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We narrowed to 24,534 results for: CRISPR

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Showing: 10841 - 10860 of 24534 results

  5. qTAG-N-Solo-mStayGold-CENPA

    Plasmid
    #227283
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the CENPA locus. For centromere visualization. To be co-transfected with sgRNA plasmid px330-CENPA (Addgene #227282)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    CENPA Homology Arms flanking a mStayGold Tag (CENPA Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227283-plasmid-map-sequence-id-451297

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  9. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid
    #183409
    Purpose
    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183409-plasmid-map-sequence-id-361258

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  10. pLCKO

    Plasmid
    #73311
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.
    Depositor
    Jason Moffat
    Article
    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73311-plasmid-map-sequence-id-134941

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  13. pLSB-NAT

    Plasmid
    #166698
    Purpose
    Combined sgRNA/Cas9 pLSB vector with natMX6 (cloNAT) marker. Golden Gate-ready for cloning custom sgRNA sequences.
    Depositor
    Robin Allshire
    Article
    Torres-Garcia et al Wellcome Open Res. 2020 Nov 24;5:274. do
    Inserts
    tRNA promoter : sgRNA cassette
    adh15 promoter : Cas9 codon-optimised for S. pombe
    Expression
    Yeast
    Promoter
    adh15 promoter and tRNA promoter
    Available Since
    April 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    166698-plasmid-map-sequence-id-329407

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  14. SunTagng22aa

    Plasmid
    #106438
    Purpose
    Encodes a SunTag CRISPR cas9 system that does not contain a guide RNA and therefore, does not target the TET1 catalytic domain to any specific region of the genome.
    Depositor
    Steven Jacobsen
    Article
    Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11
    Insert
    NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106438-plasmid-map-sequence-id-309504

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  15. qTAG-N-Solo-mStayGold-RAB7A

    Plasmid
    #227298
    Purpose
    Donor template for mStayGold insertion into the N-terminus of the RAB7A locus. For endosome visualization. To be co-transfected with sgRNA plasmid px330-RAB7A (Addgene #227297)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    RAB7A Homology Arms flanking a mStayGold Tag (RAB7A Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227298-plasmid-map-sequence-id-451536

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  16. pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin

    Plasmid
    #187956
    Purpose
    FKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    FKBP12_F36V-hHDAC4-SpdCas9-tagBFP
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available Since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187956-plasmid-map-sequence-id-373523

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  19. pRC49-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-2A-EGFP_NLS-WPRE

    Plasmid
    #123363
    Purpose
    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat and U6 terminator, with constitutive expression of puromycin resistance, Firefly luciferase, and nuclear EGFP.
    Depositor
    Sidi Chen
    Article
    Chow et al Nat Methods. 2019 May;16(5):405-408. doi
    Insert
    EGFP-NLS
    Use
    CRISPR, Lentiviral, and Luciferase
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    123363-plasmid-map-sequence-id-240469

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  20. qTAG-C-mStayGold-Puro-H2BC11-MMEJ

    Plasmid
    #227333
    Purpose
    MMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227333-plasmid-map-sequence-id-452137

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Showing: 10841 - 10860 of 24534 results