We narrowed to 25,691 results for: invs

1. KHBD00649

   Plasmid

   #39711

   Depositor

   Bart Deplancke

   Article

   Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

   Insert

   CG6172 (vnd Fly)
   

   Use

   Gateway donr vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3-FLAG-HA-CAD S1900A

   Plasmid

   #46240

   Purpose

   FLAG-HA-CAD with S1900 mutated to alanine

   Depositor

   Brendan Manning

   Article

   Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

   Insert

   carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Serine 1900 changed to Alanine (S1900A)

   Promoter

   CMV

   Available Since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

3. Kv7.4 (1-645, Δ368-523)/HA tag/pcDNA3.1

   Plasmid

   #111459

   Purpose

   Electrophysiology in HEK cell

   Depositor

   Dan Minor

   Article

   Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

   Insert

   potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
   

   Use

   Tags

   Ha Tag

   Expression

   Mammalian

   Mutation

   Kv7.4 (1-645, Δ368-523), C643A

   Promoter

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRC/RSV-rat Agt

   Plasmid

   #122104

   Purpose

   Expression of angiotensinogen

   Depositor

   John Chan

   Article

   Zhang et al J Am Soc Nephrol. 2002 Feb;13(2):302-12.

   Insert

   angiotensinogen (Agt Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   RSV promoter

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCR8/GW/TOPO-ELK1

   Plasmid

   #98616

   Purpose

   Gateway entry TOPO TA cloning vector for ELK1

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   ELK1 (Elk1 Mouse)
   

   Use

   Topo ta cloning vector

   Tags

   Expression

   Mutation

   Q230R

   Promoter

   Available Since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA3.1(+)-RIα(D/D+linker)-EGFP

   Plasmid

   #181842

   Purpose

   EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-(D/D+linker)EGFP (PRKAR1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Contains only residues 12-112 of PKA RIα.

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3-HtrA2-FLAG S142D

   Plasmid

   #15939

   Depositor

   L. Miguel Martins

   Article

   Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

   Insert

   HtrA2 (HTRA2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S142D

   Promoter

   Available Since

   Nov. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pDEST27-GST-ATXN1 FL(30Q)-S776D

   Plasmid

   #21755

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 32 amino acids (30 Q…

   Promoter

   CMV

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pAc5.1B-EGFP-DmThor-V5His6

   Plasmid

   #79408

   Purpose

   Expresses EGFP-tagged and C-terminal V5His6-tagged DmThor in S2 cells

   Depositor

   Elisa Izaurralde

   Article

   Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

   Insert

   DmThor (Thor Fly)
   

   Use

   Schneider 2 cell expression

   Tags

   EGFP and V5-His6

   Expression

   Mutation

   Promoter

   Ac5 promoter

   Available Since

   July 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Opto-GPR19

    Plasmid

    #106007

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR19; Opto-GPR19; A9)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR19 (GPR19 Bovine, Human)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcdna3 Flag Jip3b(T266A, T276A, T287A)

    Plasmid

    #58924

    Purpose

    interacts with MAPK signaling module

    Depositor

    Roger Davis

    Article

    Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

    Insert

    mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    changed T266A, T276A, T287A

    Promoter

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pTBL681 CHYRON4 integration construct

    Plasmid

    #126449

    Purpose

    To integrate the CHYRON4 locus at AAVS1 in human cells.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6/3xLacO-CHYRON4 hgRNA
    pCMV-puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    CMV and human U6/3xLacO

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. KA801: pMVP (L3-L2) HA tag + pA; CMV::eGFP-P2A-TETa-pA

    Plasmid

    #121800

    Purpose

    pMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven GFP-P2A-TETa

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    HA epitope tag-polyA + CMV::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

    Plasmid

    #121819

    Purpose

    pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA5FRT/Flag-hINO80N1 (1-262)

    Plasmid

    #29441

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

    Insert

    INO80 (INO80 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    INO80 1-262 (a.a.)

    Promoter

    Available Since

    Aug. 1, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3-FLAG-hDIXDC1 S592A

    Plasmid

    #61223

    Purpose

    Mammalian expression vector encoding long isoform of human DIXDC1

    Depositor

    Reuben Shaw

    Article

    Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

    Insert

    human DIXDC1 (DIXDC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed Serine 592 to Alanine

    Promoter

    CMV

    Available Since

    Jan. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

17. Myc-Idax DBM

    Plasmid

    #68554

    Purpose

    This plasmid expresses full length IDAX containing a DNA binding mutation.

    Depositor

    Anjana Rao

    Article

    Ko et al Nature. 2013 May 2;497(7447):122-6. doi:

    Insert

    IDAX-DBM (Cxxc4 Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T162A, H164A and Q165A mutations

    Promoter

    EF1a

    Available Since

    Oct. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRD439

    Plasmid

    #168465

    Purpose

    For the insertion pf NLS-mTurquoise2-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    NLS-mTurquoise2-H-NSdbd-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEF5/FRT/DEST-3xHA/mGli2 P5-6D

    Plasmid

    #51254

    Purpose

    Encodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to Asp

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli2 (Gli2 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV

    Expression

    Mammalian

    Mutation

    amino acids 923,956 mutated to Asp

    Promoter

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

20. p4948 pcDNA4c His Xpress SV40NLS hBrd4 CTD

    Plasmid

    #14442

    Depositor

    Peter Howley

    Article

    You et al Cell. 2004 Apr 30. 117(3):349-60.

    Insert

    Brd4 CTD (BRD4 Human)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    amino acids 1047-1362 of Brd4 (the CTD). Function…

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only