We narrowed to 4,708 results for: Bre;

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eGFP L202 gRNA

Plasmid

#119132

Purpose

gRNA that guides Cas9 and APOBEC complexes to L202 in eGFP.

Depositor

Reuben Harris

Article

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Insert

eGFP L202 gRNA

Use

CRISPR

Mutation

None

Promoter

U6

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pRG646_lexO-HO_LexA-TF_LEU2MX

Plasmid

#154815

Purpose

Inducible expression of HO endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Article

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

HO endonuclease (HO Budding Yeast)
LexA-ER-B112

Tags

FLAG

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a SnoopTag-mEGFP-SpyTag

Plasmid

#72325

Purpose

Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

Depositor

Mark Howarth

Article

Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

Insert

pET28a SnoopTag-mEGFP-SpyTag

Tags

N-terminal His6 tag

Expression

Bacterial

Available Since

Feb. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pSW002-PpsbA

Plasmid

#108236

Purpose

Broad host bacterial expression vector with constitutive PsbA promoter

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

PsbA promoter

Expression

Bacterial

Available Since

May 7, 2018

Availability

Academic Institutions and Nonprofits only

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p3868 HA-E6-AP

Plasmid

#8648

Depositor

Peter Howley

Article

Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

Insert

E6-AP (UBE3A Human)

Tags

HA

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-BCL2-hygro

Plasmid

#222581

Purpose

PiggyBac transposon plasmid for constitutive expression of BCL2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

BCL2 (BCL2 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-HaloTag-CNA35

Plasmid

#215457

Purpose

Encodes the bacterial collagen-binding protein CNA35 with N-terminal HaloTag.

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

CNA35

Tags

HaloTag

Expression

Bacterial

Mutation

Includes stop codon

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti6.3/TO/V5-DEST-EGFP

Plasmid

#215538

Purpose

Lentiviral transfer vector to express the EGFP fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

EGFP

Use

Lentiviral

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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mOPN-PB0

Plasmid

#106449

Purpose

Bacterial expression of murine osteopontin

Depositor

Georg Weber

Article

Zhang et al Mol Cell Biol. 2003 Sep . 23(18):6507-19

Insert

osteopontin (Spp1 Mouse)

Use

Retroviral

Mutation

WT

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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BFP Homology Donor

Plasmid

#149372

Purpose

Promoter-less BFP cDNA with two homology arms to the lenti-CDDR reporter for HDR detection

Depositor

Tarek Abbas

Article

Eki et al Nucleic Acids Res. 2020 Oct 17. pii: 592

Insert

CDDR BFP Homology Donor

Expression

Mammalian

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-TET1

Plasmid

#222572

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of TET1

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

TET1 (TET1 Human)

Expression

Mammalian

Available Since

Aug. 15, 2024

Availability

Academic Institutions and Nonprofits only

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human MTAP

Plasmid

#64077

Purpose

Expression of human 5'-methylthioadenosine phosphorylase

Depositor

Vern Schramm

Article

Guan et al Biochemistry. 2011 Nov 29;50(47):10408-1

Insert

5'-methylthioadenosine phosphorylase (MTAP Synthetic, Human)

Tags

Histidine tag and TEV

Expression

Bacterial

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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Hsp68-LacZ-Gateway

Plasmid

#37843

Depositor

Nadav Ahituv

Article

Pennacchio et al Nature. 2006 Nov 23;444(7118):499-502. E

Type

Empty backbone

Use

Mouse Targeting

Promoter

Mouse Hsp68 minimal promoter

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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p4054 HA-E6AP isoform II

Plasmid

#8658

Depositor

Peter Howley

Article

Kao et al J Virol 2000 Jul;74(14):6408-17.

Insert

E6-AP isoform II (UBE3A Human)

Tags

HA

Expression

Mammalian

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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gRNA_SRR124.5_tagBFP

Plasmid

#163753

Purpose

gRNA expression vector containing the tagBFP fluorescent marker to target the region upstream of the SRR124 SOX2 enhancer in human cells.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

gRNA_SRR124.5

Use

CRISPR

Tags

tagBFP

Expression

Mammalian

Promoter

U6

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMM119

Plasmid

#127213

Purpose

BeYDV replicon with constitutive GFP expression, WUS2, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

GFP, WUS2, sgRNA

Expression

Plant

Mutation

codon optimized CDSs

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro GFP siRNA

Plasmid

#12273

Depositor

Bob Weinberg

Article

Orimo et al Cell. 2005 May 6. 121(3):335-48.

Insert

GFP siRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only

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pGSKU

Plasmid

#72243

Purpose

Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
kanMX4
I-SceI

Expression

Bacterial

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pSL4393 (pClpX_Eco)

Plasmid

#200907

Purpose

Expresses human codon-optimized E. coli ClpX from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

EcoClpX

Tags

NLS

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLifeAct-miRFP703

Plasmid

#79993

Purpose

F-Actin labeling with near-infrared fluorescent protein miRFP703

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

Lifeact

Tags

miRFP703

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pLPCX-PECAMTS

Plasmid

#45850

Depositor

Martin Schwartz

Article

Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

Insert

PECAM1 (PECAM1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pSL3713 (pTnsC_Pse)

Plasmid

#200900

Purpose

Expresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseTnsC

Tags

3xNLS

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-YTHDC2

Plasmid

#222558

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of YTHDC2

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

YTHDC2 (YTHDC2 Human)

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSW002-Pc-E2-Crimson

Plasmid

#111252

Purpose

Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with E2-Crimson

Depositor

Rosemarie Wilton

Article

Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

Insert

E2-Crimson

Expression

Bacterial

Promoter

Pc

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-KLF5

Plasmid

#40900

Depositor

Jin-Tang Dong

Article

Chen et al Oncogene. 2002 Sep 26;21(43):6567-72.

Insert

Kruppel-like factor 5 (KLF5 Human)

Expression

Mammalian

Promoter

PCMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag-Smad4M

Plasmid

#14959

Depositor

Joan Massague

Article

Kang et al Proc Natl Acad Sci U S A. 2005 Sep 27. 1

Insert

Smad4 (SMAD4 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pPB-cT3G-cERP2-DDX4

Plasmid

#222527

Purpose

PiggyBac transposon plasmid for doxycycline inducible expression of DDX4

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

DDX4 (DDX4 Human)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA 3.1 Flag-rat-PTP1B

Plasmid

#35988

Depositor

Anna Huttenlocher

Article

Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

Insert

protein tyrosine phosphatase 1B (Ptpn1 Rat)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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pCVL.SFFV.Kozak.HA.NLS.SceOpt.IRES.BFP

Plasmid

#45574

Purpose

Expresses I-Sce I nuclease in mammalian cells with BFP tracker in lentiviral backbone

Depositor

Andrew Scharenberg

Article

Kuhar et al Nucleic Acids Res. 2013 Oct 10.

Insert

co I-Sce I

Use

Lentiviral

Tags

HA, IRES BFP, and NLS

Expression

Mammalian

Mutation

co indicates codon optimization for mammalian exp…

Promoter

SFFV

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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p4055 HA-E6AP isoform III

Plasmid

#8659

Depositor

Peter Howley

Article

Kao et al J Virol 2000 Jul;74(14):6408-17.

Insert

E6-AP isoform III (UBE3A Human)

Tags

HA

Expression

Mammalian

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only