

Depositor

Daniel Durocher

Article

Panier et al Mol Cell. 2012 Aug 10;47(3):383-95. doi:

Insert

RNF169 (RNF169 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

April 20, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pMMLV-EBFP-P2A-Puro

Plasmid

#160227

Purpose

Expresses EBFP in mammalian cells

Depositor

Jason Sheltzer

Article

Lukow et al Dev Cell. 2021 Sep 13;56(17):2427-2439.e

Insert

EBFP-P2A-Puro

Use

Retroviral

Expression

Bacterial and Mammalian

Promoter

LTR

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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