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SEPLuc-IRES-Antares

Plasmid

#141085

Purpose

precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

SEPLuc-EMCV IRES-Antares

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pDRF’- AmTryoshka1;3-GS

Plasmid

#100967

Purpose

Yeast expression of control vector for fluorescent sensors reporting the activity of ammonium transceptors.

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

AmTryoshka1;3-GS (AMT1;3 Mustard Weed)

Expression

Yeast

Promoter

PMA

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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Mut.Blimp1pGL3 (Mutant human Blimp1 promoter in pGL3-basic)

Plasmid

#40341

Depositor

Alexander Dent

Article

Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Insert

Blimp1 promoter (mutated) (PRDM1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Two AP-1 sites at -1813 and -1647 replaced with B…

Promoter

Blimp1

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only
pEM01

Plasmid

#135414

Purpose

Expression of Hygromycin resistance from CMV promoter

Depositor

Nicolas Buchler

Article

Medina et al Elife. 2020 May 11;9. pii: 52741. doi: 1

Insert

CMVpr hph ADH1t

Expression

Bacterial and Yeast

Promoter

CMVpr

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL11057

Plasmid

#68253

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in barley

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promote:TaU6+sgRNA HvPM19_1

Use

Synthetic Biology

Expression

Plant

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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PX458_MBD1_iso1_2

Plasmid

#86304

Purpose

Encodes gRNA for 3' target of human MBD1_iso1

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MBD1_iso1 (MBD1 Human)

Use

CRISPR

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST myc MOV10 D645N

Plasmid

#51720

Purpose

plasmid expresses myc-tagged MOV10 D645N

Depositor

Markus Landthaler

Article

Gregersen et al Mol Cell. 2014 May 22;54(4):573-85. doi:

Insert

MOV10 D645N (MOV10 Human)

Tags

myc

Expression

Mammalian

Mutation

D645N

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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KHBD00486

Plasmid

#39591

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5965 (woc Fly)

Use

Gateway donr vector

Promoter

none

Available Since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
pW213-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR

Plasmid

#170811

Purpose

Lentiviral vector to co-express an sasgRNA with NLS-mNeonGreen

Depositor

Kenneth Yamada

Article

Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

NA

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_SHANK1-1/1

Plasmid

#91517

Purpose

Protein expression and purification of human SH3 domain construct SHANK1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SHANK1-1/1 (SHANK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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fgfprom luc

Plasmid

#69446

Purpose

basal plasmid containing minimal fgf4 promoter used for testing enhancer activity of DNAs inserted immediately upstream of the promoter.

Depositor

Lisa Dailey

Article

Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

Insert

minimal fgf4 promoter

Use

Luciferase

Expression

Mammalian

Available Since

May 5, 2016

Availability

Academic Institutions and Nonprofits only

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CMYA5/pACT2

Plasmid

#97208

Purpose

CMYA5 (aa 4039 – 4069) in a GAL4 AD vector, used for yeast-two hybrid

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Expression

Yeast

Available Since

Aug. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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CMV-CC2

Plasmid

#51221

Purpose

expression of amino acid 917-1040 of chicken DCTN1 p150Glued in mammalian cells

Depositor

Trina Schroer

Article

Quintyne et al J Cell Biol. 1999 Oct 18;147(2):321-34.

Insert

DCTN1 (DCTN1 Chicken)

Expression

Mammalian

Mutation

aa917-1040

Promoter

CMV

Available Since

March 20, 2014

Availability

Academic Institutions and Nonprofits only
pFA3

Plasmid

#46790

Purpose

To express ATPase inactive Fun30 in budding yeast

Depositor

Patrick Varga-Weisz

Article

Neves-Costa et al PLoS One. 2009 Dec 1;4(12):e8111. doi: 1

Insert

FUN30 (FUN30 Budding Yeast)

Tags

V5

Expression

Yeast

Mutation

ATP-binding site mutated replaced lysin 603 by ar…

Promoter

PGAL1

Available Since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only
PX458_GATAD2A_2

Plasmid

#86334

Purpose

Encodes gRNA for 3' target of human GATAD2A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against GATAD2A (GATAD2A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_GATAD2A_1

Plasmid

#86333

Purpose

Encodes gRNA for 3' target of human GATAD2A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against GATAD2A (GATAD2A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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CaM-RhoA(DP)-Venus

Plasmid

#31994

Depositor

Kevin Truong

Article

Mills et al Cell Calcium. 2010 Oct;48(4):195-201.

Insert

CaM-RhoA(DP) (RHOA Human)

Tags

YFP

Expression

Bacterial and Mammalian

Mutation

Q63L mutation in RhoA, E7S and D65Y in CaM

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00359

Plasmid

#34479

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7839 (CG7839 Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pPK1E-2+t

Plasmid

#48148

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_ITPKA

Plasmid

#65688

Purpose

Expresses ITPKA-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

inositol triphosphate 3-kinase A

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

contains residues 9-52

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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