We narrowed to 34,877 results for: CaS

1. pCLV3Cas-LT0

   Plasmid

   #210548

   Purpose

   Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT0 reporter

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT0::mCherrySSM
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGGB-SpCas9

   Plasmid

   #210533

   Purpose

   Cloning A.thaliana codon optimized SpCas9 CDS as GreenGate module B

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   SpCas9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-T3-hCasD10A-pA

   Plasmid

   #51638

   Purpose

   Expresses Cas9-nickase in mammalian cells and zygotes.

   Depositor

   Wataru Fujii

   Article

   Fujii et al Biochem Biophys Res Commun. 2014 Jan 31.

   Insert

   Codon-optimized Cas9 D10A
   

   Use

   CRISPR

   Tags

   FLAG, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG promoter

   Available since

   March 14, 2014

   Availability

   Academic Institutions and Nonprofits only

4. SpCas9 iFE

   Plasmid

   #190138

   Purpose

   Expresses SpCas9-POLB (iFE)

   Depositor

   Timothy Lu

   Article

   Nakade et al bioRxiv 2022.12.05.518807

   Insert

   SpCas9-POLB (HiFi iFE)
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Codon Optimized for mammalian cells

   Promoter

   CBh

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSAVED-CHAT_PCaspase_PCi_PC-σ

   Plasmid

   #214040

   Purpose

   Bacterial expression plasmid for SAVED-CHAT, PCaspase, PCi, and PC-σ from Haliangium ochraceum

   Depositor

   Raymond Staals

   Article

   Steens et al Science. 2024 Feb 2;383(6682):512-519. d

   Insert

   SAVED-CHAT-Pcaspase-Pci-PC-σ
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lacUV5 promoter

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9(BB)-2A-Neo_h53BP1_gRNA_D

   Plasmid

   #110300

   Purpose

   Expresssion of Cas9-T2A-neomycin resistant gene and a gRNA targeting exon 10 of human 53BP1

   Depositor

   Chris Kok-Lung Chan

   Article

   Addis Jones et al Nat Commun. 2019 Jun 28;10(1):2861. doi:

   Insert

   p53-binding protein 1 (TP53BP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAct:dCas9-VP64

   Plasmid

   #78901

   Purpose

   Expresses dCas9-VP64 under actin promoter for SAM CRISPRa in Drosophila cells

   Depositor

   Norbert Perrimon

   Article

   Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

   Insert

   dCas9-VP64
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   pActin (Drosophila)

   Available since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pF(UG) hSyn spCas9

   Plasmid

   #179725

   Purpose

   human synapsin (hSyn)-driven spCas9 lentiviral vector for CRISPR/HITI

   Depositor

   Edwin Chapman

   Article

   Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

   Insert

   spCas9
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRhaQCas-BsaI

   Plasmid

   #170631

   Purpose

   Expression of CRISPR-associated transposases

   Depositor

   Sheng Yang

   Article

   Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

   Insert

   VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(BsaI)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJWV102-wtcas9sp

    Plasmid

    #99612

    Purpose

    Plasmid containing wild-type cas9sp

    Depositor

    Morten Kjos, Jan-Willem Veening

    Article

    van Raaphorst et al Proc Natl Acad Sci U S A. 2017 Jul 3. pi

    Insert

    cas9sp
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PczcD

    Available since

    Sept. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-T3-SpCAS9-NG-pA

    Plasmid

    #131465

    Purpose

    Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

    Insert

    Codon optimized SpCas9-NG
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. dSaCas9-KRAB

    Plasmid

    #205250

    Purpose

    In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.

    Depositor

    Rasmus Bak

    Insert

    dSaCas9-KRAB
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-Caspase 12

    Plasmid

    #35574

    Depositor

    Junying Yuan

    Article

    Nakagawa et al Nature. 2000 Jan 6. 403(6765):98-103.

    Insert

    Caspase 12 (Casp12 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 6, 2012

    Availability

    Academic Institutions and Nonprofits only

14. 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO

    Plasmid

    #172225

    Purpose

    Cas9-2A-GFP expression vector bearing two sgRNAs targeting the centromeric human SOX17 CTCF-boundary.

    Depositor

    Alexander Meissner

    Article

    Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

    Insert

    sgRNA
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYPQ-NF-L1-dMb2Cas12a

    Plasmid

    #177683

    Purpose

    Gateway entry clone (attL1 & attR5) for dimeric FokI-dMb2Cas12a genome editing

    Depositor

    Yiping Qi

    Article

    Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

    Insert

    FokI-dMb2Cas12a
    

    Use

    CRISPR; Gateway compatible foki-dmb2cas12a entry …

    Tags

    NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX165-evoCas9

    Plasmid

    #140569

    Purpose

    Expresses evoCas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    M495V Y515N K526E R661Q

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. UAST-LbCas12a

    Plasmid

    #140621

    Purpose

    Gal4 dependent expression of LbCas12a in Drosophila

    Depositor

    Michael Boutros

    Article

    Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

    Insert

    LbCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAST

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pXFokI-dCas9

    Plasmid

    #60901

    Purpose

    Dual Expression Vector for FokI-dCas9 and gRNA

    Depositor

    Bruce Conklin

    Article

    Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_Anc689-nCas9

    Plasmid

    #163526

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    Anc689-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLBH546_Tet-Cas14b1Locus

    Plasmid

    #112503

    Purpose

    Expresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

    Depositor

    Jennifer Doudna

    Article

    Harrington et al Science. 2018 Oct 18. pii: science.aav42

    Insert

    Cas14b1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Oct. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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