We narrowed to 23,148 results for: REP
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Plasmid#110191PurposeExpression of mouse MeCP2 (deltaNIC) in mammalian cellsDepositorInsertMeCP2_e2 delta NIC (mouse cDNA) - EGFP (Mecp2 Mouse)
TagsEGFPExpressionMammalianMutationdelta N and C termini. Intervening region replace…PromoterCMVAvailable SinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
2XMyc-LRRK2-deltaHeat
Plasmid#25068DepositorInsertLRRK2 (LRRK2 Human)
Tags2XMycExpressionMammalianMutationContains the LRR-ROC-COR-Kinase-WD40 domains; Del…Available SinceNov. 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEA01
Plasmid#170766PurposeUtilisation of pSAM2 excision tool in Amycolatopsis sppDepositorInsertsxis, int
oriT
pac
ExpressionBacterialPromotertrcpAvailable SinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-FlaviA-GFP-puro
Plasmid#140088PurposeFluorescence activatable reporter of flavivirus NS2B-NS3 protease activityDepositorInsertFlaviA-GFP
UseLentiviral; Low expressionTags6XHis tag and GFPExpressionMammalianPromoterCMVAvailable SinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q139
Plasmid#111738PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ139 (polyQ repeat)PromoterCMV and P10Available SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBacMam2-DiEx-LIC-N-flag_huntingtin_full-length_Q51
Plasmid#111734PurposeBaculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cellsDepositorInsertHTT (HTT Human)
UseBaculovirus expressionTagsFLAGExpressionMammalianMutationQ51 (polyQ repeat)PromoterCMV and P10Available SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBV-Luc/Frag-F
Plasmid#14053DepositorInsertc-myc promoter (-109/-3) (MYC Human)
UseLuciferaseExpressionMammalianMutation-109/-3 (relative to the P2 transcription initiat…Available SinceMay 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
Membrane mNeonGreen
Plasmid#198057PurposePlasma membrane localizing mNeonGreen using Human LCK proto-oncogene sequenceDepositorInsertLCK membrane mNeonGreen (LCK Human, A.victoria)
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmNeonGreenMutationTandem repeat of N-terminal LCK membrane targetin…Promotersp6Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)
Plasmid#128164PurposeUV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).DepositorInsert10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
UseSynthetic BiologyTagsFLAG and zif268ExpressionBacterialPromoterUV5Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA2-Cul4A-deltaROC
Plasmid#20713DepositorInsertCullin 4A (CUL4A Human)
TagsHA2ExpressionMammalianMutationDeletion of residues 594 to 612 from N-terminusAvailable SinceMay 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pOSV811
Plasmid#126604Purposemodular integrative vector for the assembly of DNA fragments and expression in Streptomyces speciesDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Membrane EGFP
Plasmid#198056PurposePlasma membrane localizing EGFP using Human LCK proto-oncogene sequenceDepositorInsertLCK membrane EGFP (LCK Synthetic, Human, A. victoria)
UseSea urchin, mammal, zebrafishTagsEGFPMutationTandem repeat of N-terminal LCK membrane targetin…Promotersp6Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β3 Tubulin
Plasmid#170552PurposeEncodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.DepositorTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectAvailable SinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Human a3 nicotinic receptor subunit EM construct
Plasmid#163960PurposepEZT-BM vector with alpha3 gene after CMV promoter, published EM constructDepositorInsertHuman alpha3 nicotinic acetylcholine receptor subunit EM construct
UseBaculovirus (bacmam) production and mammalian exp…Tagsapocytochrome b(562)RIL (BRIL)ExpressionMammalianMutationAsn348-Ser402 replaced with apocytochrome b(562)R…PromoterCMVAvailable SinceFeb. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHC01
Plasmid#106900PurposeRatiometric gas biosensor plasmid for 3-oxo-C12-HSL sensingDepositorInsertsLasR
Methyl Halide Transferase
Ethylene Forming Enzyme
Red Fluorescent Protein
ExpressionBacterialAvailable SinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTriEX-Antenna-GDI Rac1
Plasmid#83359PurposeExpression of Antenna-Rac1DepositorInsertRac1 (RAC1 Human)
TagsFRET Antenna (mCerulean-cpVenus) and HisExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OK+9MS
Plasmid#80482PurposepiggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)DepositorUsePiggybac transposonExpressionMammalianMutationKlf4 [1-483]PromotertetOAvailable SinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMXs-ms-c-Myc-L420P
Plasmid#50776Purposeretroviral expression of mouse c-Myc L420P mutantDepositorUseRetroviralExpressionMammalianMutationL420P (does not bind to Max)Available SinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
nes714tk/lacZ
Plasmid#47614Purposeused to create transgenic mice expressing LacZ reporter with Nestin enhancerDepositorInsertnestin 2nd intron fragment (NES Human)
UseEnhancer reporterTagsHSV tk promoter and lacZMutation714 bp from 3' end of 2nd intronPromoterHSV tk promoterAvailable SinceAug. 28, 2013AvailabilityAcademic Institutions and Nonprofits only