We narrowed to 15,118 results for: pET

1. pET21-HIS-Tol2

   Plasmid

   #112486

   Purpose

   functional recombinant Tol2 protein from E.coli

   Depositor

   Stephen Ekker

   Article

   Ni et al Mob DNA. 2016 Mar 31;7:6. doi: 10.1186/s

   Insert

   Tol2 Transposase (tpase Synthetic)
   

   Tags

   HIS/6x

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-MBP-Geo_st

   Plasmid

   #87700

   Purpose

   Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP

   Depositor

   Jennifer Doudna

   Insert

   GeoCas9
   

   Tags

   10xHis-MBP-TEV

   Expression

   Bacterial

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-LaM-4

   Plasmid

   #172770

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-4, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-4
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-sfGFP 150TAG

   Plasmid

   #85493

   Purpose

   pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

   Depositor

   Ryan Mehl

   Article

   Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

   Insert

   superfolder GFP 150TAG
   

   Expression

   Bacterial

   Mutation

   150 TAG (amino acid 150 mutated to stop codon)

   Promoter

   T7

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETSXM2-pLacI-mtrCAB

   Plasmid

   #174618

   Purpose

   Bacterial expression of gene cluster mtrCAB from Shewanella oneidensis MR-1.

   Depositor

   I-Son Ng

   Article

   I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

   Insert

   mtrCAB
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   pLacI

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pet42c(+)-GST-USP5

   Plasmid

   #61750

   Purpose

   express N-terminally GST tagged human USP5

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP5 (USP5 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pET28-2H12.D11

   Plasmid

   #221145

   Purpose

   His6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)

   Depositor

   Urs Jenal

   Article

   Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

   Insert

   cdGreen2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-tdTomato-CNA35

   Plasmid

   #61606

   Purpose

   bacterial expression of N-terminal fusion protein of CNA35 with tdTomato

   Depositor

   Maarten Merkx

   Article

   Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

   Insert

   tdTomato and CNA35
   

   Tags

   His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-SisTnpB1–ωRNA

   Plasmid

   #228365

   Purpose

   Expresses SisTnpB1 and RNP complex in E. coli Rosetta (DE3) cells

   Depositor

   Tao Liu

   Article

   Xu et al Commun Biol. 2024 Nov 20;7(1):1541. doi:

   Insert

   SisTnpB1
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-MinE-His

    Plasmid

    #133623

    Purpose

    Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

    Insert

    MinE-His (minE E. coli)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-mCherry-CNA35

    Plasmid

    #61607

    Purpose

    bacterial expression of N-terminal fusion protein of CNA35 with mCherry

    Depositor

    Maarten Merkx

    Article

    Aper et al PLoS One. 2014 Dec 9;9(12):e114983. doi:

    Insert

    mCherry and CNA35
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-XPO5-His6

    Plasmid

    #171562

    Purpose

    Encodes human XPO5 protein

    Depositor

    Yan Zeng

    Insert

    XPO5 (XPO5 Human)
    

    Expression

    Bacterial

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-45b(+)-Tn5

    Plasmid

    #112112

    Purpose

    Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

    Depositor

    Frank Pugh

    Article

    Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

    Insert

    Tn5
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    E54K E110K P242A L372P

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-Sac-Abeta(M1-42)

    Plasmid

    #71875

    Purpose

    Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

    Depositor

    Dominic Walsh

    Article

    Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

    Insert

    Abeta(M1-42) (APP Human)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET23a-H6-TEV-cSrc

    Plasmid

    #214233

    Purpose

    Bacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

    Depositor

    Neel Shah

    Article

    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

    Insert

    cSrc Kinase domain (SRC Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28c-F30-Broccoli

    Plasmid

    #66788

    Purpose

    Expresses Broccoli in the F30 scaffold in bacterial cells

    Depositor

    Samie Jaffrey

    Article

    Filonov et al Chem Biol. 2015 May 21;22(5):649-60. doi

    Insert

    Broccoli in the F30 scaffold
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pet26-MBP-ELIC

    Plasmid

    #39239

    Depositor

    Raimund Dutzler

    Article

    Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

    Insert

    Eriwinia chrysanthemi - pentameric ligand gated ion channel
    

    Tags

    His-10 and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET3a-OSF-CypA

    Plasmid

    #79039

    Purpose

    Bacterial vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OneSTrEP-FLAG- Cyclophilin A (PPIA Human)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET23a-H6-TEV-cAbl

    Plasmid

    #214234

    Purpose

    Bacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screenings

    Depositor

    Neel Shah

    Article

    Shah et al Elife. 2018 Mar 16;7. pii: 35190. doi: 1

    Insert

    ABL1 Kinase domain (ABL1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21a(+)-Bst LF-6xHis

    Plasmid

    #159148

    Purpose

    expresses His-tagged large fragment of Bst polymerase

    Depositor

    Andrea Pauli

    Article

    Kellner et al Front Mol Biosci. 2022 Apr 1;9:801309. d

    Insert

    large fragment of Geobacillus stearothermophilus DNA polymerase I
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 1, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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21. pET51b(+)_SNAP-tag2

    Plasmid

    #226512

    Purpose

    Bacterial cell expression of SNAP-tag2

    Depositor

    Kai Johnsson

    Article

    Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

    Insert

    SNAP-tag2
    

    Tags

    His-tag (10x) and Strep-Tag II,

    Expression

    Bacterial

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pET263-pUC57 24xMS2V7

    Plasmid

    #140705

    Purpose

    MS2V7 cloning

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V7
    

    Use

    Cloning vector

    Mutation

    24 MS2 loops V7, high-affinity C-variant, inter-s…

    Promoter

    None

    Available Since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET14b – E. coli Exonuclease I

    Plasmid

    #104552

    Purpose

    bacterial expression of Exonuclease I

    Depositor

    Charles Bell

    Article

    Korada et al Nucleic Acids Res. 2013 Jun;41(11):5887-

    Insert

    Exonuclease I (sbcB E.coli)
    

    Tags

    6X-His tag followed by thrombin site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. pET-Cas9-6xHis

    Plasmid

    #62374

    Purpose

    Expression of Cas9-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9_6xHis
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. pET3-ARF6-His6(CO)

    Plasmid

    #201887

    Purpose

    Used for bacterial expression of human protein ARF6

    Depositor

    Richard Kahn

    Insert

    ARF6 (ARF6 Human)
    

    Tags

    6xHis (GSGSGSHHHHHH)

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Available Since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET28a hMDH1v3 (+TEV)

    Plasmid

    #204229

    Purpose

    Bacterial expression of human cytosolic malate dehydrogenase isoform 1 transcript variant 3 (hMDH1v3); Use in CURES (integration of research into undergraduate teaching labs).

    Depositor

    Malate dehydrogenase CUREs Community (MCC)

    Insert

    hMDH1v3 (MDH1 Human)
    

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    Inserted a G after ATG to maintain kozak site

    Promoter

    Lac

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pET21 MBPx-SpyCatcher

    Plasmid

    #72327

    Purpose

    Bacterial expression of tandem high affinity maltose binding protein domains linked to SpyCatcher, for solid-phase polyprotein synthesis.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET21 MBPx-SpyCatcher
    

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Mutation

    MBP contains A312V, I317V mutations and 172, 173,…

    Available Since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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28. pET_RTX(exo-)-6xHis

    Plasmid

    #145028

    Purpose

    pET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tag

    Depositor

    Andrew Ellington

    Article

    Bhadra et al BioProtocol 2021

    Insert

    RTX(exo-)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N210D

    Promoter

    T7

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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29. p3990 pET ubcH8

    Plasmid

    #8650

    Depositor

    Peter Howley

    Article

    Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

    Insert

    UbcH8 (UBE2L6 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    July 18, 2005

    Availability

    Academic Institutions and Nonprofits only

30. pET28-his-3C_Tau352

    Plasmid

    #100092

    Purpose

    Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

    Depositor

    Nikolai Sluchanko

    Article

    Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

    Insert

    Fetal microtubule-associated protein tau (MAPT Human)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    wild type sequence

    Promoter

    T7

    Available Since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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