We narrowed to 46,640 results for: PLE
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pET28a SUMO Nup53xl 1-320
Plasmid#85998Purposebacterial expression of his6 and SUMO tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
TagsSUMOExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7Available SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSET-pr-Kaede (Kaede-A69T)
Plasmid#99228PurposeBacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).DepositorInsertpr-Kaede
ExpressionBacterialMutationA69T for primed conversion applicationsPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC2-dual-dCas9VP48-sgExpression
Plasmid#48236PurposeDual expression construct expressing both dCas9VP48 and sgRNA from separate promotersDepositorInsertdCas9VP48
UseCRISPRTagsHA-tag and VP48ExpressionMammalianMutationD10A H840A (catalytically inactive)Available SinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX335-EN475
Plasmid#86231PurposeFor transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN477.DepositorInsertspCas9-nickase and sgRNA against mouse CTCF STOP Codon
UseMouse TargetingExpressionMammalianAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pX335-EN477
Plasmid#86232PurposeFor transient expression of spCas9-nickase and one sgRNA targeting the mouse CTCF locus. Use together with pX335-EN475.DepositorInsertspCas9-nickase and sgRNA against mouse CTCF STOP Codon
UseMouse TargetingExpressionMammalianAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eGFP-NMHC-IIAΔtailpiece
Plasmid#35689DepositorInsertMHC IIA (MYH9 Human)
TagsEGFPExpressionMammalianMutationAdded stop codon after codon 1928, eliminating th…PromoterCMVAvailable SinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMGS36 (GFP-ARF16-PB1)
Plasmid#126581PurposeConstruct to express GFP-ARF16-PB1 under the control of the CMV promoterDepositorInsertOsARF16-PB1 domain
TagsGFPExpressionMammalianAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
G-GECO1-Orai3
Plasmid#73563PurposeGreen fluorescent reporter of Orai3-associated calcium influxDepositorInsertG-GECO1-Orai3 (ORAI3 Synthetic, Human)
TagsG-GECO1ExpressionMammalianPromoterCMV Immdeiate EarlyAvailable SinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-3xFlag-ETO
Plasmid#12507DepositorAvailable SinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
ECFP-Bcr
Plasmid#36415DepositorAvailable SinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
Plxna4-Fc-His
Plasmid#72125PurposeExpresses the extracellular region of the PlexinA4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
STAgR_Neo
Plasmid#102992PurposeCan be used as backbone for a STAgR reactionDepositorTypeEmpty backboneUseCRISPR; Pcr template for stagr vectorsPromoterU6Available SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRK5 BARK1 minigene
Plasmid#14695DepositorInsertBARK1 minigene (GRK2 Bovine)
Tags3'UTR of human b-globinExpressionMammalianMutationBARK1 amino acids 495-689Available SinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV14-mULK1
Plasmid#24301DepositorInserta novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)
Tags3xFLAGExpressionMammalianAvailable SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RAB-EKARev(T/A)
Plasmid#118475PurposeNegative-control mutant for RAB-EKARev biosensor.DepositorInsertRAB-EKARev(T/A)
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-Turbo-NES-YFP-STOP-L2
Plasmid#127358Purposegateway entry vector for expressing cytosolic TurboID-YFP under a promoter of choiceDepositorInsertTurboID (BirA mutant)
UseGateway CloningTagsGS linker, NES, V5, and YFPMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMOD_B2203
Plasmid#91066PurposeModule B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE CDepositorInsertSapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
UseCRISPRPromoterCmYLCVAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
D. vulgaris Cascade/I-C (Cas5c-Cas8c-Cas7)/pHMGWA
Plasmid#81185PurposeExpresses Desulfovibrio vulgaris Cascade/I-C complex subunits in E. coli with TEV-cleavable N-terminal His-MBP tag on Cas5cDepositorInsertsCas5c
Cas8c
Cas7
UseCRISPRTags6xHis - MBP - TEV protease siteExpressionBacterialPromoterT7Available SinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only
