We narrowed to 15,234 results for: invs

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pDEST53-LRRK2-K1347A

Plasmid

#25052

Depositor

Mark Cookson

Article

Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

Insert

LRRK2 (LRRK2 Human)

Tags

GFP

Expression

Mammalian

Mutation

Lysine 1347 mutated to Alanine

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pENTR-2B-Gata4

Plasmid

#98615

Purpose

Gateway entry vector for Gata4

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

Gata4 (Gata4 Mouse)

Use

Entry vector

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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KCNE2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176980

Purpose

Mammalian expression vector encoding KCNE2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

KCNE2 (KCNE2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Gephyrin GC

Plasmid

#68818

Purpose

Flag tagged mammalian expression of GC domain

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

3xFLAG

Expression

Mammalian

Mutation

NIL

Promoter

CMV

Available Since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Dakap-Venus-cpVenus-FLARE-AKAR

Plasmid

#123338

Purpose

Mitochondria-targeted yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

DAKAP-Venus-cpVenus-FLARE-AKAR

Tags

N-terminal 30 amino acids from DAKAP1, Venus, and…

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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Opto-GPR18

Plasmid

#106006

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR18; Opto-GPR18; A8)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR18 (GPR18 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

Plasmid

#113953

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

delete N-terminal 21 residues, I132S, S212N, and …

Promoter

cmv

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ Flag-RAGB99L

Plasmid

#128101

Purpose

Expresses constitutively GTP-bound RAGB in mammalian cells in a G418-selectable plasmid

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

Ras related GTP binding B (RRAGB Human)

Tags

Flag

Expression

Mammalian

Mutation

Q99L (CAA to CTA)

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pKK-NoTag

Plasmid

#105767

Purpose

Control vector for pKK series (encodes only SLIC arms (TEV-L and TEV-R)); expression without tag. Useful to design other vectors; any tag can be added.

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Expression

Mammalian

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-hMSN

Plasmid

#211823

Purpose

Express human MSN in mammalian cells

Depositor

Haian Fu

Article

Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

Insert

MSN (MSN Human)

Tags

GST tag

Expression

Mammalian

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

Plasmid

#121814

Purpose

pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_DIO_R106W-HaloTag

Plasmid

#164063

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R106W mutation

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 R106W (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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gRNA-hIRF-1 #12

Plasmid

#61079

Purpose

Expresses a guide RNA (gRNA) to target human IRF-1 promoter

Depositor

Hodaka Fujii

Article

Fujita et al Biochem Biophys Res Commun. 2013 Sep 13;

Insert

gRNA_hIRF1 promoter #12 (IRF1 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-nAChr-Alpha6

Plasmid

#48812

Purpose

this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

Depositor

Henry Lester

Article

Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

Insert

nAChr-alpha6 (Chrna6 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Flag RdgBbeta (long form) splice variant 1

Plasmid

#58275

Purpose

mammalian expression of human RdgBbeta splice variant 1

Depositor

Shamshad Cockcroft

Article

Garner et al Biochem J. 2011 Oct 1;439(1):97-111. doi

Insert

RdgBbeta (PITPNC1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
HA-MBP-TEVpCS-ADGRG5 CTF-FLAG

Plasmid

#217703

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRG5 (ADGRG5 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-226

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HtrA2-FLAG S400D

Plasmid

#15940

Depositor

L. Miguel Martins

Article

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S400D

Available Since

Nov. 5, 2007

Availability

Academic Institutions and Nonprofits only
HA-MBP-TEVpCS-ADGRB2 CTF-FLAG

Plasmid

#217682

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRB2 (BAI2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-911

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Opto-GPR27

Plasmid

#106013

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR27; Opto-GPR27; B3)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR27 (GPR27 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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(367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)

Plasmid

#168099

Purpose

HA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA

Depositor

Christina Woo

Article

Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

Insert

HA-nUbc-OGA(544-706)

Tags

HA-tag

Expression

Mammalian

Mutation

OGA truncated to contain residues 544-706

Promoter

T7/CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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