We narrowed to 92,654 results for: lias

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(559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

Plasmid

#163024

Purpose

Expression of CRISPRi with U6-gRNA (Bsa1 sites)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

AAV

Tags

KRAB, SV40 polyA, and myc NLS

Expression

Mammalian

Promoter

hEF-1a

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-METTL3

Plasmid

#53739

Purpose

For expression of METTL3 (methyltransferase-like 3) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Methyltransferase-like 3 (METTL3 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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ATF4 3: Mouse ATF4 (CHOP11/cATF), 5'UTR and AUG-luc

Plasmid

#21850

Depositor

David Ron

Article

Harding et al Mol Cell. 2000 Nov . 6(5):1099-108.

Insert

ATF4 (Atf4 Mouse)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

ATF4 5' UTR and AUG fused to Luciferase driv…

Available Since

Oct. 8, 2009

Availability

Academic Institutions and Nonprofits only

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PiggyBac EF1a-Tet-on 3G-Puro U6 BbsI

Plasmid

#159756

Purpose

PiggybacTransposon-based expression control of Tet-on 3G, Puro and Cas13d U6 gRNA backbone

Depositor

Xiaoping Bao

Article

Chang et al Biomaterials. 2022 May 6;285:121569. doi

Type

Empty backbone

Use

Piggybac transposon

Expression

Mammalian

Promoter

U6

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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eGFPC1 FKBP

Plasmid

#67529

Purpose

Expresses GFP fused FKBP

Depositor

Stephen Tait

Article

Ichim et al Mol Cell. 2015 Mar 5;57(5):860-72. doi:

Insert

FKBP

Tags

eGFP

Expression

Mammalian

Mutation

F36V

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pBabe puro HA PIK3CA H1047R

Plasmid

#12524

Purpose

Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

H1047R. Tumor-derived allele.

Available Since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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a1Ha-pcDNA3

Plasmid

#45809

Depositor

Edward Perez-Reyes

Article

Cribbs et al Circ Res. 1998 Jul 13;83(1):103-9.

Insert

a1Ha (CACNA1H Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2013

Availability

Academic Institutions and Nonprofits only

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pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro

Plasmid

#205631

Purpose

Expression of TnpBmax in mammalian cells

Depositor

Gerald Schwank

Article

Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

Insert

ISDra2-TnpB optimized for mammalian expression

Tags

NLS-3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pSH-EFIRES-P-AtAFB2-mCherry

Plasmid

#129716

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

mCherry

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5c-Myc

Plasmid

#186616

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform C fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5c (KIF5C Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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CIBN-CAAX

Plasmid

#79574

Purpose

expression of N-terminal portion of CIB1 with a C-terminal CAAX box from KRras for plasma-membrane targeting

Depositor

Pietro De Camilli, Olof Idevall-Hagren

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

CIBN (CIB1 Mustard Weed)

Tags

C-terminal CAAX-box

Expression

Mammalian

Mutation

K93A, R94A, K106A, K107A (please see depositor co…

Promoter

CMV

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pHR-CMV_TetR-HA-NLS-P2A-BSD-Myc

Plasmid

#113899

Purpose

Mammalian constitutive expression of HA-tagged Tetracyclin repressor protein and Myc-tagged blasticidin deaminase

Depositor

A. Radu Aricescu

Article

Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

Insert

TetR-HA-NLS-P2A-BSD-Myc

Use

Lentiviral

Tags

HA-Myc

Promoter

CMV-MIE

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin- soCoChR-GFP

Plasmid

#107708

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Has Service

AAV9

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Promoter

hSynapsin

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hSTING (S366A)

Plasmid

#214155

Purpose

Transient expression of STING

Depositor

Nan Yan

Article

Wu et al Immunity. 2020 Jul 14;53(1):115-126.e5.

Insert

STING (S366A) (STING1 Human)

Expression

Mammalian

Mutation

S366A

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Target-ACEmax

Plasmid

#139105

Purpose

Mammalian Target-ACEmax expression vector (pSI-625)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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KRAB-dCas9

Plasmid

#112195

Purpose

KRAB fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Insert

KRAB (ZNF10 Human)

Use

CRISPR

Tags

3XFLag-NLS-KRAB-dCas9-NLS

Expression

Mammalian

Promoter

CMV

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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Braf

Plasmid

#40775

Depositor

Dustin Maly

Article

Rose et al Nat Chem Biol. 2016 Nov 21. doi: 10.1038

Insert

Braf (BRAF Human)

Tags

FLAG

Expression

Mammalian

Mutation

Starting Met removed

Promoter

CMV

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-dCas9-BFP

Plasmid

#46910

Purpose

Human expression vector containing SFFV promoter, dCas9 that is fused to 2x NLS and tagBFP

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

dCas9-BFP fusion

Use

CRISPR and Lentiviral

Tags

2xNLS, BFP, and HA

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC7

Plasmid

#231656

Purpose

Expresses human ZDHHC7 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC7 (ZDHHC7 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC3

Plasmid

#231652

Purpose

Expresses human ZDHHC3 protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

DHHC3 (ZDHHC3 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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