We narrowed to 5,041 results for: U6...

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pPN167

Plasmid

#91586

Purpose

Express sgRNA targeting human CNTN4

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

CNTN4 (CNTN4 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

Plasmid

#68356

Purpose

-The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter

Depositor

Jannik Elverløv-Jakobsen

Inserts

4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
FlpO recombinase

Use

CRISPR

Tags

mTurquoise2 (BFP)

Expression

Mammalian

Promoter

Synthetic Probasin ARRx2 and U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (5'GAC)

Plasmid

#170127

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(5'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (3'GAC)

Plasmid

#170129

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a GAC at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'GAC)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Availability

Academic Institutions and Nonprofits only

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p-AAV-sh[control]

Plasmid

#75438

Purpose

control shRNA vector

Depositor

Edward Burton

Article

Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

Insert

GFP

Use

AAV and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry GFP

Plasmid

#78535

Purpose

Control plasmid (paired gRNAs against GFP)

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA) and U6 promoter

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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BPK1520

Plasmid

#65777

Purpose

Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SpCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCV-U6sgRNA(BbsI)-PGKpuro2ABFP

Plasmid

#102796

Purpose

Retrovirus for delivery of one sgRNA (empty, bbsI)

Depositor

Sarah Teichmann

Article

Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

Insert

U6 sgRNA

Use

CRISPR and Retroviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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Pepper-fused sgRNA

Plasmid

#217520

Purpose

Expresses Pepper-fused sgRNA in mammalian cells with U6 promoter

Depositor

Xing Li

Article

Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

Insert

Pepper-fused sgRNA for spCas9

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

Plasmid

#108871

Purpose

Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

Depositor

Alex Schier

Article

Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

Inserts

5xU6:sgRNA
Cas9-t2A-GFP

Use

CRISPR

Promoter

U6 and hsp70

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pX330-gRNA

Plasmid

#158973

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

Depositor

Charles P. Lai

Article

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Insert

gRNA3

Expression

Mammalian

Promoter

U6

Available Since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pEJS613_pTetR-P2A-BFPnls/sgTelo

Plasmid

#108649

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Telomere-targeting sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgSTAT3-1

Plasmid

#121425

Purpose

sgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Ovijit Chaudhuri, Stanley Qi

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

sgSTAT3-1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

mouse U6

Available Since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-EEA-5guides-PGK-Puro

Plasmid

#102898

Purpose

EBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

EEA-5guides-PGK-Puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-OSK2M2L1-PP

Plasmid

#102902

Purpose

EBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

OSK2M2L1-gRNAs-PGK-puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G8_Dual_sgRNA

Plasmid

#178104

Purpose

Coselection for PE3 in human cells. Vector for tandem expression of ATP1A1 G8 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G8 sgRNA + user-specified sgRNA

Use

CRISPR; Prime editing

Expression

Mammalian

Promoter

Tandem U6 promoters

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pEJS614_pTetR-P2A-BFPnls/sgNS

Plasmid

#108650

Purpose

Non-specific Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Non-specific sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pJRH051

Plasmid

#171625

Purpose

2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA (no spacer, BsmBI cutsites) and an EF1-a promoter expressing mNeonGreen

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Inserts

spyCas9 sgRNA-BsmBI-Destination
mNeon

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1-a and U6

Available Since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

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pX330_DRS-1 sgRNA / hSpCas9

Plasmid

#172841

Purpose

Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pKRG3-mU6-PUb-hSpCas9

Plasmid

#162162

Purpose

Expresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)

Depositor

Charles Rice

Article

Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

Inserts

hSpCas9
sgRNA

Use

CRISPR

Tags

N/A

Expression

Insect

Promoter

Ae. aegypti PUb and Ae. aegypti U6

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

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