168,004 results

1. pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

   Plasmid

   #169912

   Purpose

   Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

   Depositor

   Zhili Rong

   Article

   Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

   Insert

   dCjCas9
   

   Use

   CRISPR

   Tags

   10XGCN4, 2xSV40NLS, and SV40NLS-HA

   Expression

   Mammalian

   Mutation

   D8A, H559A in cjCas9

   Promoter

   CMV

   Available Since

   June 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEF-Bos TRIF Flag

   Plasmid

   #41550

   Depositor

   Tom Maniatis, Kate Fitzgerald

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   Trif (TICAM1 Human)
   

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

3. PKC epsilon DN

   Plasmid

   #21243

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC epsilon (Prkce Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K437R

   Available Since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

4. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

   Plasmid

   #213031

   Purpose

   Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration

   Depositor

   Martin S. Taylor, Donna Romero

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
   

   Use

   Luciferase

   Tags

   Firefly luciferase antisense intron (3' UTR)

   Expression

   Mammalian

   Promoter

   pTRE bidirectional tet-on

   Available Since

   July 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF.myc.ER-E2-Crimson

   Plasmid

   #38770

   Purpose

   In vivo visualization of the endoplasmic reticulum (can be used for colocalization studies)

   Depositor

   Benjamin Glick

   Article

   Strack et al Biochemistry. 2009 Sep 8;48(35):8279-81.

   Insert

   E2-Crimson
   

   Tags

   ER retention signal (KDEL), ER signal sequence (M…

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Aug. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tailless VinculinTS

   Plasmid

   #26020

   Depositor

   Martin Schwartz

   Article

   Grashoff et al Nature. 2010 Jul 8. 466(7303):263-6.

   Insert

   tailless vinculinTS
   

   Expression

   Mammalian

   Available Since

   Sept. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (AAV PHP.eB)

   Viral Prep

   #104495-PHPeB

   Purpose

   Ready-to-use AAV PHP.eB particles produced from pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (#104495). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE plasmid DNA. CAG-driven, Cre-dependent GCaMP7s calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

   Promoter

   CAG

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

8. pSH-EFIRES-P-AtAFB2-mCherry

   Plasmid

   #129716

   Purpose

   AAVS1 safe harbor targeting vector expressing AtAFB2-mCherry

   Depositor

   Elina Ikonen

   Article

   Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

   Insert

   AFB2 (AFB2 Mustard Weed)
   

   Use

   CRISPR and TALEN; Human safe harbor locus (a avs1…

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   EF1a

   Available Since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. MLRV

   Plasmid

   #118069

   Purpose

   Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Inserts

   TB:5xNF-κβ:RedF:bGHpA
   TB:4xTGF-β:FLuc:bGHpA
   TB:5xE-box:Renilla:bGHpA
   TB:2xp53:NLuc:bGHpA
   TB:6xAP-1:GrRenilla:bGHpA
   CMV:ELuc:bGHpA
   

   Use

   Luciferase and Synthetic Biology; Assembly of gb2…

   Expression

   Mammalian

   Promoter

   2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. ecAT3.10

    Plasmid

    #107215

    Purpose

    Mammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.

    Depositor

    Mathew Tantama

    Article

    Conley et al PLoS One. 2017 Nov 9;12(11):e0187481. do

    Insert

    ecAT3.10
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pnCas9-PBE

    Plasmid

    #98164

    Purpose

    Expresses CRISPR base editor in protoplasts

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-nCas9-UGI-NLS
    

    Expression

    Plant

    Promoter

    Ubi

    Available Since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmTurquoise2-Mito

    Plasmid

    #36208

    Purpose

    In vivo visualization of the mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    MTS (COX8A Human)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGIPz-mPD-L1

    Plasmid

    #121488

    Purpose

    Expression of mouse PD-L1 WT

    Depositor

    Mien-Chie Hung

    Article

    Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

    Insert

    PD-L1
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-TLR3-CFP

    Plasmid

    #13641

    Depositor

    Doug Golenbock

    Insert

    Toll-Like Receptor 3 (TLR3 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Available Since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQE31-rsEGFP2

    Plasmid

    #102879

    Purpose

    Expresses rsEGFP2 in bacterial cells

    Depositor

    Stefan Jakobs

    Article

    Grotjohann et al eLife. 2012 Dec 31:1:e00248. doi: 10.755

    Insert

    rsEGFP2
    

    Tags

    6xHisTag

    Expression

    Bacterial

    Available Since

    Jan. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. XRE-H2B-mScarlet-I

    Plasmid

    #182296

    Purpose

    Fluorescent reporter for AhR activity

    Depositor

    Severine Degrelle, Thierry Fournier

    Article

    Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

    Insert

    mScarlet-I
    

    Tags

    m-Scarlet-I

    Expression

    Mammalian

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCEP4-191084-gp160

    Plasmid

    #123234

    Purpose

    Mammalian expression plasmid for Env from the 191084 B7-19 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (191084 B7-19) Env
    

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-CrmA

    Plasmid

    #11832

    Depositor

    Guy Salvesen

    Article

    Muzio et al J Biol Chem. 1997 Jan 31. 272(5):2952-6.

    Insert

    CrmA (CrmA Cowpox virus)
    

    Expression

    Mammalian

    Available Since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. GG-EBNA-EEA-5guides-PGK-Puro

    Plasmid

    #102898

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    EEA-5guides-PGK-Puro
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. mTurquoise2-Giantin

    Plasmid

    #129630

    Purpose

    In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    mTurquoise2-Giantin
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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