We narrowed to 59,253 results for: {{{s}}}

1. pEGFP-FCHO1

   Plasmid

   #196910

   Purpose

   visualization of clathrin-coaed vesicle formation in living mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Sakaushi et al Biosci Biotechnol Biochem. 2007 Jul;71(7

   Insert

   FCHO1 (FCHO1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-ACBEmax

   Plasmid

   #139106

   Purpose

   Mammalian ACBEmax expression vector (pSI-632)

   Depositor

   Nozomu Yachie

   Article

   Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available Since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET19-MTdTc302

   Plasmid

   #207458

   Purpose

   Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302

   Depositor

   Jay Keasling

   Article

   Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

   Insert

   MTdTc302 (Dntt Mouse)
   

   Use

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   Cys188Ala, Cys216Ser, Cys378Ala, and Cys438Ser

   Promoter

   Available Since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21b-rrt-His8

   Plasmid

   #194088

   Purpose

   Expresses Ec86 reverse transcriptase in Escherichia coli

   Depositor

   Tingting Zou

   Article

   Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

   Insert

   Ec86 rrt
   

   Use

   Tags

   His8

   Expression

   Mutation

   Promoter

   T7

   Available Since

   March 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19.TRIM28.FKBP.knock-in.mRuby2

   Plasmid

   #185008

   Purpose

   For knocking in FKBP F36V P2A mRuby2

   Depositor

   Denes Hnisz

   Article

   Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

   Insert

   FKBP F36V P2A mRuby2
   

   Use

   Cloning

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pN1-CMV-L-sDarken

   Plasmid

   #184801

   Purpose

   Low affinity version of the Serotonin Sensor sDarken under the control of a CMV Promoter.

   Depositor

   Olivia Masseck

   Article

   Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

   Insert

   L-sDarken
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28_6xHis-PARP1-C125G

   Plasmid

   #174792

   Purpose

   Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-C125G (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   C125G, V762A

   Promoter

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Arrb1-Q394L

   Plasmid

   #35402

   Depositor

   Robert Lefkowitz

   Article

   Hara et al Nature. 2011 Aug 21;477(7364):349-53. do

   Insert

   beta-arrestin-1 (Arrb1 Rat)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Q394L

   Promoter

   CMV

   Available Since

   March 2, 2012

   Availability

   Academic Institutions and Nonprofits only

9. Dendra2-Vinculin-N-21

   Plasmid

   #57749

   Purpose

   Localization: Focal Adhesions, Excitation: 490 / 553, Emission: 507 / 573

   Depositor

   Michael Davidson

   Article

   Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   NM_003373.3 (VCL Human)
   

   Use

   Tags

   Dendra2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 6, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

10. pCMV/myc/ER-αT2ib

    Plasmid

    #110769

    Purpose

    Expresses ER intrabody against mouse and human TLR2

    Depositor

    Thomas Böldicke

    Article

    Kirschning et al BMC Biotechnol. 2010 Apr 13;10:31. doi:

    Insert

    intrabody against mouse and human TLR2
    

    Use

    Tags

    ER retention signal (SEKDEL), ER signal peptide, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1P POLE1_1

    Plasmid

    #160762

    Purpose

    Suppress POLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV Gpat4-V5

    Plasmid

    #175145

    Purpose

    Lentiviral expression of mouse Gpat4-V5

    Depositor

    Larry Gerace

    Article

    Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

    Insert

    Gpat4 (Gpat4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. MIGR1-CD5

    Plasmid

    #159329

    Purpose

    Over-expression of mouse CD5

    Depositor

    Nevil Singh

    Article

    Matson et al Proc Natl Acad Sci U S A. 2020 Jun 23;11

    Insert

    CD5 (Cd5 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    LTR

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. LentiV_Neo_Sik3_T142Q

    Plasmid

    #119973

    Purpose

    Lentiviral expression plasmid of mouse Sik3 cDNA (gatekeeper mutation) with neomycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Blood. 2019 Nov 1. pii: 422697. doi: 10.

    Insert

    Sik3 (Sik3 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    change Threonine 142 to Glutamine

    Promoter

    EFS promoter

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-EF1a-V5-Lem2

    Plasmid

    #120245

    Purpose

    Expresses V5-tagged Lem2 in lentiviral vector

    Depositor

    Larry Gerace

    Article

    Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

    Insert

    Lem2 (Lemd2 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGB3_alpha2: Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos-Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1679)

    Plasmid

    #160619

    Purpose

    Module for estradiol-inducible expression of the PhiC31 integrase gene and dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    ERLexABDGal4AD / PhiC31 / GRLacIBDGal4AD / RDF
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available Since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII

    Plasmid

    #217896

    Purpose

    Insect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tag

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    PSMB5 (PSMB5 Human)
    PSMB6 (PSMB6 Human)
    PSMB7 (PSMB7 Human)
    

    Use

    Tags

    2xSTII

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-HA-mBec1

    Plasmid

    #99505

    Purpose

    3rd generation lentiviral expression of wild-type Becn1

    Depositor

    Congcong He

    Article

    Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

    Insert

    Beclin 1 (Becn1 Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. SspB-iRFP-DHPH-ITSN1

    Plasmid

    #176117

    Purpose

    Heterodimerization with iLID, Cdc42 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    ITSN1 (ITSN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1

    Plasmid

    #123194

    Purpose

    binary plant vector for transient dual luciferase (rLUC & fLUC with introns) expression

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    pNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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