-
-
rEc.13
Bacterial Strain#69494PurposeRecoded strain from MG1655 with prfADepositorBacterial ResistanceAmpicillinAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-C2-hSphK2
Plasmid#84370PurposeExpression of AcGFP-SphK2 fusion protein in mammalian cellsDepositorInsertSPHK2 (SPHK2 Human)
UseTagsAcGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
ATMY3
Bacterial Strain#218771PurposeE. coli strain, genotype: EcNR1GT pUltraBR MjY dtyrS tyrTV::CUA tyrU::inact lambda::tolCDepositorBacterial ResistanceSpectinomycinSpeciesEscherichia coliAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATMY4
Bacterial Strain#218772PurposeE. coli strain, genotype: EcNR1GT pUltraBR MjY dtyrS tyrTV::CUA tyrU::CUA lambda::tolCDepositorBacterial ResistanceSpectinomycinSpeciesEscherichia coliAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATMY5
Bacterial Strain#218773PurposeE. coli strain, genotype: EcNR1GT pUltraBR MjY dtyrS tyrTV::inact tyrU::inact lambda::tolCDepositorBacterial ResistanceSpectinomycinSpeciesEscherichia coliAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG.Myc-SARS2_3CL
Plasmid#200120PurposeExpresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL in mammalian cellsDepositorInsertSARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)
UseTagsMycExpressionMammalianMutationPromoterCAGAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIFU002
Plasmid#174299PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the N501Y mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombinationDepositorInsertSpike RBD N343Q, N501Y (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHA and cMycExpressionYeastMutationN343Q, N501YPromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIFU001
Plasmid#174298PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombinationDepositorInsertSpike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
UseTagsHA and cMycExpressionYeastMutationN343Q, E484KPromoterAvailable sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEnt L1L3 EF1a-tTA-3
Plasmid#24415DepositorTypeEmpty backboneUseEntry vectorTagsExpressionMutationPromoterAvailable sinceJune 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 GW delCMV
Plasmid#29496DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEnt R3L2-MCS
Plasmid#26799DepositorInsertmultiple cloning sequence
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEnt L1L3-MCS
Plasmid#26798DepositorInsertmultiple cloning sequence
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceMay 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTFG005_Spike_Inducible_3xFlag
Plasmid#191578PurposeInducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).DepositorInsertSARS-CoV-2 Spike Protein (S Synthetic)
UseLentiviralTags3x FLAGExpressionMammalianMutationPromoterTRE3GAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTFG011_Spike_Parental_D614G_3xFlag
Plasmid#191571PurposeSARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.DepositorInsertSARS-CoV-2 Spike Protein (S Synthetic)
UseTags3x FLAGExpressionMammalianMutationD614G mutation. Last 19 amino acids truncated.PromoterCMVAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag
Plasmid#191572PurposeMutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.DepositorInsertSARS-CoV-2 Spike Protein (S Synthetic)
UseTags3x FLAGExpressionMammalianMutationD614G and F486S (confers reduced binding affinity…PromoterCMVAvailable sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO DNAJA1
Plasmid#19464DepositorInsertDNAJA1 (DNAJA1 Human)
UseTagsExpressionMammalianMutationD244GPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFM038
Plasmid#40141DepositorInsertpar-2 RNAi resistant (R183-5A + 7SA) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationR183-5A + 7SA; base pairs 6-523 of par-2 were re…Promoterpie-1Available sinceOct. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFM041
Plasmid#40144DepositorInsertpar-2 RNAi resistant (S241A) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationS241A; base pairs 6-523 of par-2 were recoded to…Promoterpie-1Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFM039
Plasmid#40142DepositorInsertpar-2 RNAi resistant (R183-5A + S241A) (par-2 Nematode)
UseGateway destination vectorTagsGFPExpressionWormMutationR183-5A + S241A; base pairs 6-523 of par-2 were …Promoterpie-1Available sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits only
