We narrowed to 7,871 results for: 199

1. pCMV-myc-ERK2-MEK1_fusion

   Plasmid

   #39194

   Depositor

   Melanie Cobb

   Article

   Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

   Insert

   ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pET-His6-ERK2-MEK1_R4F_coexpression

   Plasmid

   #39212

   Depositor

   Melanie Cobb

   Article

   Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

   Inserts

   Mek1 R4F (MAP2K1 Human)
   Erk2 (Mapk1 Rat)
   

   Use

   Tags

   His6 and RBS

   Expression

   Bacterial

   Mutation

   MEK1(R4F) = S218E, S222D and deletion of AA32-51

   Promoter

   T7 and T7 via RBS

   Available since

   July 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-myc-ERK2-L4A-MEK1_fusion

   Plasmid

   #39197

   Depositor

   Melanie Cobb

   Article

   Robinson et al Curr Biol. 1998 Oct 22;8(21):1141-50.

   Insert

   ERK2-MEK1 Fusion (MAP2K1, Mapk1 Human, Rat)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   L4A = L33A, L37A, L40A, L42A

   Promoter

   CMV

   Available since

   July 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-MKK1

   Plasmid

   #107973

   Purpose

   expression of MAP Kinase Kinase

   Depositor

   Edwin Krebs

   Article

   Seger et al J Biol Chem. 1994 Oct 14;269(41):25699-7

   Insert

   MKK1 (MAP2K1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   See depositor comments below.

   Promoter

   CMV

   Available since

   Feb. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-MKK1K97A

   Plasmid

   #107974

   Purpose

   expression of a dominant negative MAP Kinase Kinase

   Depositor

   Edwin Krebs

   Article

   Seger et al J Biol Chem. 1994 Oct 14;269(41):25699-7

   Insert

   MKK1 K97A (MAP2K1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   dominant negative; see Depositor Comments below

   Promoter

   CMV

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Rb379-928/C706F-pGex2TK

   Plasmid

   #21750

   Depositor

   William Kaelin

   Article

   Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deletion mutant ORF contains aa 379-928 of wt Rb …

   Promoter

   Available since

   Sept. 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-U6sgp53-mTSG-GFAPCre

   Plasmid

   #100276

   Purpose

   AAV-CRISPR library for pool mutagenesis of top tumor suppressor genes

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

   Insert

   GFAP promoter (Gfap, GFAP Human, Mouse)
   

   Use

   AAV, CRISPR, Mouse Targeting, and Synthetic Biolo…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSYC-201

   Plasmid

   #178963

   Purpose

   Human GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombination

   Depositor

   Seok-Yong Choi

   Article

   Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1

   Inserts

   Glial fibrillary acidic protein promoter (gfap Zebrafish)
   Glial fibrillary acidic protein (GFAP Human)
   EGFP-pA
   

   Use

   Tol2 mediated recombination

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   zebrafish gfap promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSYC-204

   Plasmid

   #178964

   Purpose

   Human GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombination

   Depositor

   Seok-Yong Choi

   Article

   Lee et al BMC Neurol. 2017 Sep 7;17(1):175. doi: 1

   Inserts

   Glial fibrillary acidic protein promoter (gfap Zebrafish)
   Glial fibrillary acidic protein (GFAP Human)
   EGFP-pA
   

   Use

   Tol2 mediated recombination

   Tags

   3xFLAG

   Expression

   Mutation

   R79C

   Promoter

   Zebrafish gfap promoter

   Available since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1 His-V5 Myc dMBIIa

    Plasmid

    #176053

    Purpose

    Expresses Myc Mutant (delta Myc BoxIIa) tagged with His and V5 in Mammalian cells

    Depositor

    Linda Penn

    Article

    Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

    Insert

    Myc
    

    Use

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    deleted amino acids 188-199

    Promoter

    CMV

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX2T-TEV CHMP1B 4-178

    Plasmid

    #108284

    Purpose

    Expresses residues 4-178 of CHMP1B with an N-terminal GST tag and a TEV cleavage site; Internal ID: WISP 09-279

    Depositor

    Wesley Sundquist

    Article

    Nguyen et al Nat Struct Mol Biol. 2020 Apr;27(4):392-

    Insert

    CHMP1B (CHMP1B Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-3 and 179-199

    Promoter

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pOCC102-MBP-eIF4G-Sk

    Plasmid

    #219976

    Purpose

    eIF4G-Sk protein purification

    Depositor

    Simon Alberti

    Insert

    eIF4G-Sk
    

    Use

    Tags

    6xHis-MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAIP4_3HNX

    Plasmid

    #234199

    Purpose

    Heterologous protein expression of cyanovirin-N homolog (CV-N homolog) in Escherichia coli

    Depositor

    Christopher Bahl

    Insert

    3HNX
    

    Use

    Tags

    10x HisTag-Smt3

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 6, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. mMaroon-CytERM-N-17

    Plasmid

    #56199

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 610, Emission: 652

    Depositor

    Michael Davidson

    Insert

    CytERM
    

    Use

    Tags

    mMaroon

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pRDAX-VALENTIA-018 NLSNoLS-Eos//BsaI

    Plasmid

    #130199

    Purpose

    EMMA Kit # 1000000119 extension

    Depositor

    Gary Lewin

    Insert

    NLSNoLS-Eos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-TRAF1

    Plasmid

    #111199

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Insert

    TRAF1 (TRAF1 Human)
    

    Use

    Tags

    EGFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mEmerald-N-Wasp-C-18

    Plasmid

    #54199

    Purpose

    Localization: Cytoskeleton-Signalling , Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    N-Wasp
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pOCC102-MBP-eIF4G

    Plasmid

    #219971

    Purpose

    eIF4G protein purification

    Depositor

    Simon Alberti

    Insert

    eIF4G (TIF4631 Budding Yeast)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCfb12567

    Plasmid

    #219927

    Purpose

    PrCYC with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

    Depositor

    Irina Borodina

    Insert

    Promoter CYC
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfb12566

    Plasmid

    #219926

    Purpose

    PrDGA with the overhangs for SapI restriction site can be insert into the base plasmid of TUNEYALI

    Depositor

    Irina Borodina

    Insert

    Promoter DGA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge